Chemoprotective effect of plant phenolics against anthracycline‐induced toxicity on rat cardiomyocytes Part II. caffeic, chlorogenic and rosmarinic acids

Abstract

The chemoprotective effects of caffeic (CA), chlorogenic (CHA) and rosmarinic (RA) acids were tested against the toxicity of doxorubicin (DOX) in neonatal rat cardiomyocytes and the iron-dependent DOX induced lipid peroxidation of heart membranes, mitochondria and microsomes. The protectivity of these acids was compared with dexrazoxan, used as an adjuvant during DOX chemotherapy. The cytoprotective effects were assessed by enzyme (LDH and ASAT) and troponin I leakage, secondly by intracellular ATP content. All hydroxycinnamic acids proved non-cytotoxic, and they stabilized both membranes and the energetic status of cardiomyocytes. After preincubation of cardiomyocytes with the test compounds (100, 200 microm; 1 h) the cardiomyocytes were treated with the toxic agent, DOX (100 microm; 8 h). The test compounds protected cardiomyocytes against DOX induced oxidative stress (RA > CHA > or = CA) on all monitored parameters. Substantial preservation of monolayer integrity of the cardiomyocytes by test compounds was also found microscopically. All the acids were more effective in the assays used than dexrazoxan. RA showed the most effective cytoprotectivity. All the acids significantly reduced the iron-dependent DOX induced lipid peroxidation of heart membranes, although of the test compounds, CHA was found to be the most effective (IC(50) = 8.04 +/- 0.74/6.87 +/- 0.52 micro m for microsomes/mitochondria).

The Preventive Effects of Chlorogenic Acid Against to Testicular Damage Caused by Methotrexate

“…29 ChA yan lız ser best ra dikal le rin sü pü rül me sin de de ğil, ay nı za man da ağır me tal iyon la rıy la şelas yon yap ma özel li ği ile de mü kem mel an ti ok si da tif et ki gös te rir. 2,29,32 Hid rojen pe rok sit; de mir ve ba kır gi bi me tal le rin var lı -ğın da, li pid pe rok si das yo nu nu te tik le yen hid rok sil ra di kal le ri ne dö nü şür. ChA ser best ra di kal olu şu -mun da önem li rol oy na yan de mir, ba kır, man gan, mo lib den gi bi ge çiş me tal le ri ile şelas yon ya pıp serbest ra di kal olu şu mu nu en gel le ye rek, mem bran yapı la rı nı li pid pe rok si das yo nun dan ko ru muş ola bi lir.…”

“…ChA 'in fe no lik asit gru bun daki di ğer fe nol le re gö re an ti-li po pe rok si da tif et ki sinin da ha güç lü ol du ğu nu gös ter miş tir. 32 Çün kü bu fe nol ler, li pid ra di kal le ri ne hız lı ca H + ve re rek, lipid pe rok si das yon zin cir re ak si yo nu nu son lan dı rır. Üs te lik re ak si yon so nu cun da olu şan fe nok si ra dika li, ye ni bir ser best ra di kal oluş tur maz.…”

Antioxidative and Cardioprotective Effects of Phyllanthus urinaria L. on Doxorubicin-Induced Cardiotoxicity

“…The cytoprotective effects of antioxidants against DOX is usually associated with the inhibition of lipid peroxidation, regardless of the type and structure of antioxidant. 16,[42][43][44][45] Although the active antioxidants in PU extract have not been identified, its suppression of lipid peroxide formation warrants further investigation.…”

Production of biologically active phenolic acids in Aronia melanocarpa (Michx.) Elliott in vitro cultures cultivated on different variants of the Murashige and Skoog medium

“…More recent studies have also revealed significant antioxidant, anti-free radical, immunostimulating and anticancer activities of some representatives of this group, e.g. protocatechuic acid, caffeic acid and chlorogenic acid (Chlopčikova et al 2004;Jiang et al 2000;Kono et al 1995;Olthof et al 2001). Hydroxybenzoic acids possess many other very important biological properties.…”