Chicken IL-7 as a potent adjuvant enhances IBDV VP2 DNA vaccine immunogenicity and protective efficacy

Huo, Shanshan; Zuo, Yuanmei; Li, Nan; Li, Xiujin; Zhang, Yonghong; Wang, Liyue; Liu, Hao; Zhang, Jianlou; Cui, Dan; He, Peng; Xu, Jian; Li, Yan; Zhu, X. D.; Zhong, Fei

doi:10.1016/j.vetmic.2016.08.016

Cited by 20 publications

(19 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pET20b(+) plasmid, a prokaryotic expression vector, was purchased from Novagen. pcDNA-chIL-7/MH plasmid containing the full-length chicken IL-7 gene was previously constructed [ 15 , 16 ]. Mouse immature B lymphocytes (2E8 cells) for IL-7 activity assay were from ATCC (Manassas, VA).…”

Section: Methodsmentioning

confidence: 99%

“…Recombinant IBDV VP2 protein was prepared in our laboratory as described previously [ 15 , 16 ]. Mouse anti-His antibody and horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (IgG-HRP) (sc-2031) were purchased from Santa Cruz Biotechnology.…”

Section: Methodsmentioning

confidence: 99%

“…Antibody titers were measured by ELISA as previously described [ 15 , 16 ] using the recombinant IBDV VP2 protein as antigen and HRP-conjugated goat anti-chicken IgG (Sigma) as a secondary Ab, and virus neutralization (VN) assays were performed as described previously [ 15 , 16 ].…”

Section: Methodsmentioning

confidence: 99%

“…In recent years, many adjuvants for inactivated IBDV vaccine have been investigated. Besides conventional adjuvants (such as alum, liposomes, microparticles and oil/water emulsions), biological adjuvants have been investigated, mainly involving recombinant polypeptides [ 9 ], amino acids [ 10 ], active components of Chinese traditional medicine [ 11 ] and plasmid-based cytokine genes, including IL-2 [ 12 , 13 ], IL-6 [ 14 ], IL-7 [ 15 , 16 ], IL-18 [ 17 ] and IFN-γ [ 18 , 19 ] genes, to enhance IBDV VP2 DNA vaccine immunogenicity. However, recombinant proteins as the biological adjuvants (recombinant cytokines or other recombinant proteins) for inactivated IBDV vaccine have only received limited attention.…”

Section: Introductionmentioning

confidence: 99%

“…Recently we prepared recombinant chIL-7 from a eukaryotic expression system and demonstrated that it possessed antiviral activity against IBDV [ 15 , 16 ]. We also showed that a plasmid-based chIL-7 gene vector could enhance the immunogenicity of IBDV VP2 DNA vaccine [ 15 , 16 ]. However, whether recombinant chIL-7 could function as an adjuvant to enhance inactivated IBDV vaccine efficiency was not established.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Recombinant chicken interleukin-7 as a potent adjuvant increases the immunogenicity and protection of inactivated infectious bursal disease vaccine

Cui

Zhang

Zuo

et al. 2018

Vet Res

Self Cite

View full text Add to dashboard Cite

Our previous work showed that a plasmid-based chicken interleukin-7 (chIL-7) gene expression vector possessed potent adjuvant activity for a VP2 DNA vaccine against chicken infectious bursal disease virus (IBDV). Whether recombinant chIL-7 prepared in procaryotic expression system has the adjuvant activity for inactivated IBDV vaccine remains unknown. Here, we prepared recombinant chIL-7 using an E. coli expression system and analyzed its adjuvant activity for the inactivated IBDV vaccine. The results show that the recombinant chIL-7 was successfully prepared in E. coli using the pET20b vector, which possessed biological activity to stimulate mouse B lymphocyte proliferation. Co-administration of the chIL-7 with inactivated IBDV vaccine significantly increased specific serum antibody titers against IBDV, enhanced lymphocyte proliferation and IFN-γ and IL-4 productions, and increased protection against virulent IBDV infection.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%