Chilling vs. Other Methods of Immobilizing Flies2

Harris, Robert L.; Hoffman, R. A.; Frazar, E. D.

doi:10.1093/jee/58.2.379a

Cited by 18 publications

(12 citation statements)

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“…There is scant information on the use of N 2 and low temperature to anesthetize insects. Harris et al (1965) found that Stomoxys calcitrans (L.) and Haematobia irritans (L.) were affected more adversely by exposure to CO 2 than to low temperature. Hooper (1970) found that N 2 had a less adverse effect than CO 2 on survival, fecundity, and egg hatch in the Mediterranean fruit fly.…”

Section: Introductionmentioning

confidence: 97%

Comparative effect of short term exposures of Callosobruchus subinnotatus to carbon dioxide, nitrogen, or low temperature on behavior and fecundity

Mbata

Ramaswamy

Reichmuth

1998

Entomologia Exp Applicata

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The effect of anesthetizing with carbon dioxide, nitrogen, or low temperature on the reproductive behavior and fecundity of Callosobruchus subinnotatus was investigated. Males and females anesthetized with N2 as virgins had shorter recovery time, copulated earlier, and had shorter mating time than those subjected to CO2 or low temperature. Exposure of males and females to 2.0±0.5 °C for 10 min had the most profound effect on the recovery periods, copulation time, and duration of mating. Adults anesthetized with CO2 and low temperature laid fewer eggs than those anesthetized with N2. Females were more affected than males by the treatments, except when males were chilled for 10 min, and this prolonged the time for commencement of mating. The mode of anesthesia could affect egg production because vitellogenesis and choriogenesis take place in the imago stage of bruchids.

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Section: Introductionmentioning

confidence: 97%

Comparative effect of short term exposures of Callosobruchus subinnotatus to carbon dioxide, nitrogen, or low temperature on behavior and fecundity

Mbata

Ramaswamy

Reichmuth

1998

Entomologia Exp Applicata

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“…Intrathoracic injection is the most direct means of dsRNA delivery and has been used successfully in multiple insect species to induce RNAi (recently reviewed by Scott et al, 2013). Injection requires immobilization of insects by either cold treatment (Harris et al, 1965) or exposure to CO 2 (Blandin et al, 2002).…”

Section: Delivery Of Dsrna Into Adult C Sonorensismentioning

confidence: 99%

Inducing RNA interference in the arbovirus vector, Culicoides sonorensis

Mills

Nayduch

Michel

2014

Insect Molecular Biology

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Biting midges in the genus Culicoides are important vectors of arboviral diseases, including epizootic hemorrhagic disease, bluetongue, and likely Schmallenberg, which cause significant economic burden worldwide. Research on these vectors has been hindered by the lack of a sequenced genome, the difficulty of consistent culturing of certain species, and the absence of molecular techniques such as RNA interference (RNAi). Here, we report the establishment of RNAi as a research tool for the adult midge, Culicoides sonorensis. Based on previous research and transcriptome analysis, which revealed putative siRNA pathway member orthologs, we hypothesized that adult C. sonorensis midges have the molecular machinery needed to preform RNA silencing. Injection of control dsRNA, dsGFP, into the hemocoel 2–3 day old adult female midges resulted in survival curves that support virus transmission. DsRNA injection targeting the newly identified C. sonorensis inhibitor of apoptosis protein 1 (CsIAP1) ortholog, resulted in a 40% decrease of transcript levels and 73% shortened median survivals as compared to dsGFP-injected controls. These results reveal the conserved function of IAP1. Importantly, they also demonstrate the feasibility of RNAi by dsRNA injection in adult midges, which will greatly facilitate studies of the underlying mechanisms of vector competence in C. sonorensis.

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“…The flies are therefore anesthetised with carbon dioxide, a procedure which results in extension of the ovipositor in the female and allows a clear distinction from the male to be made. Since CO, administered over longer periods can be harmful (Harris et al 1965), the duration of anesthetisation is kept as short as possible.…”

Section: Adults the Cages Used (mentioning

confidence: 99%

AN IMPROVED MASS-REARING METHOD OF THE CARROT RUST FLY, PSILA ROSAE (DIPTERA: PSILIDAE)

Städler¹

1971

Can Entomol

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Can. Ent. 103: 1033Ent. 103: -1038Ent. 103: (1971 Protein fed to adults of the carrot rust fly, Psila rosae F., enhanced oviposition: the addition of yeast hydrolysate to the sugar diet resulted in a 50% increase in oviposition, giving an average of 75 eggs per female.A description is given of a simple oviposition device, which allows normal oviposition (compared with sand).Carrots planted in sand gave best rearing results when inoculated with eggs 14 days after insertion. Germinating carrot seeds placed on top of the sand improved the rate of development of the eggs to pupae from 40% to 62%.

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Chilling vs. Other Methods of Immobilizing Flies2

Cited by 18 publications

References 0 publications

Comparative effect of short term exposures of Callosobruchus subinnotatus to carbon dioxide, nitrogen, or low temperature on behavior and fecundity

Comparative effect of short term exposures of Callosobruchus subinnotatus to carbon dioxide, nitrogen, or low temperature on behavior and fecundity

Inducing RNA interference in the arbovirus vector, Culicoides sonorensis

AN IMPROVED MASS-REARING METHOD OF THE CARROT RUST FLY, PSILA ROSAE (DIPTERA: PSILIDAE)

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