Chemopreventive activity of <i>Azadirachta indica</i> on two‐stage skin carcinogenesis in murine model

Arora, Neha; Koul, Ashwani; Bansal, M. P.

doi:10.1002/ptr.3280

Cited by 36 publications

(23 citation statements)

References 42 publications

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“…Alanine and aspartate transaminases (ALT; AST), creatinine, albumin and globulin levels were not affected, but urea levels increased. The number of circulating lymphocytes were also raised (Table 2), which could be accredited in part to the immunomodulatory action of plant polysaccharides (Arora et al, 2011). In conclusion, TPL and FI fractioned from A. indica seed tegument exhibited potent anti-inflammatory activity, interfering not only in vascular, but especially in cellular inflammatory events, involving serotonin, PGE 2 and NO.…”

Section: Resultsmentioning

confidence: 88%

“…In folk medicine, oil seeds, bark and leaf extracts have been used to treat gastrointestinal, analgesic and inflammatory disorders. Experimental data demonstrated immunomodulator and anti-infl ammatory activities of bark and leave extracts and also antipyretic and anti-infl ammatory effects of oil seeds (Arora et al, 2011;Biswas et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Anti-inflammatory polysaccharides of Azadirachta indica seed tegument

Pereira¹,

Silva²,

Silva³

et al. 2012

Rev. bras. farmacogn.

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Azadirachta indica A. Juss., Meliaceae, or Indian neem is a plant used to treat inflammatory disorders. Total polysaccharide (TPL) and FI (fractioned by ion exchange chromatography) from the seed tegument of A. indica were evaluated in models of acute inflammation (paw edema/peritonitis) using Wistar rats. Paw edema (measured by hydroplethysmometry) was induced s.c. by λ-carrageenan (300 μg), histamine (100 μg), serotonin (20 μg), compound 48/80 (10 μg), prostaglandin (PGE 2 30 μg) or L-arginine (15 μg). Peritonitis (analyzed for leukocyte counts/protein dosage) was induced i.p. by carrageenan (500 mg) or N-formyl-methionyl-leucyl-phenylalanine (fMLP 50 ng). Animals were treated i.v. with TPL (1 mg/kg) or FI (0.01, 0.1, 1 mg/kg) 30 min before stimuli. FI toxicity (at 0.1 mg/kg, i.v. for seven days) was analyzed by the variation of body/organ mass and hematological/biochemical parameters. TPL extraction yielded 1.3%; FI, presenting high carbohydrate and low protein content, at 0.1 mg/kg inhibited paw edema induced by carrageenan (77%), serotonin (54%), PGE 2 (69%) and nitric oxide (73%), and the peritonitis elicited by carrageenan (48%) or fMLP (67%), being well tolerated by animals. FI exhibited potent anti-inflammatory activity, revealing to be important active component in traditionally prepared remedies to treat inflammatory states.

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Section: Resultsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory polysaccharides of Azadirachta indica seed tegument

Pereira¹,

Silva²,

Silva³

et al. 2012

Rev. bras. farmacogn.

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“…Aloe vera administration may be responsible for suppression of oxidative stress induced by X‐ray irradiation (Dadupandhi, ). It has been documented that ionising radiation induces metabolic oxidative stress and apoptosis in the dividing cells (Agrawal & Kale, ; Arora et al, ). Previous studies showed TUNEL‐positive cells or bodies in irradiated animals (Jiang et al., ; Kim et al., ).…”

Section: Discussionmentioning

confidence: 99%

Protective role ofAloe veraagainst X-ray induced testicular dysfunction

et al. 2016

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The present investigation was carried out to evaluate the possible radioprotective potential of an Aloe vera extract against whole-body X-ray irradiation-induced testicular alterations in mice. Male balb/c mice were divided into four groups: control, A. vera, X-ray and A. vera pre-treated + X-ray irradiated. Histopathological examination revealed significant structural alterations in testes after X-ray exposure, which was also associated with the presence of apoptotic cells as assessed by TUNEL assay. X-ray irradiation resulted in elevation in the levels of reactive oxygen species, lipid peroxidation, a reduction in glutathione concentration and enhanced activities of antioxidant enzymes such as glutathione reductase, glutathione peroxidase, catalase, superoxide dismutase and glutathione-S-transferase. Sperm count/motility and testosterone levels were significantly decreased in the irradiated group. Irradiated animals pre-treated with A. vera extract revealed an improvement in antioxidant status, inhibition of lipid peroxides, apoptotic cell formation and enhanced testicular parameters when compared to the X-ray-exposed group. These findings suggest that A. vera extract could ameliorate X-ray-induced damage due to its free radical scavenging properties and its potential to boost cellular antioxidant defence machinery.

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“…Also, at the time point of 10 weeks (i.e. after 8 weeks of DMBA/TPA treatment when the papillomas begin to appear), it was observed that the skin of AAILE + DMBA/TPA‐treated animals exhibited an increase in lipid peroxidation levels when compared to the DMBA/TPA‐treated skin. Although this increase was statistically non‐significant, it may be emphasized that A. indica persistently keeps the lipid peroxidation levels high, in an attempt to prevent the damaged cells from thriving.…”

Section: Discussionmentioning

confidence: 99%

Azadirachta indica acts as a pro‐oxidant and modulates cell cycle associated proteins during DMBA/TPA induced skin carcinogenesis in mice

Arora

Bansal

Koul

2012

Cell Biochemistry & Function

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The present study was designed to determine the modulatory effect of aqueous Azadirachta indica leaf extract (AAILE) on cell cycle-associated proteins during two-stage skin carcinogenesis in mice. Considering the dual role of reactive oxygen species in cancer and its chemoprevention, the levels of lipid peroxidation (index of peroxidative damage) were also determined. Skin tumours were induced by topical application of 7,12-dimethylbenz(a)anthracene (DMBA) as a carcinogen followed by the repetitive application of 12-O-tetradecanoylphorbol-13-acetate (TPA) as a promoter. Skin tumours obtained in the DMBA/TPA group exhibited enhanced expression of proliferating cell nuclear antigen (PCNA, index of proliferation), p21 and cyclin D1, with no alterations in p53 expression in comparison to the control group. Tumours in AAILE + DMBA/TPA group exhibited low PCNA and cyclin D1 expression and enhanced expression of p53 and p21 in comparison to the DMBA/TPA group. The skin tumours obtained in the AAILE + DMBA/TPA group exhibited high lipid peroxidation levels in comparison to the tumours obtained in the DMBA/TPA group. The observations of the present study suggest that AAILE behaves as a pro-oxidant in the tumours, thereby rendering them susceptible to damage, which eventually culminates into its anti-neoplastic action. Also, cell cycle regulatory proteins may be modulated by AAILE and could affect the progression of cells through the cell cycle.

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Chemopreventive activity of Azadirachta indica on two‐stage skin carcinogenesis in murine model

Cited by 36 publications

References 42 publications

Anti-inflammatory polysaccharides of Azadirachta indica seed tegument

Anti-inflammatory polysaccharides of Azadirachta indica seed tegument

Protective role ofAloe veraagainst X-ray induced testicular dysfunction

Azadirachta indica acts as a pro‐oxidant and modulates cell cycle associated proteins during DMBA/TPA induced skin carcinogenesis in mice

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