Effect of plant growth regulators on the accumulation of indolizidine alkaloids in Securinega suffruticosa callus cultures

Abstract

In the presented work, plant growth regulators (PGRs) were tested for their influence on biomass growth and accumulation of therapeutically-relevant indolizidine alkaloids in Securinega suffruticosa (Phyllanthaceae) callus cultures. The study included 9 auxins [2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid, indole-3-acetic acid (IAA), 4-chlorophenoxyacetic acid, indole-3-butyric acid, 4-amino-3,5,6-trichloropicolinic acid, b-naphthoxyacetic acid, 1-naphthaleneacetic acid, indole-3-propionic acid, one polar auxin transport inhibitor (2,3,5-triiodobenzoic acid) and 7 cytokinins [2-isopentenyladenine, 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea, N-(2-chloro-4-pyridinyl)-N 0 -phenylurea, diphenylurea, zeatin, kinetin (KIN), 6-benzylaminopurine]. The respective PGRs were applied at 0.5 and 5.0 mg l -1 , together with 5.0 or 0.5 mg l -1 KIN or 2,4-D (for auxins and cytokinins, respectively). The calli subjected to different phytohormone combinations (39 modifications in total) were evaluated for growth and alkaloid content, and the results tested for statistical differences. The highest concentrations of securinine (1.73 mg g -1 DW) and allosecurinine (3.11 mg g -1 DW) were recorded in the callus grown in the presence of 0.5 mg L -1 IAA and 5.0 mg L -1 KIN. To the best of the authors' knowledge, this is the first such comprehensive report concerning the influence of PGRs on alkaloid accumulation in plant in vitro cultures.

Enhanced Production of Anthraquinones and Phenolic Compounds and Biological Activities in the Cell Suspension Cultures of Polygonum multiflorum

“…The callus cultures were successfully established from the root explants obtained from in vitro seedlings of P. multiflorum . Previous studies have demonstrated that auxins and cytokinins directly influence the biosynthesis of secondary metabolites in plant cell or tissue culture [18,19]. Appropriate concentration of the medium is a critical determinant for controlling callus growth and metabolite production.…”

Terpenoid and flavonoid spectrum of in vitro cultures of Glycyrrhiza glabra revealed high chemical heterogeneity: platform to understand biosynthesis

“…Basal medium and plant growth regulators (PGRs) play a significant role in tissue morphogenesis, differentiation and secondary metabolite production (Raj et al 2015). As most of the terpenoids and flavonoids were found in the licorice roots, the role of basal media composition and PGR effect on root tissue differentiation and secondary metabolite production was investigated in in vitro raised cultures of G. glabra.…”

“…The varied chemical spectrum is probably due to PGR effect as the root explants were of same age and origin and were grown under similar in vitro conditions. Recent works of on Anacyclus pyrethrum (Singh et al 2015) and Securinega suffruticose (Raj et al 2015) have demonstrated the effect of PGR combinations on plant differentiation and secondary metabolite accumulation.…”

Influence of plant growth regulators on the shoot culture of Phyllanthus glaucus and accumulation of indolizidine alkaloids with evaluation of antimicrobial activity

“…Scientific data regarding securinega-type alkaloids in Phyllanthus species are limited and mostly relate to isolation methods (Babady et al 1996;Houghton et al 1996;Negi and Fakhir 1988). In the literature, there are described research concerning the influence of plant growth regulators on the accumulation of securinega-type alkaloids in in vitro conditions; however, none of these concern Phyllanthus species ( Raj et al 2015;Yuan et al 2007). In addition, the method for micropropagation of Phyllanthus species as a source of securinega-type alkaloids has not been provided yet.…”