Chilled and post‐thaw storage of sperm in different goldfish types

Abstract

The effective storage time of sperm after stripping (for 48 hr in 6-hr intervals) and after thawing (for 6 hr in 2-hr intervals) in Black moor, Oranda and Calico goldfish types was investigated. Variations in sperm density were also measured in all lines. The efficiency of a sperm cryopreservation method formerly developed for common carp was recorded in all three goldfish lines. Motility parameters ((pMOT, %), curvilinear velocity (VCL, μm/s) and straightness (STR, %)) of Black moor sperm did not decrease significantly during 48 hr of storage. A significant reduction in the Oranda type compared to the fresh control was observed in pMOT after 42 (23 ± 2%) and VCL after 36 (94 ± 12 μm/s) hours (pMOT 84 ± 5%, VCL 150 ± 11 μm/s). In the Calico type, pMOT decreased significantly already after 18 (42 ± 26%) and VCL after 6 (105 ± 8 μm/s) hours (fresh: pMOT 92 ± 5%, VCL 151 ± 6 μm/s). A high pMOT immediately following thawing was measured in Oranda (46 ± 12%) and Calico (55 ± 15%) types, whereas a reduced pMOT was recorded in Black moor (24 ± 19%). In Calico, pMOT showed a significant reduction after 6 hr (19 ± 11%) in comparison with the initial value, with no changes observed in VCL and STR. None of the parameters changed in the Black moor and Oranda types. Evidence was found that different goldfish lines have different sperm quality and characteristics. Further studies can investigate the possible effects of chilled and post-thaw storage on the fertilizing capacity of sperm in the Black moor, Oranda and Calico goldfish types.

DNA methylation stability in fish spermatozoa upon external constraint: Impact of fish hormonal stimulation and sperm cryopreservation

“…The immediate effect of cryopreservation is a reduced ability of spermatozoa to successfully fertilize eggs, as a consequence of damages induced at a cellular level, including plasma membrane alteration, mitochondria breakdown, and overall reduced sperm motility. In our cryopreservation condition, the cryoprotectant that gave the best sperm quality at thawing was methanol, in accordance with the observations in goldfish (Bernáth et al, ), or in eel and carp (Herranz‐Jusdado et al, ; Horváth, Miskolczi, & Urbányi, ). Although fertilization and development success was variable between sperm samples, global DNA methylation levels were unaffected by cryopreservation with methanol.…”

The comparison of two different extenders for the improvement of large‐scale sperm cryopreservation in common carp (Cyprinus carpio)

“…Samples were diluted in common carp generally used (“Grayling” extender: 200 mM glucose, 40 mM KCl, 30 mM Tris, pH: 8.0 ± 0.2, Horváth et al, , Bernáth et al, ) and in a new extender (“Pike” extender: 150 mM glucose, 75 mM NaCl, 30 mM KCl, 1 mM Na 2 HPO 4 ×12H 2 O, 1 mM MgCl2×6H 2 O, 1 mM CaCl 2 ×2H 2 O, 20 mM Tris, and 0.5% BSA, pH: 8.0 ± 0.2, Bernáth, Ittzés, et al, ) in all experiments at a ratio of 1:9. Methanol (10%) was added to the solution as intracellular cryoprotectant.…”

“…20 mM Tris, and 0.5% BSA, pH: 8.0 ± 0.2, Bernáth, Ittzés, et al, 2017) in all experiments at a ratio of 1:9. Methanol (10%) was added to the solution as intracellular cryoprotectant.…”

“…Cryopreservation is an assisted reproductive technique which allows the long-term storage of different tissues or cells (Bernáth, Ittzés, et al, 2017;Cabrita et al, 2010;Martínez-Páramo et al, 2017). The sperm cryopreservation in freshwater and marine fish can potentially be applied in the aquaculture sector (genetic selection programmes, broodstock management, biotechnological studies) and in the conservation biology as sperm preservation in vulnerable and protected species (Asturiano, Cabrita, & Horváth, 2017;Martínez-Páramo et al, 2017;Tiersch, Yang, & Hu, 2012).…”

Preservation of fish male germplasm in Poland

“…The great advantage of straws was possibility to print the information concerning individual fish directly on straws with the use of straw printer. The developed method involved diluting semen in the Kopeika, Lahnsteiner or "Pike" extender (at 1:6 or 1:9 ratio) (Kopeika, 1986;Lahnsteiner et al, 2000;Bernath et al, 2017).…”