Chip-MS: A Polymeric Microfluidic Device with Integrated Mass-Spectrometer Interface

Killeen, Kevin; Yin, Hongfeng; Udiavar, S.; Brennen, Reid A.; Juanitas, Mark; Poon, E.; Sobek, Dan; Goor, Tom van de

doi:10.1007/978-94-010-1015-3_141

Cited by 12 publications

(11 citation statements)

References 3 publications

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“…This approach seems like a very reasonable and lowcost solution to the problem of controlling the fluid in the high-voltage field at the chip exit of ESI. An alternative ESI interface, equally interesting from a scientific as well as commercially point of view was demonstrated by Killeen et al (Agilent Technologies, Paolo Alto, CA, USA), with a complete system fabricated in laminated polyimide sheets that hold both a micro-LC column and upstream interface to conventional rotary valves for sample injection and eluent control [52]. The chip outlet is defined as a fine laser cut tip at the end of the separation channel, providing a robust and simple ESI interface.…”

Section: Microchip Technologies For Electrospray Msmentioning

confidence: 97%

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New directions of miniaturization within the biomarker research area

2004

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An overview of the current trends within protein expression profiling is given where multidimensional separation of both gel and liquid phase techniques linked to mass spectrometry is viewed as a major route in the global proteome mapping. A clear trend in these biochemical developments is the effort to sequence and identify low-abundant protein expressions where assay miniaturization and integrated sample processing play a central role. Two areas of miniaturization within the proteomics field are addressed: (i) sample cleanup and enrichment, and (ii) silicon microstructure developments for protein chip microarrays.

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Section: Microchip Technologies For Electrospray Msmentioning

confidence: 97%

“…The direction that the developments are taking is to develop robust and high-throughput solutions to the users. In this context, interfacing chip-integrated protocols to ESI-MS has been a long sought goal and several promising approaches have emerged lately [47][48][49][50][51][52].…”

Section: Microchip Technologies For Electrospray Msmentioning

confidence: 99%

New directions of miniaturization within the biomarker research area

2004

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“…There is a commercial microfluidic ESI device now on the market [66]. The channel was built by laser ablation on the PI film and a platinum electrode was deposited at the end of the channel to apply the voltage for ESI.…”

Section: Functional Integration Towards Micrototal Analysis For Protementioning

confidence: 99%

Chip-based microfluidic devices coupled with electrospray ionization-mass spectrometry

2005

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We present the current status of the development of microfluidic devices fabricated on different substrates for coupling with electrospray ionization-mass spectrometry (ESI-MS). Until now, much success has been gained in fabricating the ESI chips, which show better performances due to miniaturization when compared with traditional methods. Integration of multiple steps for sample preparation and ESI sample introduction, however, remains a great challenge. This review covers the main technical development of electrospray device that were published from 1997 to 2004. This article does not attempt to be exclusive. Instead, it focuses on the publications that illustrated the breath of the development and applications of microchip devices for MS-based analysis.

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“…The fabrication process was relatively difficult and time-consuming but the chips had four walls of parylene and the accurately defined nozzles resemble traditional nanospray tips. Polyimides have also been used as structural material for ESI tips [19][20][21]. Plasma etching of polyimide films has been used to produce ESI source with integrated desalting membrane [19,20] and LC integrated with ESI has been made by laser ablation [21].…”

Section: Introductionmentioning

confidence: 99%

“…Polymeric ESI systems have been made also by lithography, laser ablation, and etching of patterns directly to polymer [17][18][19][20][21]. Normally these direct fabrication methods enable fabrication of more advanced structures, but the fabrication processes become more difficult.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of enclosed SU-8 tips for electrospray ionization-mass spectrometry

Tuomikoski¹,

Sikanen

Ketola

et al. 2005

Electrophoresis

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We describe a novel electrospray tip design for MS which is fabricated completely out of SU-8 photoepoxy. A three-layer SU-8 fabrication process provides fully enclosed channels and tips. The tip shape and alignment of all SU-8 layers is done lithographically and is therefore very accurate. Fabrication process enables easy integration of additional fluidic functions on the same chip. Separation channels can be made with exactly the same process. Fluidic inlets are made in SU-8 during the fabrication process and no drilling or other postprocessing is needed. Channels have been fabricated and tested in the size range of 10 microm x 10 microm-50 microm x 200 microm. Mass spectrometric performance of the tips has been demonstrated with both pressure-driven flow and EOF. SU-8 microtips have been shown to produce stable electrospray with EOF in a timescale of tens of minutes. With pressure driven flow stable spray is maintained for hours. Taylor cone was shown to be small in volume and well defined even with the largest channel cross section. The spray was also shown to be well directed with our tip design.

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Chip-MS: A Polymeric Microfluidic Device with Integrated Mass-Spectrometer Interface

Cited by 12 publications

References 3 publications

New directions of miniaturization within the biomarker research area

New directions of miniaturization within the biomarker research area

Chip-based microfluidic devices coupled with electrospray ionization-mass spectrometry

Fabrication of enclosed SU-8 tips for electrospray ionization-mass spectrometry

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