Chicken heterophils from commercially selected and non-selected genetic lines express cytokines differently after in vitro exposure to Salmonella enteritidis

Redmond, Sarah; Chuammitri, Phongsakorn; Andreasen, Claire B.; Palić, Dušan; Lamont, Susan J.

doi:10.1016/j.vetimm.2009.05.010

Cited by 65 publications

(51 citation statements)

References 51 publications

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“…Blood was collected in Vacutainer tubes containing EDTA and mixed thoroughly. Following blood collection, heterophils were isolated using the method described by Redmond et al (35). All animal experimental protocols were approved by the institute and carried out in accordance with the guidelines for experimental animals established by the Ministry of Science and Technology (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

Flagellin from Recombinant Attenuated Salmonella enterica Serovar Typhimurium Reveals a Fundamental Role in Chicken Innate Immunity

Pan

QiuXia

Geng

et al. 2012

Clin. Vaccine Immunol.

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bRecombinant attenuated Salmonella vaccines have been extensively studied, with a focus on eliciting specific immune responses against foreign antigens. However, very little is known about the innate immune responses, particularly the role of flagellin, in the induction of innate immunity triggered by recombinant attenuated Salmonella in chickens. In the present report, we describe two Salmonella enterica serovar Typhimurium vaccine strains, wild-type (WT) or flagellin-deficient (flhD) Salmonella, both expressing the fusion protein (F) gene of Newcastle disease virus. We examined the bacterial load and spatiotemporal kinetics of expression of inflammatory cytokine, chemokine, and Toll-like receptor 5 (TLR5) genes in the cecum, spleen, liver, and heterophils following oral immunization of chickens with the two Salmonella strains. The flhD mutant exhibited an enhanced ability to establish systemic infection compared to the WT. In contrast, the WT strain induced higher levels of interleukin-1␤ (IL-1␤), CXCLi2, and TLR5 mRNAs in cecum, the spleen, and the heterophils than the flhD mutant at different times postinfection. Collectively, the present data reveal a fundamental role of flagellin in the innate immune responses induced by recombinant attenuated Salmonella vaccines in chickens that should be considered for the rational design of novel vaccines for poultry.

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Section: Methodsmentioning

confidence: 99%

Flagellin from Recombinant Attenuated Salmonella enterica Serovar Typhimurium Reveals a Fundamental Role in Chicken Innate Immunity

Pan

QiuXia

Geng

et al. 2012

Clin. Vaccine Immunol.

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“…The consequences of Salmonella infection on the expression of the different TLRs, particularly TLR4, have been studied widely using quantitative reverse transcription-PCR (qRT-PCR) in various in vitro or in vivo models. Variation of TLR4 has been analyzed in vitro after incubation of peritoneal macrophages (14), ovarian follicles (37), and thrombocytes (35) with LPS and in heterophils in response to S. Enteritidis (30,31). On the other hand, its expression has been measured during the acute phase in Salmonella-infected chickens, in whole ceca or spleens (1,19,26,27,34,36) and in peripheral blood lymphocytes (28).…”

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confidence: 99%

Expression of Toll-Like Receptor 4 and Downstream Effectors in Selected Cecal Cell Subpopulations of Chicks Resistant or Susceptible to Salmonella Carrier State

Chaussé¹,

Grépinet²,

Bottreau³

et al. 2011

Infect Immun

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Toll-like receptor 4 (TLR4), which recognizes lipopolysaccharide from Gram-negative bacteria, plays a major role in resistance of mice and humans to Salmonella infection. In chickens, Salmonella may establish a carrier state whereby bacteria are able to persist in the host organism without triggering clinical signs. Based on cellular morphological parameters, we developed a method, without using antibodies, to separate three cecal cell subpopulations: lymphocytes, enterocytes, and a population encompassing multiple cell types. We analyzed the mRNA expression of TLR4, interleukin-1␤ (IL-1␤), IL-8, IL-12, and lipopolysaccharide-induced tumor necrosis factor alpha factor (LITAF) in cecal subpopulations of chicks from inbred lines resistant or susceptible to the carrier state infected with Salmonella enterica serovar Enteritidis. The results showed that resistance to the carrier state in chicks is associated with a larger percentage of lymphocytes and with higher levels of expression of TLR4 and IL-8 at homeostasis in the three cell subpopulations, as well as with a higher level of expression of LITAF in lymphocytes during the carrier state. In contrast to the early phase of infection, the carrier state is characterized by no major cell recruitment differences between infected and noninfected animals and no significant modification in terms of TLR4, IL-1␤, IL-8, IL-12, and LITAF expression in all cell subpopulations measured. However, TLR4 expression increased in the lymphocytes of chicks from the susceptible line, reaching the same level as that in infected chicks from the resistant line. These observations suggest that the carrier state is characterized by a lack of immune activation and highlight the interest of working at the level of the cell population rather than that of the organ.

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“…However, we have noted that the basal expression in control groups differed considerably based on the type of tissue used for the analysis. In this study we were therefore interested in the basal expression of selected chicken cytokines in different organs of hens being aware of the fact that the basal expression may differ slightly in different chicken breeds (Swaggerty et al, 2004;Redmond et al, 2009). Interestingly, for three cytokines, iNOS, IL-18 and IL-6, we observed rather unexpected, tissuedependent expression profiles.…”

mentioning

confidence: 99%

Characterisation of basal expression of selected cytokines in the liver, spleen, and respiratory, reproductive and intestinal tract of hens

Kolesárová¹,

Spišáková²,

Matulová³

et al. 2011

Vet. Med. - Czech

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ABSTRACT:In this study we investigated the basal expression of chicken IFNγ, IL-18, IL-1β, LITAF, IL-12p35, IL-12p40, iNOS, IL-17, IL-15, IL-6, IL-8 and IL-22 in the ileum, caecum, colon, liver, spleen, lungs, blood and magnum of hens using real time PCR. We show that IL-18, LITAF and IL-15 were highly expressed in most tissues while IL-6 and IL-17 were usually expressed at a low level. IL-17 and IL-22 were expressed mainly in the lungs and intestinal tract and both subunits of IL-12 were produced in the liver. Unusually high expression of iNOS was observed in the blood which may explain the rapid increase in iNOS at inflamed sites in chickens. The second unexpected expression profile was that of IL-18 and IL-6 in magnum. These two cytokines were highly expressed in magnum in the absence of any infection pointing to an important, though as yet uncharacterised, physiological role in the reproductive tract of hens.

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Chicken heterophils from commercially selected and non-selected genetic lines express cytokines differently after in vitro exposure to Salmonella enteritidis

Cited by 65 publications

References 51 publications

Flagellin from Recombinant Attenuated Salmonella enterica Serovar Typhimurium Reveals a Fundamental Role in Chicken Innate Immunity

Flagellin from Recombinant Attenuated Salmonella enterica Serovar Typhimurium Reveals a Fundamental Role in Chicken Innate Immunity

Expression of Toll-Like Receptor 4 and Downstream Effectors in Selected Cecal Cell Subpopulations of Chicks Resistant or Susceptible to Salmonella Carrier State

Characterisation of basal expression of selected cytokines in the liver, spleen, and respiratory, reproductive and intestinal tract of hens

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