Chinese Herbal Medicine and Its Regulatory Effects on Tumor Related T Cells

Hoffman, Robert D.; Li, Changyu; He, Kai Li; Wu, Xiaoxing; He, Tong‐Chuan; Gao, Jian‐Li

doi:10.3389/fphar.2020.00492

Cited by 27 publications

(19 citation statements)

References 113 publications

(123 reference statements)

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“…Then the culture medium was discarded. After that, the cells were cultured with the culture medium containing 0, 0.4, 0.8 and 1.2 mg/mL (11) of S. lyratum polysaccharide, which was marked as the control group, S. lyratum polysaccharide L group, S. lyratum polysaccharide M group, S. lyratum polysaccharide H group. After 24 h of incubation, 10μL CCK-8 was added and incubated for 2 h, and the optical density (OD) was measured by a microplate reader (wavelength 450 nm).…”

Section: Detection Of the Inhibition Rate Of Cell Proliferation By Cc...mentioning

confidence: 99%

Effect of Solanum lyratum Polysaccharide on Malignant Behaviors of Lung Cancer Cells by Regulating the Circ_UHRF1/miR-513b-5p Axis

Feng

Wang

Liu

2022

Cell Mol Biol (Noisy-le-grand)

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This study aimed to investigate the effect of Solanum lyratum polysaccharide on the malignant behavior of lung cancer cells and its possible mechanism. For this purpose, lung cancer A549 cells were cultured in vitro and treated with different doses (0.4, 0.8, 1.2 mg/mL) of Solanum lyratum polysaccharide for 24 h. Then cell proliferation was detected by the CCK-8 method and clone formation test. Transwell test was used to detect cell migration and invasion, and flow cytometry was used to detect cell apoptosis. The protein expressions of Bax and Bcl-2 in cells were detected by Western blotting, and the protein expressions of circ_UHRF1 and miR-513b-5p were detected by the qRT-PCR method. Pearson correlation was used to analyze the correlation between circ_UHRF1 and miR-513b-5p expressions in lung cancer tissues. Results showed that compared with the control group, the proliferation inhibition rate and apoptosis rate of A549 cells that intervened with the Solanum lyratum polysaccharide and expression of Bax protein in the cells were all increased (P<0.05), but the number of clones, migration and invasion and the protein expression of Bcl-2 were all decreased (P<0.05), and were dose-dependent. The expression of circ_UHRF1 in A549 cells that intervened with the S. lyratum polysaccharide was decreased (P<0.05), but the expression of miR-513b-5p was increased (P<0.05). The expression of circ_UHRF1 in lung cancer tissues was higher than that of adjacent tissues (P<0.05), and the expression of miR-513b-5p was lower than that of adjacent tissues (P<0.05). The expressions of circ_UHRF1 and miR-513b-5p in lung cancer tissues were negatively correlated (r=-0.861, P<0.05). Circ_UHRF1 could target miR-513b-5p, and the expression of miR-513b-5p in A549 cells knocking down circ_UHRF1 was increased. After knocking down circ_UHRF1, the proliferation inhibition rate and apoptosis rate of A549 cells and protein expression of Bax in the cells were all increased (P<0.05), but the number of clones, migration and invasion and the protein expression of Bcl-2 were all decreased (P<0.05). Up-regulation of circ_UHRF1 reduced the effects of S. lyratum polysaccharide on the proliferation, migration, invasion and apoptosis of A549 cells. In general, S. lyratum polysaccharide could inhibit the proliferation, migration and invasion of lung cancer A549 cells, and induce cell apoptosis. Its mechanism may be related to the regulation of the circ_UHRF1/miR-513b-5p axis.

