Comparative effect of short term exposures of Callosobruchus subinnotatus to carbon dioxide, nitrogen, or low temperature on behavior and fecundity

Abstract

The effect of anesthetizing with carbon dioxide, nitrogen, or low temperature on the reproductive behavior and fecundity of Callosobruchus subinnotatus was investigated. Males and females anesthetized with N2 as virgins had shorter recovery time, copulated earlier, and had shorter mating time than those subjected to CO2 or low temperature. Exposure of males and females to 2.0±0.5 °C for 10 min had the most profound effect on the recovery periods, copulation time, and duration of mating. Adults anesthetized with CO2 and low temperature laid fewer eggs than those anesthetized with N2. Females were more affected than males by the treatments, except when males were chilled for 10 min, and this prolonged the time for commencement of mating. The mode of anesthesia could affect egg production because vitellogenesis and choriogenesis take place in the imago stage of bruchids.

Flea fitness is reduced by high fractional concentrations of CO2 that simulate levels found in their hosts' burrows

“…Our results partially concur with those from experiments with D. melanogaster where it was found that flies laid fewer eggs and the fraction of eggs that hatched decreased when in a F CO2 of 0.13; the decrease was even greater when F CO2 was 0.20 (Helenius et al, 2009). In addition, exposure of insects to sublethal doses of CO 2 (often F CO2 =1) for short durations for anesthetic purposes resulted in insects laying fewer eggs and in reduced hatching success of those eggs (Press et al, 1973;Mbata et al, 1998). Differences regarding the viability of eggs between the present study and other studies might result from differences in experimental F CO2 and duration of exposure.…”

Efficacy of Controlled Atmosphere Treatments to Manage Arthropod Pests of Dry-Cured Hams

“…Depending on the temperature, CO 2 treatments may take from a few days to several weeks to be effective in gas-tight chambers or silos [ 15 ]. CA has distinct biological effects on insects, and these effects can be both physiological [ 15 , 16 ] and behavioral [ 17 ]. A CA of low oxygen can be achieved either by purging the storage containers with an inert gas, such as nitrogen, which forces most other gases out and, thus, decreases O 2 concentration, or it can be achieved by imposing a low pressure via vacuum, which reduces concentrations of all gases in the system [ 18 ].…”