Chemoprevention by curcumin during the promotion stage of tumorigenesis of mammary gland in rats irradiated with gamma-rays

Itoh, Hiroshi; Onoda, Makoto; Inafuku, Naoshi; Kubota, Megumi; Kamada, Yasuhiro; Osawa, Toshihiko; Kobayashi, Hill Hiroki; Wakabayashi, Keiji

doi:10.1093/carcin/20.6.1011

Cited by 101 publications

(41 citation statements)

References 60 publications

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“…DISCUSSION Curcumin has been shown to have chemopreventive effects on breast cancer. 20,21 Recently, Inano et al have reported that curcumin has a potent preventive activity during the DES-dependent promotion stage of radiation-induced mammary tumorigenesis. 20 In our study, we provide evidence that curcumin exerts multiple different suppressive effects on human breast carcinoma cells in vitro.…”

Section: Curcumin's Modulation Of Angiogenesis Factor Expressionmentioning

confidence: 99%

Curcumin exerts multiple suppressive effects on human breast carcinoma cells

Shao

Shen

Liu

et al. 2001

Intl Journal of Cancer

362

261

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In our study, we present experimental evidence suggesting that curcumin exerts multiple different suppressive effects on human breast carcinoma cells in vitro. Our experiments demonstrate that curcumin's antiproliferative effects are estrogen dependent in ER (estrogen receptor)-positive MCF-7 cells, being more pronounced in estrogen-containing media and in the presence of exogenous 17-␤ estradiol. Curcumin inhibits the expression of ER downstream genes including pS2 and TGF-␤ (transforming growth factor) in ER-positive MCF-7 cells, and this inhibition is also dependent on the presence of estrogen. Curcumin also decreases ERE (estrogen responsive element)-CAT activities induced by 17-␤ estradiol. In addition, we demonstrate that curcumin exerts strong antiinvasive effects in vitro that are not estrogen dependent in the ER-negative MDA-MB-231 breast cancer cells. These antiinvasive effects appear to be mediated through the downregulation of MMP-2 (matrix metalloproteinase) and the upregulation of TIMP-1 (tissue inhibitor of metalloproteinase), 2 common effector molecules that have been implicated in regulating tumor cell invasion. Our study also demonstrates that curcumin inhibits the transcript levels of 2 major angiogenesis factors VEGF (vascular endothelial growth factor) and b-FGF (basic fibroblast growth factor) mainly in ER-negative MDA-MB-231 cells. © 2002 Wiley-Liss, Inc. Key words: curcumin; chemoprevention; breast cancer; angiogenesis; estrogenBreast cancer chemoprevention is the subject of substantial research efforts to improve the health of women in the United States. Epidemiologic surveys suggest that diet has an impact on cancer incidence. Frequent consumption of vegetables and fruits decreases the risk for human cancer. 1,2 Although risk reduction by nutritional intervention alone may not be sufficient to protect high-risk individuals against cancer development, it would be very useful to identify agents with chemopreventive potency and to evaluate them in combination with nutritional intervention. 3,4 Recently, attention has been focused on identifying dietary phytochemicals that have the ability to inhibit the processes of carcinogenesis. Extracts of plants or their fractionated ingredients are found to possess inhibitory effects against chemically induced carcinogenesis. 5 Curcumin is a major component of turmeric, the dried rhizome of Curcuma longa L., which is commonly used as a yellow coloring and flavoring agent in food items in Asian countries. Commercial-grade curcumin has shown anticarcinogenic activity in animals as indicated by the ability to block colon tumor initiation induced by azoxymethans 6 and skin tumor promotion induced by phorbol ester. 7 Curcumin also has been reported to possess antiinflammatory activity and is a potent inhibitor of reactive oxygen-generating enzymes such as lipooxygenase/cyclooxygenase, 8,9 xanthine dehydroxygenase/oxidase 10 and nitric oxide synthase (NOS). 11,12 Recently, it has been shown that the administration of synthetic curcumin in the diet during t...

