CheV1 Plays an Important Role in Chemotaxis of Vibrio Cholerae

Abstract

Abstract-The complete genome sequence of V. cholerae has revealed the presence of four CheV's, namely putative CheV, CheV1, CheV2 and CheV3, along with the three chemotaxis-related signaling systems designated as System I, II and III. To determine the roles of the CheV proteins, whose genes are located outside of the che gene clusters, we examined their cellular localization and then their roles in chemotaxis. Among the four CheV's, CheV1 plays a major role in chemotaxis as observed with swarm assay and fluorescence microscopy, suggesting its involvement in System II.

Hypoxia‐induced localization of chemotaxis‐related signaling proteins in Vibrio cholerae

“…V. cholerae has at least four cheV homologs: cheV1 (VC0395_A1207), cheV2 (VC0395_A1592), cheV3 (VC0395_1794) and putative cheV (VC0395_0285). Among them, we have assigned cheV1 to System II as overexpression of the gene interferes with swarming (Hiremath et al, 2013) and localization of CheA2(II)-GFP (data not shown). On the other hand, overexpression of any cheV homolog did not drastically disturb hypoxia-induced localization of CheA1(I)-GFP (data not shown).…”

The chemosensory systems of Vibrio cholerae

“…An earlier study using swarm assays and fluorescence microscopy discovered that this CheV plays a role in chemotaxis (Hiremath et al ., 2013).…”

“…(It is noteworthy that in this specific study cheV genes are numbered from a silent 0 instead of explicit 1: CheV(VC1602), CheV1(VC2006), CheV2(VC2202), and CheV3(VCA0954). So the labeling “CheV” used in the study (Hiremath et al ., 2013) actually refers to the same protein “CheV1” of the later study (Yang et al ., 2018).…”