Chemotherapeutic potential of quercetin on human bladder cancer cells

Abstract

In an effort to improve local bladder cancer control, we investigated the cytotoxic and genotoxic effects of quercetin on human bladder cancer T24 cells. The cytotoxic effect of quercetin against T24 cells was examined by MTT test, clonogenic assay as well as DNA damaging effect by comet assay. In addition, the cytotoxic effect of quercetin on the primary culture of papillary urothelial carcinoma (PUC), histopathological stage T1 of low- or high-grade tumours, was investigated. Our analysis demonstrated a high correlation between reduced number of colony and cell viability and an increase in DNA damage of T24 cells incubated with quercetin at doses of 1 and 50 µM during short term incubation (2 h). At all exposure times (24, 48 and 72 h), the efficacy of quercetin, administered at a 10× higher dose compared to T24 cells, was statistically significant (P < 0.05) for the primary culture of PUC. In conclusion, our study suggests that quercetin could inhibit cell proliferation and colony formation of human bladder cancer cells by inducing DNA damage and that quercetin may be an effective chemopreventive and chemotherapeutic agent for papillary urothelial bladder cancer after transurethral resection.

Actein induces autophagy and apoptosis in human bladder cancer by potentiating ROS/JNK and inhibiting AKT pathways

“…Bladder cancer is a highly prevalent tumor and is related to substantial morbidity, mortality and cost [ 1 , 2 , 49 ]. The environmental or occupational exposures to carcinogens, particularly tobacco, are the major risk factors for promoting the bladder cancer [ 50 , 51 ].…”

“…Human bladder cancer is one of the most common genitourinary malignant cancers, leading to health issue worldwide [ 1 , 2 ]. Presently, the existed therapeutic strategies, including radical cystectomy and chemotherapy, are far from to be satisfied considering the metastases and recurrence of human bladder cancer [ 3 – 5 ].…”

“…Human bladder cancer is one of the most common genitourinary malignant cancers, leading to health issue worldwide [ 1 , 2 ]. Presently, the existed therapeutic strategies, including radical cystectomy and chemotherapy, are far from to be satisfied considering the metastases and recurrence of human bladder cancer [ 3 – 5 ]. Thus, although advances have been made in radiotherapy, perioperative chemotherapy, and surgical technology, the 10-year survival rate remains grave after radical cystectomy [ 3 , 6 ].…”

“…Presently, the existed therapeutic strategies, including radical cystectomy and chemotherapy, are far from to be satisfied considering the metastases and recurrence of human bladder cancer [ 3 – 5 ]. Thus, although advances have been made in radiotherapy, perioperative chemotherapy, and surgical technology, the 10-year survival rate remains grave after radical cystectomy [ 3 , 6 ]. Hence, it is still urgently to find and develop new and effective strategies against bladder cancer.…”

Nutraceuticals: Curative Integrative Cancer Treatment

“…Kaempferol, luteolin, and quercetin have shown dose dependent reduction in OVCAR3 ovarian cancer growth and VEGF production [25]. Quercetin is cytotoxic to papillary urothelial carcinoma T24 cells, p<0.05 [57]. M. buchananii quercetin has an EC50 of 1.38 μg/ml and a TEAC of 89.69 μg/ml.…”