Chimaerism and erythroid marker expression after microinjection of human acute myeloid leukaemia cells into murine blastocysts

Generation of human/rat xenograft animal model for the study of human donor stem cell behaviorsin vivo

“…blastocyst and in uter o ) may be provided with all the possible lineage options available during development [16] . But as shown in Figure 2, higher levels of human cell engraftment occurred in solid organs examined, compared with blood, which was consistent with the goat results reported by Zeng et al [8] ; in addition, the chimerism was also nonuniformly distributed over all solid organs analyzed, as mentioned in mice [5,17,18] . The different patterns of human cell distribution over all tissues analyzed in individual animal may be largely owing to the xenogeneic setting diversity of recipients, cell sources, the discrepancy of cellular components of transplanted cells from various donors, the transplantation routes (such as blastocyst injection and in utero transplantation), non-consistency (such as site and time of injection) of manipulation each time and other unknown reasons.…”

“…Optimal transplantation approach and time When hUCB-derived CD34 + cells [18] , human acute myeloid leukemia cells [17] and mouse FDCPmix cells (murine progenitor cells of haematopoietic origin; Petrovic et al [21] ) were microinjected into murine blastocysts, respectively, they preferentially engrafted in PB and yolk sac of embryos, and less frequently detected in other tissues of embryos or adults.…”

The Potential of Rat Inner Cell Mass and Fetal Neural Stem Cells to Generate Chimeras

“…Spontaneous cell fusion has been widely observed in NS cells and bone marrow cells when they were cultured in vitro or following transplantation in vivo (Wang et al, 2003;Willenbring et al, 2004). Thus, chimeric fetuses and adult tissues produced by blastocyst injection with hematopoietic stem cells, acute myeloid leukaemia cells, and neural stem cells could be caused by cell fusion between donor and recipient cells (Geiger et al, 1998;Durr et al, 2003). Our observation of positive staining for donor FNS cells was detected in chimeric fetuses but not in the tissues of newborn pups.…”

“…They also can be reprogrammed to develop into cloned offspring by nuclear transfer (Kawase et al, 2000). Mouse blastocyst is the good microenvironment not only for mouse ES cells or murine hematopoietic stem cells to chimerically redevelop into fetal and adult tissues (Geiger et al, 1998) but also for human hematopoietic stem cells (Harder et al, 2002) and human acute myeloid leukaemia cells (Harder et al, 2003) to redevelop. In the report, murine NS cells contributed to chimeric fetal liver, yolk sac and peripheral blood, but chimeric cells only were detected in adult neural tissues (Harder et al, 2004).…”

Differentiation Potential of FDCPmix Cells following Injection into Blastocysts

“…In this way, it has been demonstrated that the microenvironment of the preimplantation embryo not only supports the development of murine haematopoietic (HSCs) and neural stem cells (NSCs) but also of human cord-blood-derived HSCs Harder et al, 2002b;Jiang et al, 2002;Kirchhof et al, 2002;Dürr et al, 2003]. In addition, microinjection of human KG-1 myeloid leukaemia cells and primary human acute myeloid leukaemia (AML) cells led to the generation of chimaeric embryos and adults, and to the generation of differentiated progeny originating from the injected leukaemia cells [Dürr et al, 2003]. Surprisingly, chimaeric embryos and adults showed no signs of tumour formation.…”