Chemotherapeutic Targeting of Cell Death Pathways

Mansilla, Sylvia; Llovera, Laia; Portugal, José

doi:10.2174/187152012800228805

Cited by 45 publications

(35 citation statements)

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“…It has been reported that acute and chronic exposure to CP results in elevated apoptotic germ cell rates [5,24,25]. A caspase-dependent pathway could be triggered by chemotherapeutics [26].…”

Section: Discussionmentioning

confidence: 99%

Anti-oxidative and anti-apoptotic roles of spermatogonial stem cells in reversing cisplatin-induced testicular toxicity

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Anti-oxidative and anti-apoptotic roles of spermatogonial stem cells in reversing cisplatin-induced testicular toxicity

et al. 2015

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“…The efficacy of chemotherapeutic agents is largely dependent on their ability to inhibit the growth of tumor cells (1). A number of studies have demonstrated that certain phytochemicals present in medicinal herbs exert antitumor activity by inhibiting cancer cell growth (2).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells

Huang

Zou

2015

Experimental and Therapeutic Medicine

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Abstract. The steroidal saponin TTB2 can be isolated from the n-BuOH extracts of Trillium tschonoskii Maxim. The aim of the present study was to observe whether this saponin exerted any cytotoxic effects on malignant sarcoma cells, and to further investigate the possible underlying molecular mechanisms. The cell viability, cell cycle arrest and phosphorylation of certain important signal molecules in the sarcoma cell line were investigated. It was found that TTB2 inhibited the growth of the Ewing sarcoma cell line and arrested cells in the G 2 /M and S phases of the cell cycle in a dose-and time-dependent manner. Furthermore, the phosphorylation of extracellular signal-regulated kinase was inhibited by TTB2. In conclusion, the results showed that the saponin TTB2 isolated from T. tschonoskii Maxim exerts anticancer effects and may be a potential candidate for the development of anticancer drugs for use in the treatment of cancer.

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“…During the past decade, accumulating evidence has suggested that many cancer therapeutic agents induce apoptosis. Although the anticancer agent mechanisms of the apoptotic pathway are controversial, the killing of cancer cells through the induction of apoptosis has been recognized as a novel strategy for identifying chemotherapeutic as well as chemopreventive agents (10)(11)(12).…”

Section: Induction Of Apoptosis In Mda-mb-231 Human Breast Carcinoma mentioning

confidence: 99%

Induction of apoptosis in MDA-MB-231 human breast carcinoma cells with an ethanol extract of Cyperus rotundus L. by activating caspases

et al. 2014

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Abstract. Cyperus rotundus L. belongs to the Cyperaceae family and is a well documented traditional medicinal herb. Its rhizome has been reported to possess wide spectrum pharmacological activities including anti-inflammatory and antioxidant activity. However, the cellular and molecular mechanisms of the anticancer activity have not been elucidated. In the present study, we investigated the pro-apoptotic effects of C. rotundu rhizomes in a human breast carcinoma MDA-MB-231 cell model. Treatment of MDA-MB-231 cells with an ethanol extract of C. rotundu rhizomes (EECR) and a methanol extract of C. rotundu rhizomes (MECR), but not a water extract of C. rotundu rhizomes, resulted in potent antiproliferative activity. The activity of the EECR was higher than that of the MECR and was associated with the induction of apoptosis. The induction of apoptosis by the EECR was associated with upregulation of death receptor 4 (DR4), DR5 and pro-apoptotic Bax, as well as downregulation of anti-apoptotic survivin and Bcl-2. EECR treatment also downregulated Bid expression and activated caspase-8 and -9, the respective initiator caspases of the extrinsic and intrinsic apoptotic pathways. The increase in mitochondrial membrane depolarization was correlated with activation of effector caspase-3 and cleavage of poly(ADP-ribose) polymerase, a vital substrate of activated caspase-3. Blockage of caspase activation by pretreatment with a pan-caspase inhibitor consistently inhibited apoptosis and abrogated growth inhibition in EECR-treated MDA-MB-231 cells. Although reactive oxygen species (ROS) increased following treatment with the EECR, inhibiting ROS with a ROS scavenger did not attenuate EECR-induced apoptosis. Furthermore, inhibitors of phosphatidylinositol 3-kinase (PI3K)/Akt and mitogen-activated protein kinase (MAPK) signaling pathways failed to reverse EECR-induced apoptosis and growth inhibition. These results suggest that the pro-apoptotic activity of the EECR may be regulated by a caspase-dependent cascade through activation of both intrinsic and extrinsic signaling pathways that is not associated with ROS generation or the PI3K/Akt and MAPK pathways.

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Chemotherapeutic Targeting of Cell Death Pathways

Cited by 45 publications

References 0 publications

Anti-oxidative and anti-apoptotic roles of spermatogonial stem cells in reversing cisplatin-induced testicular toxicity

Anti-oxidative and anti-apoptotic roles of spermatogonial stem cells in reversing cisplatin-induced testicular toxicity

Cytotoxicity of the saponin TTB2 on Ewing sarcoma cells

Induction of apoptosis in MDA-MB-231 human breast carcinoma cells with an ethanol extract of Cyperus rotundus L. by activating caspases

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