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Section: Detection Of the Inhibition Rate Of Cell Proliferation By Cc...mentioning

confidence: 99%

Effect of Solanum lyratum Polysaccharide on Malignant Behaviors of Lung Cancer Cells by Regulating the Circ_UHRF1/miR-513b-5p Axis

Feng

Wang

Liu

2022

Cell Mol Biol (Noisy-le-grand)

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“…On the other hand, deficient immune response results in chronic and persistent bacterial infections (Smith et al, 2009). Immunomodulation is an important function of TCM, which activates or suppresses the immune responses of a variety of immune cells, including macrophages (Jian et al, 2019), dendritic cells (Chen et al, 2006), T cells (Guo et al, 2015), B cells (Kawakita et al, 1987a) and NK cells (Hoffman et al, 2020). The immunomodulatory effects of TCM on host immunity in the context of P. aeruginosa infections have been evaluated in past decade (Table 5) (Kong et al, 2015;Hou et al, 2016;.…”

Section: Immunomodulationmentioning

confidence: 99%

Traditional Chinese Medicine is an Alternative Therapeutic Option for Treatment of Pseudomonas aeruginosa Infections

Pang

Zhu

2021

Front. Pharmacol.

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Pseudomonas aeruginosa is an opportunistic pathogen causing life-threatening infections in cystic fibrosis patients and immunocompromised individuals, and it is a leading cause of nosocomial infections associated with significant morbidity and mortality. Treatment of P. aeruginosa infections is challenging due to the antibiotic resistance to most of the conventional antibiotics. Development of alternative therapeutic options is urgently demanded for the patients who have antibiotic-resistant infections. Traditional Chinese medicine (TCM) has a clinical history of thousands of years for prevention and treatment of infectious diseases in China, taking advantages of improving clinical outcomes, producing less side effects, inhibiting pathogen, and modulating host immunity. Recent research has revealed a variety of natural products derived from TCM showing significant antimicrobial effects on antibiotic-resistant strains of P. aeruginosa alone or combined with antibiotics in vitro or in animal models, suggesting that TCM is a promising complementary and alternative therapeutic approach for treatment of chronic P. aeruginosa infections. This review summarizes the recent findings attempting to dissect the mechanisms of TCM combating P. aeruginosa infections and highlights the molecular targets of TCM on P. aeruginosa and host.

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“…In the past decades, Chinese medicine therapy has shown remarkable potential as an alternative for cervical cancer treatment or as auxiliary reagents that support cancer treatment owing to their high efficiency and low toxicity [ 4 ]. An increasing number of natural antineoplastic agents were employed in the preclinical and clinical assessments.…”

Section: Introductionmentioning

confidence: 99%

Osthole Induces Apoptosis and Inhibits Proliferation, Invasion, and Migration of Human Cervical Carcinoma HeLa Cells

Liu

Wang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Purpose. To study the effect of osthole extract on proliferation, migration, invasion, and apoptosis of human cervical carcinoma HeLa cells and investigate its underlying mechanism. Methods. HeLa cells were exposed to osthole at various concentrations. Cell viability, migration, and invasion were detected by MTT assay, scratch wound-healing assay, and invasion assay, respectively. The proportion of cells undergoing apoptosis was analyzed by flow cytometry. Western blot and RT-qPCR were performed to determine changes in the expression of key factors in the Wnt/β-catenin signaling pathway. Results. The osthole extract effectively inhibited the proliferation, migration, and invasion potential of HeLa cells in a dose-dependent manner. The rate of apoptosis induction in HeLa cells treated with the osthole extract for 48 h was significantly higher than that of the untreated controls. Outcomes of the western blotting analysis and RT-qPCR showed that the expression of β-catenin, c-Myc, cyclin D1, survivin, and MMP-9 was significantly inhibited. Conclusion. Osthole could significantly inhibit the malignant behavior of HeLa cells and induce cellular apoptosis. Inactivation of the Wnt/β-catenin signaling pathway by osthole may be a mechanism to control cancer metastasis.

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Chinese Herbal Medicine and Its Regulatory Effects on Tumor Related T Cells

Cited by 27 publications

References 113 publications

Effect of Solanum lyratum Polysaccharide on Malignant Behaviors of Lung Cancer Cells by Regulating the Circ_UHRF1/miR-513b-5p Axis

Effect of Solanum lyratum Polysaccharide on Malignant Behaviors of Lung Cancer Cells by Regulating the Circ_UHRF1/miR-513b-5p Axis

Traditional Chinese Medicine is an Alternative Therapeutic Option for Treatment of Pseudomonas aeruginosa Infections

Osthole Induces Apoptosis and Inhibits Proliferation, Invasion, and Migration of Human Cervical Carcinoma HeLa Cells

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