Differentiation Potential of FDCPmix Cells following Injection into Blastocysts

Abstract

Factor-dependent cell Paterson mixed potential (FDCPmix) cells are murine, multipotent, interleukin-3 (IL-3)-dependent progenitor cells, established from long-term bone marrow cultures. They show multilineage myeloid/erythroid and osteoclast differentiation capacity in vitro. FDCPmix cells are neither immortalised, leukaemic nor transformed and have no detectable karyotypic abnormalities. To investigate the broader differentiation potential of FDCPmix cells in vivo, they were injected into murine blastocysts, and the engraftment of donor cells in developing chimaeric embryos was analysed. FDCPmix cell progeny was detected predominantly in haematopoietic tissues, and immunohistochemical analysis revealed that the majority of donor-derived cells expressed the pan-haematopoietic marker CD45. Albumin expression indicative of hepatocytes was not detectable in FDCPmix-derived cells in chimaeric foetal livers. However, analysis of foetal brains of developing embryos revealed a low frequency of neural cell adhesion molecule-positive donor cells. These results suggest that the majority of FDCPmix cells injected into blastocysts follow haematopoietic differentiation programs, but that a small number of cells can assume the expression of neural markers.

Generation of human/rat xenograft animal model for the study of human donor stem cell behaviorsin vivo

“…Optimal transplantation approach and time When hUCB-derived CD34 + cells [18] , human acute myeloid leukemia cells [17] and mouse FDCPmix cells (murine progenitor cells of haematopoietic origin; Petrovic et al [21] ) were microinjected into murine blastocysts, respectively, they preferentially engrafted in PB and yolk sac of embryos, and less frequently detected in other tissues of embryos or adults.…”

Acute Hepatic Failure-Derived Bone Marrow Mesenchymal Stem Cells Express Hepatic Progenitor Cell Genes

“…Since the potential of BMSCs to differentiate into hepatocytes was first demonstrated [Petersen et al, 1999], the capacity of BMSCs to differentiate into liver cells has been validated repeatedly [Avital et al, 2001[Avital et al, , 2002Petrovic et al, 2004;Grompe, 2005]. Furthermore, several recent reports have shown that HPCs can be derived from BMSCs [Theise et al, 2000;Thorgeirsson and Grisham, 2006].…”

Potentiality of embryonic stem cells: an ethical problem even with alternative stem cell sources

“…No report exists in the literature about successful tetraploid complementation with somatic stem cells. Somatic stem cells can form chimeras when combined with normal (but not tetraploid) embryos and they then show their plasticity by contributing to the formation of various organs and tissues (the high plasticity reported in Clarke et al 39 has been debated by other groups 40 and seems to depend considerably on the cell type). The formation of a whole embryo or living animal from somatic stem cells by tetraploid complementation has not been reported in the literature and does not seem to be possible.…”