Chemomics‐Integrated Proteomics Analysis of Jie‐Geng‐Tang to Ameliorate Lipopolysaccharide‐Induced Acute Lung Injury in Mice

Abstract

Jie-Geng-Tang (JGT), a classic and famous traditional Chinese medicine (TCM) prescription composed of Platycodon grandiflorum (Jacq.) A. DC. (PG) and Glycyrrhiza uralensis Fisch. (GU), is well known for “clearing heat and relieving toxicity” and its ability to “diffuse the lung and relieve sore throat.” However, the mechanism underlying its action remains unclear. In this study, potential anti-inflammatory ingredients were screened and submitted to PharmMapper and the KEGG bioinformatics website to predict the target proteins and related pathways, respectively. Differentially expressed candidate proteins from acute lung injury (ALI) mice treated with JGT were identified by isobaric tags for relative and absolute quantitation (iTRAQ) and LC Triple-TOF. Eleven potential anti-inflammatory ingredients were found, including the derivatives of glycyrrhizic acid, licorice-saponin, liquiritin, and platycodigenin. A total of sixty-seven differentially expressed proteins were confirmed after JGT treatment with four therapeutic functions, including immunoregulation, anti-inflammation, ribosome, and muscle contraction. PG and GU comediate PI3K/Akt signal pathway inhibition of NF-κB, VCAM1, and ICAM1 release which primarily act on PI3K, PDK1, AKT, and GSK3β. GU markedly inhibits the ERK/MAPK signaling pathways and primarily acts on LCK, RAS, and MEK. A network was constructed using bioactive ingredients, targets, and pathways to determine the mechanism underlying JGT treatment of ALI.

Platycodon grandiflorum Saponins Ameliorate Cisplatin-Induced Acute Nephrotoxicity through the NF-κB-Mediated Inflammation and PI3K/Akt/Apoptosis Signaling Pathways

“…PGS and triterpenoid saponins isolated from the roots of P. grandiflorum have the advantage of a simple extraction method with low costs. Studies have illustrated the anti-inflammatory and anti-apoptotic activities of PGS in multiple organs [ 33 , 34 ]. Although several studies have clearly clarified that Platycodin D (PD) as a maker of saponin was used to protect against cisplatin-induced nephrotoxicity in mice, there is currently no simple and rapid method to isolate PD.…”

Novel insight into the genetic basis of high-altitude pulmonary hypertension in Kyrgyz highlanders

“…Furthermore, its role in allergic lung diseases [45] or in systemic sclerosis [46], where PAH is the leading cause of death [47], clearly indicates evidence of a positive selection sweep on genetic variants that would protect the No-HAPH subjects from developing PH. Furthermore, studies in mice with Lipopolysaccharide-induced acute lung injury have clearly shown an upregulation of VCAM1 [48]. Similarly, transcriptome analysis of patients diagnosed with HA pulmonary edema (HAPE), also an acute lung injury, depicts maximum fold change for VCAM1 along with MAPK10 [49].…”

Non-targeted proteomics of acute respiratory distress syndrome: clinical and research applications

“…Therefore, the authors suggested that doxycycline may prevent or treat high tidal volume ventilation-induced ALI by inhibiting the activity of matrix metalloproteinase-9. Certain Chinese medicine preparations such as ShuFengJieDu Capsule [ 34 ] and Jie-Geng-Tang [ 32 ] have certain therapeutic effects on ARDS, but the mechanism is unknown. Proteomic research can explain the possible potential effects of drugs.…”