Chemometric-enhanced metabolic profiling of five Pinus species using HPLC-MS/MS spectrometry: Correlation to in vitro anti-aging, anti-Alzheimer and antidiabetic activities

Saber, Fatema R.; Mohsen, Engy; El‐Hawary, Seham S.; Eltanany, Basma M.; Elimam, Hanan; Sobeh, Mansour; Elmotayam, Amira K.

doi:10.1016/j.jchromb.2021.122759

Cited by 17 publications

(7 citation statements)

References 54 publications

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“…In our study, AgNPs were prepared using A. alba and P. sylvestris bark aqueous extracts. Both extracts were initially subjected to LC-HRMS/MS resulting in the identification/tentative identification of 22 compounds belonging to various phytochemical classes [ 25 , 30 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 ]. Of these compounds, several phenolics have already been reported in both conifer barks: catechin, gallocatechin, epicatechin, and lariciresinol in A. alba bark [ 23 , 76 ] and O -hexosides ( O -glucosides) of hydroxybenzoic acid, vanillic acid, and taxifolin, procyanidin dimer, catechin, and taxifolin in P. sylvestris bark [ 21 , 25 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Silver Nanoparticles Synthesized from Abies alba and Pinus sylvestris Bark Extracts: Characterization, Antioxidant, Cytotoxic, and Antibacterial Effects

et al. 2023

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In recent years, phytofunctionalized AgNPs have attracted great interest due to their remarkable biological activities. In the present study, AgNPs were synthesized using Abies alba and Pinus sylvestris bark extracts. The chemical profile of these bark extracts was analyzed by LC-HRMS/MS. As a first step, the synthesis parameters (pH, AgNO3 concentration, ratio of bark extract and AgNO3, temperature, and reaction time) were optimized. The synthesized AgNPs were characterized by ATR-FTIR spectroscopy, DLS, SEM, EDX, and TEM. Their antioxidant, cytotoxic, and antibacterial properties were evaluated by the DPPH, ABTS, MTT, and broth microdilution assays, respectively. Abies alba and Pinus sylvestris bark extract-derived AgNPs were well-dispersed, spherical, small (average particle size of 9.92 and 24.49 nm, respectively), stable (zeta potential values of −10.9 and −10.8 mV, respectively), and cytotoxic to A-375 human malignant melanoma cells (IC50 = 2.40 ± 0.21 and 6.02 ± 0.61 μg/mL, respectively). The phytosynthesized AgNPs also showed antioxidant and antibacterial effects.

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Section: Discussionmentioning

confidence: 99%

Silver Nanoparticles Synthesized from Abies alba and Pinus sylvestris Bark Extracts: Characterization, Antioxidant, Cytotoxic, and Antibacterial Effects

et al. 2023

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“…However, with the encapsulation of PNE in coating the α-amylase inhibition activity increased significantly from 10.23 ± 0.63 % to 16.80 ± 0.01 %. The antidiabetic potential of pine needles could be attributed to the presence of phenolics and diterpenoids [31] . Singh, Kumar, & Dash [32] reported considerable fall in fasting glucose levels in diabetic rats upon incorporation of pine enriched oral treatment.…”

Section: Resultsmentioning

confidence: 99%

Ultrasonics as a tool for development of pine-needle extract loaded bee wax edible packaging for value addition of Himalayan cheese

Jenno

Noor

Mushtaq

et al. 2022

Ultrasonics Sonochemistry

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“…Eleven flavonoid aglycones were detected in peaks 41, 46, 66, 69, 74, 75, 76, 79, 80, 83, and 84 with molecular ion peaks at m / z 305.0639, 319.1014, 287.0536, 285.1326, 269.1132, 303.0495, 315.0496, 269.1017, 289.1795, 317.1166, 301.1427, corresponding to taxifolin, myricetin, luteolin, acacetin, formononetin, quercetin, isorhamnetin, apigenin, eriodictyol, rhamnetin, and kaempferide, respectively. Taxifolin (peak 41) was characterized by a molecular ion peak at m / z 305.0639 with distinctive fragment ions at m / z 287.0832 [(M + H)-H2O] [ 53 ]. Isorhamnetin (peak 76) displayed a pseudo molecular ion [M − H] − at m / z 315.0496 with main fragment ions at m / z 300.0077 and m / z 301.1315 corresponding to loss of CH 3 and CH 2 , respectively [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Novel Neuroprotective Potential of Bunchosia armeniaca (Cav.) DC against Lipopolysaccharide Induced Alzheimer’s Disease in Mice

Abbas

Salama

El‐Toumy

et al. 2022

Plants

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Bunchosia armeniaca (Cav.) DC (Malpighiaceae) is one of the well-known traditionally used remedies worldwide. This study aims to explore the leaves’ metabolome via Quadrupole-Time-of-Flight-Liquid-Chromatography-Mass Spectrometry and to investigate the neuroprotective effect of leaves using lipopolysaccharide (LPS) induced Alzheimer’s disease model. Mice were administered LPS (0.25 mg/kg/day; intraperitoneal) as well as methanolic extract (BME), dichloromethane (BDMF), and butanol (BBF) fractions (each 200 mg/kg/day; oral) for one week. BME and BBF improved behavioral activity on the Y maze test, decreased brain content of inflammatory markers such as nuclear factor kappa B and interleukin 1 beta, and prevented the elevation of cytochrome P450 2E1, and glial fibrillary acidic protein compared to the LPS-administered group. Histopathological examination of several brain parts confirmed the neuroprotective effect of the tested extracts. In addition, BBF exhibited higher activity in all tested in vitro antioxidant and acetylcholinesterase inhibition assays. Metabolic profiling offered tentative identification of 88 metabolites, including mainly flavonoids, phenolic acids, and coumarins. Several detected metabolites, such as quercetin, apigenin, baicalin, vitexin, and resveratrol, had previously known neuroprotective effects. The current study highlighted the possible novel potential of B. armeniaca in preventing memory impairment, possibly through its antioxidant effect and inhibition of acetylcholinesterase, inflammatory and oxidative stress mediators.

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Chemometric-enhanced metabolic profiling of five Pinus species using HPLC-MS/MS spectrometry: Correlation to in vitro anti-aging, anti-Alzheimer and antidiabetic activities

Cited by 17 publications

References 54 publications

Silver Nanoparticles Synthesized from Abies alba and Pinus sylvestris Bark Extracts: Characterization, Antioxidant, Cytotoxic, and Antibacterial Effects

Silver Nanoparticles Synthesized from Abies alba and Pinus sylvestris Bark Extracts: Characterization, Antioxidant, Cytotoxic, and Antibacterial Effects

Ultrasonics as a tool for development of pine-needle extract loaded bee wax edible packaging for value addition of Himalayan cheese

Novel Neuroprotective Potential of Bunchosia armeniaca (Cav.) DC against Lipopolysaccharide Induced Alzheimer’s Disease in Mice

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