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Section: Curcumin's Modulation Of Angiogenesis Factor Expressionmentioning

confidence: 99%

Curcumin exerts multiple suppressive effects on human breast carcinoma cells

Shao

Shen

Liu

et al. 2001

Intl Journal of Cancer

362

261

View full text Add to dashboard Cite

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“…In each experiment, 100 µL aliquots of growth medium, containing exponentially growing cells, were placed into 96-well plates, at 1.0 × 10 5 cells per well, then incubated for 24 hours. Subsequently, HepG2 cells were incubated with various formulations of curcumin (at concentrations of 0, 4,6,8,10,12,16, and 20 µΜ, with eight wells per concentration). After 48 hours of incubation, MTT solution (5 mg/mL) was added to each well.…”

Section: Mtt Assaymentioning

confidence: 99%

Chemotherapeutic potential of curcumin-bearing microcells against hepatocellular carcinoma in model animals

Farazuddin¹,

Dua²,

Zia³

et al. 2014

IJN

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Curcumin (diferuloylmethane) is found in large quantities in the roots of Curcuma longa. It possesses strong antioxidant and anti-inflammatory properties, and inhibits chemically-induced carcinogenesis in the skin, forestomach, colon, and liver. Unfortunately, the poor bioavailability and hydrophobicity of curcumin pose a major hurdle to its use as a potent anticancer agent. To circumvent some of these problems, we developed a novel, dual-core microcell formulation of curcumin. The encapsulation of curcumin in microcells increases its solubility and bioavailability, and facilitates slow release kinetics over extended periods. Besides being safe, these formulations do not bear any toxicity constraints, as revealed by in vitro and in vivo studies. Histopathological analysis revealed that curcumin-bearing microcells helped in regression of hepatocellular carcinoma and the maintenance of cellular architecture in liver tissue. Free curcumin had a very mild effect on cancer suppression. Empty (sham) microcells and microparticles failed to inhibit cancer cells. The novel curcumin formulation was found to suppress hepatocellular carcinoma efficiently in Swiss albino mice.

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“…It is of particular interest to note that Curcuma longa L is a fungus and fungi are well known for their high copper content. Since 13-diketones are well known chelating agents [123,124] and this fully conjugated [5- [126] reported that feeding Curcumin decreased the incidence of marnmm3' tumor m rats. A 1% Curcumin diet fed to 2.6 Gy whole-body irradiated pregnant Wistar-MS rats just prior to pup birth (a mammary tumor imtiating procedure) and then implanted with diethylstilbestrol sustained release pellets for 12 mo following weaning (a mammary tumor promoter regimen) caused a dramatically decreased incidence of mammary tumor.…”

Section: Chemical Consequences Of Ionizing Radiationmentioning

confidence: 99%

Essential Metalloelement Chelates Facilitate Repair of RadiationInjury

et al. 2001

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Treatment with essential metalloelement (Cu, Fe, Mn, and Zn) chelates or combinations of them before and/or after radiation injury is a useful approach to overcoming radiation injury. No other agents are known to increase survival when they are used to treat after irradiation, in a radiorecovery treatment paradigm. These chelates may be useful in facilitating de novo syntheses of essential metalloelement-dependent enzymes required to repair radiation injury. Reports of radioprotection, which involves treatment before irradiation, with calcium-.channel blockers, acyl Melatonin homologs, and substituted anilines, which may serve as chelating agents after biochemical modification in vivo, as well as Curcumin, which is a chelating agent, have been included in this review. These inclusions are intended to suggest additional approaches to combination treatments that may be useful in facilitating radiation recovery. These approaches to radioprotection and radiorecovery offer promise in facilitating recovery from radiation-induced injury experienced by patients undergoing radiotherapy for neoplastic disease and by individuals who experience environmental, occupational, or accidental exposure to ultraviolet, x-ray, or ,-ray radiation. Since there are no existing treatments of radiation-injury intended to facilitate tissue repair, studies of essential metalloelement chelates and combinations of them, as well as combinations of them with existing organic radioprotectants, seem worthwhile.

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Chemoprevention by curcumin during the promotion stage of tumorigenesis of mammary gland in rats irradiated with gamma-rays

Cited by 101 publications

References 60 publications

Curcumin exerts multiple suppressive effects on human breast carcinoma cells

Curcumin exerts multiple suppressive effects on human breast carcinoma cells

Chemotherapeutic potential of curcumin-bearing microcells against hepatocellular carcinoma in model animals

Essential Metalloelement Chelates Facilitate Repair of RadiationInjury

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