Chinese population data on three tetrameric short tandem repeat loci — HUMTHO1, TPOX, and CSF1PO — derived using multiplex PCR and manual typing

Huang, Nu En; Schumm, James W.; Budowle, Bruce

doi:10.1016/0379-0738(94)01646-m

Cited by 61 publications

(30 citation statements)

References 8 publications

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“…HUMTPOX is more disciminating in our Japanese sample than in other population samples, e.g. Chinese (Hexp: 0.59, MEC: 0.31, PD: 0.75) (Huang et al 1995), German Caucasians (H-exp: 0.59, MEC: 0.34, PD: 0.77) (Puers et al 1993b), and Swiss Caucasians (H-exp: 0.59, MEC: 0.35, PD: 0.78) (Hochmeister et al 1995). This result suggests that HUMTPOX is a more appropriate locus for forensic use in the Japanese population than in the other populations compared.…”

Section: Resultsmentioning

confidence: 90%

Analysis of the STR loci HUMF13A01, HUMFXIIIB, HUMLIPOL, HUMTH01, HUMTPOX and HUMVWFA31 in a Japanese population

et al. 1996

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Population studies on six short tandem repeat loci, HUMF13A01, HUMFXIIIB, HUMLIPOL, HUMTH01, HUMTPOX and HUMVWFA31 were carried out in a sample of unrelated Japanese individuals (n = 337-545) living in Gifu Prefecture (central region of Japan). Five alleles could be identified for HUMFXIIIB, six for HUMF13A01, HUMLIPOL, HUMTH01 and HUMTPOX, and eight for HUMVWFA31. For all/six loci no deviations from the Hardy-Weinberg equilibrium hypothesis were detected. The mean exclusion chance ranged from 0.22 to 0.60, the power of discrimination from 0.63 to 0.93, and the expected heterozygosity from 0.43 to 0.80. Allele frequency distributions for the loci in the Japanese sample were not similar to those in samples from other racial or ethnic groups except for the Chinese (for HUMTPOX). The results demonstrate that HUMTH01, HUMTPOX and HUMVWFA31 are more useful for forensic investigations in the Japanese population that the other three loci.

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Section: Resultsmentioning

confidence: 90%

Analysis of the STR loci HUMF13A01, HUMFXIIIB, HUMLIPOL, HUMTH01, HUMTPOX and HUMVWFA31 in a Japanese population

et al. 1996

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“…The multiplex analysis of HUMTH01, TPOX, and CSF1PO was performed as described by Huang et al [12] and the manufacturer's recommendations [15] The frequency of each allele for each STR was calculated from the 7 numbers of each genotype in the sample set (i.e. the gene count method).…”

Section: Multiplex Pcr Analysismentioning

confidence: 99%

“…Since the sizes of the 3 STR loci in the multiplex analysis do not overlap, unequivocal typing of the 3 loci is possible. Currently there are little data on allele frequencies and genotype distributions in various populations for the STR loci HUMTH01, TPOX, and CSF1PO [12]. This paper presents allele/genotype frequency data in a Swiss population sample for these 3 tetrameric STR loci generated using a STR Multiplex System.…”

Section: Introductionmentioning

confidence: 97%

Swiss population data and forensic efficiency values on 3 tetrameric short tandem repeat loci-HUMTH01, TPOX, and CSFIPO - derived using a STR multiplex system

Hochmeister¹,

Budowle

Schumm

et al. 1995

Int J Leg Med

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Allele and genotype frequencies for 3 tetrameric short tandem repeat loci were determined in a Swiss population sample (n = 100) using the GenePrint STR Multiplex System, electrophoresis of the PCR products in DNA sequencing gels and subsequent detection of allelic fragments by silver staining. The loci are HUMTH01, TPOX, and CSF1PO. The observed heterozygosities are 83.0%, 60.0%, and 72.0%, respectively. The discrimination power determined for the individual loci is 0.914, 0.780, and 0.860, respectively, and the combined discrimination power for the triplex is 0.997. All loci meet Hardy-Weinberg expectations and after Bonferroni correction there was no evidence that the population sample deviates from expectations of independence. Moreover, independence of alleles at these STR loci with other PCR-based loci derived from the same Swiss population sample, previously reported, were considered. These loci were DQA1, LDLR, GYPA, HBGG, D7S8, GC and D1S80. Again, after Bonferroni correction there was no evidence that the population sample deviates from expectations of independence among alleles at the 10 different PCR-based loci. Thus, the allelic frequency data can be used in human identity testing to estimate the frequency of a multiple PCR-based DNA profile in the Swiss population.

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“…Note that the combined power of discrimination exceeded 0.9998. In addition, we compared all 195 profiles in the database pairwise (18,195 pairs of profiles), finding one pair of individuals that matched at all 4 loci, and 82 pairs that matched at 3 loci.…”

Section: Sample Statisticsmentioning

confidence: 99%

A Dutch population study of the STR loci HUMTHO1, HUMFES/FPS, HUMVWA31/1 and HUMF13A1, conducted for forensic purposes

Sjerps

Pieron

et al. 1995

Int J Leg Med

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We report on a Dutch population study of the STR loci HUMTHO1, HUMFES/FPS, HUMVWA31/1, and HUMF13A1, in which we used multiplex amplification and automated fragment detection. Genotype and allele frequencies showed no deviation from Hardy-Weinberg and linkage equilibrium. The improved Bonferroni procedure was used to combine the results of several tests. The power of discrimination of a complete profile exceeded 0.9998. We compared the allele frequencies in the Dutch sample to the frequencies in other populations using a bipilot to visualize alleles and populations simultaneously. The Dutch sample was similar to most other Caucasian samples. The data demonstrate that the genetic systems in this report are a valuable tool for forensic identity testing in The Netherlands.

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Chinese population data on three tetrameric short tandem repeat loci — HUMTHO1, TPOX, and CSF1PO — derived using multiplex PCR and manual typing

Cited by 61 publications

References 8 publications

Analysis of the STR loci HUMF13A01, HUMFXIIIB, HUMLIPOL, HUMTH01, HUMTPOX and HUMVWFA31 in a Japanese population

Analysis of the STR loci HUMF13A01, HUMFXIIIB, HUMLIPOL, HUMTH01, HUMTPOX and HUMVWFA31 in a Japanese population

Swiss population data and forensic efficiency values on 3 tetrameric short tandem repeat loci-HUMTH01, TPOX, and CSFIPO - derived using a STR multiplex system

A Dutch population study of the STR loci HUMTHO1, HUMFES/FPS, HUMVWA31/1 and HUMF13A1, conducted for forensic purposes

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