Production of biologically active phenolic acids in Aronia melanocarpa (Michx.) Elliott in vitro cultures cultivated on different variants of the Murashige and Skoog medium

Abstract

Phenolic acids, both benzoic and cinnamic acid derivatives, are plant metabolites with high therapeutic and cosmetic values. Methanolic extracts from the biomass of shoot and callus cultures of Aronia melanocarpa growing on seven variants of the Murashige and Skoog (MS) medium with different concentrations of plant growth regulators, BA and NAA, ranging from 0.1 to 3.0 mg l -1 , were examined for the production of free phenolic acids and cinnamic acid using the high-performance liquid chromatography (HPLC) method. The extracts from the shoot and callus cultures were confirmed to contain five of the twelve compounds tested for: caffeic, p-coumaric, p-hydroxybenzoic, syringic and vanillic acids. The shoot extracts contained additionally salicylic acid. Both the total amounts and the amounts of individual compounds in either the shoot or callus extracts were dependent on the concentration of cytokinin and auxin in the MS medium variants. The total amounts in the shoot and callus cultures were in the range from 93.52 to 217.00 mg 100 g -1 DW and from 47.11 to 83.83 mg 100 g -1 DW, respectively. The amounts of individual compounds showed wide variation, from 1.31 to 91.86 mg 100 g -1 DW in the shoot extracts, and from 2.58 to 40.16 mg 100 g -1 DW in the callus extracts. Salicylic acid (max. 91.86 mg 100 g -1 DW), p-coumaric acid (max. 62.39 mg 100 g -1 DW) and p-hydroxybenzoic acid (max. 50.66 mg 100 g -1 DW) dominated in the shoot extracts, while syringic acid (max. 40.16 mg 100 g -1 DW) and p-hydroxybenzoic acid (max. 23.59 mg 100 g -1 DW) were the main metabolites in the callus extracts. This is the first report on the quantitative analysis of benzoic and cinnamic acid derivatives in shoot and callus cultures of A. melanocarpa growing on MS-based media with different concentrations of selected plant growth regulators-BA and NAA. The obtained maximum amounts of some metabolites are of interest from a practical perspective.

High production of bioactive depsides in shoot and callus cultures of Aronia arbutifolia and Aronia × prunifolia

“…What was interesting, we did not observe the capacity for organogenesis in the callus lines grown on the media with different amounts of BA and NAA. The range of the tested GR amounts (0.1-3 mg/L) was comparable with the range tested by us in the experiment with shoot and callus in vitro cultures of A. melanocarpa Szopa and Ekiert 2014). The aim of the present study was to compare the biosynthetic potential of three aronias.…”

“…The aim of the present study was to compare the biosynthetic potential of three aronias. In our earlier experiments with A. melanocarpa shoot and callus cultures maintained on LS and MS media variants, some differences in the morphology of tissues had also been observed, but they stayed as separately lines-shoot and callus lines Szopa and Ekiert 2014). In contrast, in our experiments with Hypericum perforatum cvs.…”

“…Our previous investigations on A. melanocarpa shoot and callus cultures had demonstrated a high biosynthetic capacity of the cells directed towards the production of free PA, in particular hydroxybenzoic acids and cinnamic acid derivatives Szopa and Ekiert 2014). It seemed interesting to explore the biosynthetic potential of the shoot and callus cultures of A. arbutifolia and A.…”

“…We had proven that MSbased media were more conducive to the accumulation of free PA Szopa and Ekiert 2014).…”

Effect of plant growth regulators on the accumulation of indolizidine alkaloids in Securinega suffruticosa callus cultures

“…Given that the biosynthesis of alkaloids in plant cell cultures is strongly affected by both the type and concentration of PGRs used (Verpoorte et al 1991), modifications of phytohormone composition can be considered a simple, yet effective tool for improving the productivity of an in vitro system. However, contrary to other alkaloid groups such as indole (Verpoorte et al 1991;van der Heijden et al 2004;Pietrosiuk et al 2007) and diterpene derivatives (Sabater-Jara et al 2010;Malik et al 2011), reports concerning the influence of exogenous PGRs on indolizidine alkaloid accumulation are rather scarce and limited to the most commonly used phytohormones like 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA), and kinetin (KIN) (Wysokińska 1979;Ide 1991;Yuan et al 2007).…”