Chemotaxis as an Expression of Communication of Tetrahymena

Kőhidai, László

doi:10.1007/978-3-319-32211-7_5

Cited by 3 publications

(4 citation statements)

References 49 publications

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“…In such interactions, cells producing higher levels of malate may secrete it into the surrounding medium, while cells producing lower levels may utilize the excreted malate. Although chemotaxis towards amino acids and peptides has been well-studied in Tetrahymena , research on chemotaxis towards dicarboxylic acids, including malate, is still forthcoming [ 34 ]. However, in Pseudomonas fluorescence , chemotaxis towards dicarboxylic acids including malate, succinate, and fumarate has been observed, suggesting these metabolites function as cell-to-cell signaling molecules [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

Revealing gene expression heterogeneity in a clonal population of Tetrahymena thermophila through single-cell RNA sequencing

Kojima,

Kashiwagi,

Ikegami

2024

Biochemistry and Biophysics Reports

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Section: Resultsmentioning

confidence: 99%

Revealing gene expression heterogeneity in a clonal population of Tetrahymena thermophila through single-cell RNA sequencing

Kojima,

Kashiwagi,

Ikegami

2024

Biochemistry and Biophysics Reports

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“…In such interactions, cells producing higher levels of malate may secrete it into the surrounding medium, while cells producing lower levels may utilize the excreted malate. Although chemotaxis towards amino acids and peptides is well-studied in Tetrahymena , research on chemotaxis towards dicarboxylic acids, including malate, is still forthcoming (Köhidai, 2016). However, in Pseudomonas fluorescence , chemotaxis towards dicarboxylic acids including malate, succinate, and fumarate has been observed, suggesting these metabolites function as cell-to-cell signaling molecules (Oku, 2012).…”

Section: Resultsmentioning

confidence: 99%

Section: Regressing Out the Cell Cycle Scoresmentioning

confidence: 99%

Revealing Gene Expression Heterogeneity in a Clonal Population ofTetrahymena thermophilathrough Single-Cell RNA Sequencing

Kojima,

Kashiwagi,

Ikegami

2023

Preprint

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We investigated the gene expression variability in a population of 5,000Tetrahymena thermophilacells using single-cell RNA sequencing. During preprocessing, we enhanced read mappings by estimating the 3′-untranslated regions using the “peaks2utr” method. The resulting expression patterns enabled us to identify several prevalent clusters within the RNA expression space. Some of these clusters corresponded to specific cell phases. After regressing out these phase-related clusters, we performed a gene ontology analysis to identify the remaining clusters. We propose that these remaining clusters represent emerging sub-populations within theTetrahymenapopulation, primarily associated with translation, energy production, and cell structure.

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“…Comparative to survival bioassays, behavioral effects are considered to be the most sensitive indicators of environmental changes because they occur quickly and can be detected at low doses and in non-invasive ways on living organisms [ 27 ]. In Tetrahymena , behavior studies mainly focus on phagocytosis activity [ 28 ], chemotaxis response [ 29 ], and cell motility [ 30 ]. Among them, cell locomotion is easy to monitor to directly manifest the ciliary motility.…”

Section: Introductionmentioning

confidence: 99%

Establishing a High-Throughput Locomotion Tracking Method for Multiple Biological Assessments in Tetrahymena

Suryanto

Vasquez

Roldan

et al. 2022

Cells

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Protozoa are eukaryotic, unicellular microorganisms that have an important ecological role, are easy to handle, and grow rapidly, which makes them suitable for ecotoxicity assessment. Previous methods for locomotion tracking in protozoa are largely based on software with the drawback of high cost and/or low operation throughput. This study aimed to develop an automated pipeline to measure the locomotion activity of the ciliated protozoan Tetrahymena thermophila using a machine learning-based software, TRex, to conduct tracking. Behavioral endpoints, including the total distance, velocity, burst movement, angular velocity, meandering, and rotation movement, were derived from the coordinates of individual cells. To validate the utility, we measured the locomotor activity in either the knockout mutant of the dynein subunit DYH7 or under starvation. Significant reduction of locomotion and alteration of behavior was detected in either the dynein mutant or in the starvation condition. We also analyzed how Tetrahymena locomotion was affected by the exposure to copper sulfate and showed that our method indeed can be used to conduct a toxicity assessment in a high-throughput manner. Finally, we performed a principal component analysis and hierarchy clustering to demonstrate that our analysis could potentially differentiate altered behaviors affected by different factors. Taken together, this study offers a robust methodology for Tetrahymena locomotion tracking in a high-throughput manner for the first time.

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Chemotaxis as an Expression of Communication of Tetrahymena

Cited by 3 publications

References 49 publications

Revealing gene expression heterogeneity in a clonal population of Tetrahymena thermophila through single-cell RNA sequencing

Revealing gene expression heterogeneity in a clonal population of Tetrahymena thermophila through single-cell RNA sequencing

Revealing Gene Expression Heterogeneity in a Clonal Population ofTetrahymena thermophilathrough Single-Cell RNA Sequencing

Establishing a High-Throughput Locomotion Tracking Method for Multiple Biological Assessments in Tetrahymena

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