Chemopreventive activity of methanol extract of <i>Melastoma malabathricum</i> leaves in DMBA-induced mouse skin carcinogenesis

Abstract

Background: Melastoma malabathricum L. Smith (family Melastomaceae) is a shrub that has been used by the Malay practitioners of traditional medicine to treat various types of ailments. The present study aimed to determine the chemopreventive activity of methanol extract of M. malabathricum leaves (MEMM) using the standard 7,12-dimethylbenz(α)anthracene (DMBA)/croton oil-induced mouse skin carcinogenesis model. Materials and Methods:In the initiation phase, the mice received a single dose of 100µl/100 µg DMBA (group I-V) or 100µl acetone (group VI) topically on the dorsal shaved skin area followed by the promotion phase involving treatment with the respective test solutions (100 μl of acetone, 10 mg/kg curcumin or MEMM (30, 100 and 300mg/kg)) for 30 min followed by the topical application of tumour promoter (100µl croton oil). Tumors were examined weekly and the experiment lasted for 15 weeks. Results: MEMM and curcumin significantly (p<0.05) reduced the tumour burden, tumour incidence and tumour volume, which were further supported by the histopathological findings. Conclusion: MEMM demonstrated chemoprevention possibly via its antioxidant and anti-inflammatory activities, and the action of flavonoids like quercitrin.

Morpho-anatomical, Molecular, and Chemical Standardization of Trillium govanianum Wall. ex D. Don: An Endangered Medicinal Herb Native to the Himalayas

“…55 However, the matK region has not been considered ideal in many plant groups due to its low PCR success rate and sequencing. 56 Molecular identification of T. govanianum by developing DNA barcode sequences is available in some studies. 57, 58 Osaloo and Kawano 57 observed less resolution in the rbcL marker-based tree in the phylogenetic analysis of Trillium and other allied species.…”

Anticarcinogenic activity of methanol extract of Melastoma malabathricum leaves is attributed to the presence of phenolics compounds and the activation of endogenous antioxidant system

“…With regards to the methanol extract of M. malabathricum leaves (MEMM), pharmacological activities such as antiproliferative, antioxidant and antiulcer have been cited by Joffry et al (2012). After 2012, several new studies have demonstrated the potential of M. malabathrium to exert antioxidant and hepatoprotective (Mamat et al, 2013), antidiabetic, antioxidant and anti-hyperlipidemic (Kumar et al, 2013), chemopreventive (Kooi et al, 2014), anticoagulant (Khoo et al, 2014), gastroprotective (Zakaria et al, 2015), anti-obesity (Karupiah & Ismail, 2015), antinociceptive (Jaios et al, 2016), anti-inflammation (Kumar et al, 2016), and chemomodulatory (Verma et al, 2016).…”