Chemoprotective effects of butanol fraction of Buchholzia coriacea (Capparidaceae) against type 2 diabetes and oxidative stress in male Wistar rats

Abstract

Recent studies have shown that Type 2 diabetes (T2D) in rats can result through a synergy that link obesity to insulin resistance and β-cell dysfunction. This present study achieved T2D via high fructose (20%, p.o.), streptozotocin single dose (40 mg/kg, i.p.) (HFSTZ) in rats. Also, chemoprotective potential of butanol fraction of (BFBC) was demonstrated. Control normal and diabetic untreated (HFSTZ-induced T2D) rats received carboxymethyl cellulose (1 mg/kg, p.o.). Diabetic rats received intragastric BFBC (20, 200, 400 mg/kg), glibenclamide (0.07 mg/kg), and BFBC (200mg/kg) plus glibenclamide treatments respectively. 2,2-Diphenyl-1-picrylhydrazyl, nitric oxide radical, hydroxyl radical scavenging activities and α-amylase inhibition were assessed. After two weeks of treatments, blood glucose levels, lipid profiles, renal and liver function, serum insulin as well as oxidative stress biomarkers were assessed. BFBC shows highest antioxidants and α-amylase inhibitory activities HFSTZ-induced T2D produced hyperglycemia (p< 0.05 - 0.001; F = 5.26 - 26.47), serum hyperinsulinaemia (6-folds) plus elevated lipid peroxidation levels. Similarly, there were altered lipid profiles, liver and renal biomarker enzymes plus weight loss. BFBC administration alone or in combination with glibenclamide reversed T2D symptomatologies in treated animals, and improved body weights against control diabetic rats. antioxidant activities also improved while histological sections in treated rats show reduced tissue damage in pancreas, kidneys, liver and heart respectively. Oleic, stearic, 2-methyl-pyrrolidine-2-carboxylic and n-hexadecanoic acids were present in BFBC in large quantities given gas chromatography-mass spectrometry analysis. Overall, data from this study suggest chemoprotective potentials of BFBC against HFSTZ-induced T2D rats.

In vitro Antioxidant Activities of Hydroethanolic Extract of Defatted Wonderful Kola (Buchholzia coriacea) Seeds and Its Safety Evaluation in Murine Models

“…Our data show that HEBCS possess a good antiradical activity against DPPH, ABTS and OH radicals. This is in agreement with those reported earlier for aqueous extract (11) and methanol extract [31] of B. coriacea. The observed antioxidant activity of HEBCS in terms of DPPH, ABTS and OH scavenging potentials may be related to the high quantity of phenols and flavonoids it contains.…”

Ameliorative effects of Buchholzia coriacea seed on aluminium chloride induced cytotoxicity in the liver of adult male wistar rats

“…13,15 The aqueous seed extract of Buchholzia coriacea may have attenuated the AlCl 3 induced increase in liver homogenate concentrations of MDA due to its antioxidant potential. 14,17 In this present study elevated levels of malondialdehyde (MDA) but reduced levels of glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) in the liver samples of aluminum chloride (AlCl 3 ) -treated rats was recorded. The significant decrease recorded in the activities of liver antioxidant protein and enzymes (GSH, SOD and CAT) reflect the failure of antioxidant defense system to overcome the influx of ROS precipitated by AlCl 3 administration.…”