Chilling Sensitivity of Cucumber Cotyledon Protoplasts and Seedlings

Mk, Pomeroy; Jb, Mudd

doi:10.1104/pp.84.3.677

Cited by 9 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In temperature-resistant regenerated sugar beet plants obtained under the conditions of the short-term action of extreme temperatures, a signifi cant increase (almost 5−7 times) in free proline content in leaf blades was found, while in the control treatment, prolin content increased only 2−3 times (Fig. 2), which is compliant with the available data on the accumulation of proline in temperature-resistant lines of other plant species (Pomeroy and Mudd, 1987;Kolupaev et al, 2014). The scientists found that free proline as a polyfunctional acid involved in complex integral processes of plant adaptation and resistance.…”

Section: Resultssupporting

confidence: 89%

Obtaining Temperature-Resistant Sugar Beet Lines (<i>Beta vulgaris</i> L.)

Klyachenko¹,

Prysiazhniuk²,

Bokiy³

et al. 2023

Ecol. Eng. Environ. Technol.

View full text Add to dashboard Cite

This study aimed to investigate the sugar beet genotypes for resistance to hyperthermia and obtain temperatureresistant lines. Nine hybrids and one variety of sugar beet were screened. Cotyledonary leaves and hypocotyls isolated from aseptic seedlings were used for induction of callus and subsequent subcultivation. To create hyperthermic conditions, the callus lines were maintained in thermostats at temperatures of +27 °C, +41 °C, +45 °C, and +47 °C. The effect of high temperatures on the callus tissue was assessed by the specific callus diameter index. The free proline was detected with chromatography. As result of callus tissue exposed to temperatures of +41 °C, 45 °C and 47 °C, on the 9th day of cultivation at high temperatures, significant differences were observed in the size and colouration of the callus tissues. At a moderate temperature (+41 °C), the growth of the callus mass was somewhat higher compared to the control. At a high temperature (+45 °C), the intensity of the growth processes decreased and ceased at a temperature of +47 °C. After transfer and subsequent cultivation of callus tissues in regeneration medium MSR -Murashige and Skoog medium for regeneration, all genotypes demonstrated the formation of morphological structures that initiated the formation of regenerated plants. The number of regenerated plants largely fluctuated over temperatures and almost was not related to genotypes. Consequently, the temperature-resistant lines obtained as a result of extreme heat treatment, differ in terms of the specific diameter of callus.

show abstract

Section: Resultssupporting

confidence: 89%

Obtaining Temperature-Resistant Sugar Beet Lines (<i>Beta vulgaris</i> L.)

Klyachenko¹,

Prysiazhniuk²,

Bokiy³

et al. 2023

Ecol. Eng. Environ. Technol.

View full text Add to dashboard Cite

show abstract

“…The environment before and after chilling also is important. Chilling damage is affected by the temperature, light conditions, and water status of the plants before chilling (Lafuente et al, 1991;Pomeroy and Mudd, 1987;Rietze and Wiebe, 1989;Rikin et al, 1976;Saltveit, 1991;Wilson and Crawford, 1974) and the light conditions after chilling (Lasley et al, 1979;Rietze and Wiebe, 1989). Watermelon is susceptible to CI but is more resistant than cucumber (Wehner and Mirdad, 1994).…”

mentioning

confidence: 99%

Tolerance of Watermelon Seedlings to Low-temperature Chilling Injury

Kozik¹,

Wehner²

2014

horts

View full text Add to dashboard Cite

Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is one of the Cucurbitaceae species and subtropical crops that exhibit chilling injury (CI) when exposed to low temperatures. Watermelon seedlings were tested for chilling tolerance using methods modified from cucumber. Three experiments were conducted using different combinations of chilling durations of 6, 12, 24, or 36 hours and chilling temperatures of 2 or 4 °C. Watermelon seedlings were more resistant to low temperatures than cucumber seedlings, so it was necessary to use long chilling durations to induce significant foliar damage. A diverse set of 16 watermelon cultigens was tested: Allsweet, Black Diamond, Chubby Gray, Charlee, Charleston Gray, Dixielee, Golden, Golden Honey, New Winter, NH Midget, Sugar Baby, Sugarlee, Sunshade, PI 189225, PI 244018, and PI 595203. Experiments were conducted in a controlled environment with a light intensity of 500 mmol·m−2·s−1 photosynthetic photon flux density (PPFD). Optimal conditions for chilling treatment were 36 hours at 4 °C or 24 hours at 2 °C. The most resistant cultigen was PI 244018, and the most susceptible cultigens were NH Midget and Golden.

show abstract

“…Plants are generally more sensitive to low temperature in light than in dark (Pomeroy and Mudd 1987). The evidence is convincing that the chilling-induced photooxidation of leaf pigments (Halliwell 1987, Wise andNaylor 1987a) is linked directly to the leakage of photosynthetic electrons to molecular oxygen to form one or more potentially toxic oxygen species.…”

mentioning

confidence: 99%

Does the alternative pathway ameliorate chilling injury in sensitive plant tissues?

Purvis¹,

Shewfelt²

1993

Physiol Plant

125

161

View full text Add to dashboard Cite

Does the alternative pathway amehorate chilling injury in sensitive plant tissues? -Physiol. Plant. 88: 712-718.Free radical processes have been observed in senescence and several membraneassociated disorders of plants including chilling, freezing, and desiccation injuries. The mitochondria ot plant tissues exposed to low temperatures, and other abiotic and biotic stresses, produce superoxide and/or hydrogen peroxide when electron transport through the cytochrome pathway is impaired due to the energy state of the cell or to stress-induced physical changes in the membrane components. The superoxide and/or hydrogen peroxide produced can diffuse throughout the cell causing peroxidation of membrane lipids which results in membrane disruption, increased permeability and metabolic disturbances, and eventually the visible symptoms of chilling injury. The alternative pathway of electron transport in the mitochondria, which is induced by low temperatures in some plant tissues, can mediate these degradative processes by reducing the level of superoxide generated by the mitochondria.

show abstract

Chilling Sensitivity of Cucumber Cotyledon Protoplasts and Seedlings

Cited by 9 publications

References 14 publications

Obtaining Temperature-Resistant Sugar Beet Lines (<i>Beta vulgaris</i> L.)

Obtaining Temperature-Resistant Sugar Beet Lines (<i>Beta vulgaris</i> L.)

Tolerance of Watermelon Seedlings to Low-temperature Chilling Injury

Does the alternative pathway ameliorate chilling injury in sensitive plant tissues?

Contact Info

Product

Resources

About