Chemotaxis in Pseudomonas aeruginosa

Moench, Thomas T.; Konetzka, Walter A.

doi:10.1128/jb.133.1.427-429.1978

Cited by 32 publications

(14 citation statements)

References 2 publications

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“…The concentration-dependent chemotactic response toward AGN in capillaries shown in Fig. 2 resembles those demonstrated for other amino acids with P. aeruginosa (6,19,20). Thus, chemotaxis increased with concentration to 10 mM AGN before steadily declining to levels less than the background.…”

Section: Identification Of Agn As a Nutrient And Chemoattractantsupporting

confidence: 62%

L-Arogenate Is a Chemoattractant Which Can Be Utilized as the Sole Source of Carbon and Nitrogen by Pseudomonas aeruginosa

Fischer¹,

Jiang²,

Gu³

et al. 1997

Appl Environ Microbiol

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L-Arogenate is a commonplace amino acid in nature in consideration of its role as a ubiquitous precursor of L-phenylalanine and/or L-tyrosine. However, the questions of whether it serves as a chemoattractant molecule and whether it can serve as a substrate for catabolism have never been studied. We found that Pseudomonas aeruginosa recognizes L-arogenate as a chemoattractant molecule which can be utilized as a source of both carbon and nitrogen. Mutants lacking expression of either cyclohexadienyl dehydratase or phenylalanine hydroxylase exhibited highly reduced growth rates when utilizing L-arogenate as a nitrogen source. Utilization of L-arogenate as a source of either carbon or nitrogen was dependent upon 54 , as revealed by the use of an rpoN null mutant. The evidence suggests that catabolism of L-arogenate proceeds via alternative pathways which converge at 4-hydroxyphenylpyruvate. In one pathway, prephenate formed in the periplasm by deamination of L-arogenate is converted to 4-hydroxyphenylpyruvate by cyclohexadienyl dehydrogenase. The second route depends upon the sequential action of periplasmic cyclohexadienyl dehydratase, phenylalanine hydroxylase, and aromatic aminotransferase.

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Section: Identification Of Agn As a Nutrient And Chemoattractantsupporting

confidence: 62%

L-Arogenate Is a Chemoattractant Which Can Be Utilized as the Sole Source of Carbon and Nitrogen by Pseudomonas aeruginosa

Fischer¹,

Jiang²,

Gu³

et al. 1997

Appl Environ Microbiol

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“…Nmax of P. aeruginosa P1525 in tap water supplied with different amounts (,ug of C per liter) of either acetate (0) or aspartate (0). sponses of P. aeruginosa have been observed with amino acids of which arginine, with a threshold of 0.7 ,uM, was the best attractant (18,19). Citrulline and ornithine, intermediates of the degradation of arginine by the arginine deiminase pathway in P. aeruginosa, did not favor growth of strain P1525 when present at a low concentration (Fig.…”

Section: Discussionmentioning

confidence: 90%

Growth of Pseudomonas aeruginosa in tap water in relation to utilization of substrates at concentrations of a few micrograms per liter

Kooij¹,

Oranje²,

Hijnen³

1982

Appl Environ Microbiol

101

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Five Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particular strain (P1525) isolated from drinking water. This strain was tested for the utilization of 30 compounds supplied at a concentration of 25 microgram of C per liter. The growth rate (number of generations per hour) of strain P1525 in this tap water was approximately 0.005 h-1, and with 10 compounds it was larger than 0.03 h-1. An average yield of 6.2 x 10(9) colony-forming units per mg of C was obtained from the maximum colony counts (colony-forming units per milliliter). The average yield and maximum colony count of strain P1525 grown in tap water supplied with a mixture of 45 compounds, each at a concentration of 1 microgram of C per liter, enabled us to calculate that 28 compounds were utilized. Growth rates of two P. aeruginosa strains (including P1525) in various types of water at 15 degrees C were half of those of a fluorescent pseudomonad. The concentrations of assimilable organic carbon calculated from maximum colony counts and average yield values amounted to 0.1 to 0.7% of the total organic carbon concentrations in five types of tap water. The assimilable organic carbon percentages were about 10 times larger in river water and in water after ozonation.

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“…E. coli and Salmonella sp., for example, are attracted to many kinds of sugars and to some amino acids (2, 13). Similarly, Pseudomonas aeruginosa responds favorably to an array of organic acids, compounds that this species favors as growth substrates (14,15).…”

mentioning

confidence: 99%

Aromatic acids are chemoattractants for Pseudomonas putida

Harwood¹,

Rivelli²,

Ornston³

1984

J Bacteriol

111

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A quantitative capillary assay was used to show that aromatic acids, compounds that are chemorepellents for Escherichia coli and Salmonella sp., are chemoattractants for Pseudomonas putida PRS2000. The most effective attractants were benzoate; p-hydroxybenzoate; the methylbenzoates; m-, p-, and o-toluate; salicylate; DLmandelate; ,-phenylpyruvate; and benzoylformate. The chemotactic responses to these compounds were inducible. Taxis to benzoate and m-toluate was induced by l-ketoadipate, a metabolic intermediate formed

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Chemotaxis in Pseudomonas aeruginosa

Abstract: A chemotaxis system for Pseudomonas aeruginosa was defined by using the method of Adler. Cells were attracted to compounds in the order ammonium chloride > amino acids > organic acids. Two sugars were assayed and elicited no response. Comparisons with other model systems are discussed.

Cited by 32 publications

References 2 publications

L-Arogenate Is a Chemoattractant Which Can Be Utilized as the Sole Source of Carbon and Nitrogen by Pseudomonas aeruginosa

L-Arogenate Is a Chemoattractant Which Can Be Utilized as the Sole Source of Carbon and Nitrogen by Pseudomonas aeruginosa

Growth of Pseudomonas aeruginosa in tap water in relation to utilization of substrates at concentrations of a few micrograms per liter

Aromatic acids are chemoattractants for Pseudomonas putida

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