Chip-based microfluidic devices coupled with electrospray ionization-mass spectrometry

Abstract

We present the current status of the development of microfluidic devices fabricated on different substrates for coupling with electrospray ionization-mass spectrometry (ESI-MS). Until now, much success has been gained in fabricating the ESI chips, which show better performances due to miniaturization when compared with traditional methods. Integration of multiple steps for sample preparation and ESI sample introduction, however, remains a great challenge. This review covers the main technical development of electrospray device that were published from 1997 to 2004. This article does not attempt to be exclusive. Instead, it focuses on the publications that illustrated the breath of the development and applications of microchip devices for MS-based analysis.

Recent advances in the application of capillary electromigration methods for food analysis

“…Thus, as can be deduced from Table 4, microchips have already been applied in food analysis to differentiate species [175], to detect food spoilage bacteria [98], to detect GMOs [176,184], and to analyze small organic and inorganic compounds such as amino acids, sugars, etc [177][178][179][180][181][182][183]. Moreover, different detection schemes have been developed to be used together with microchips including LIF [98,175,176,184], conductivity [178][179][180][181], amperometric [182,183] and MS detectors [186]. This will make possible to address and solve an even wider number of problems in food science.…”

Microssistemas de análises químicas: introdução, tecnologias de fabricação, instrumentação e aplicações

“…Essa tradicional metodologia, embora bastante útil e poderosa, exige muito tempo e trabalho laboratorial. Nesse contexto, os microdispositivos oferecem novas oportunidades que não existem nas técnicas tradicionais de análise proteômica: rapidez, análise simultânea de amostras e potencial para controle e automação das várias etapas da análise 117 . A maior parte dos desenvolvimentos recentes em microfluídica aplicada à análise proteômica está voltada para a combinação, em um único microdispositivo, da separação por eletroforese uni ou multi-dimensional e detecção por MS. Uma vez que a espectrometria de massas é a técnica mais usada para análise e caracterização de proteínas, vários trabalhos voltados ao acoplamento de microdispositivos com MS têm sido relatados 26 .…”

Three-Dimensional Electro-Sonic Flow Focusing Ionization Microfluidic Chip for Mass Spectrometry

“…Given the rapid development of miniaturization technology, microfluidic chips have an important function in metabolomics, proteomics, and other biochemical analyses owing to their efficient and fast separations [4,5] in integrating complex sample pretreatment functions and their automatic manipulation of small sample volumes [6][7][8][9][10]. Therefore, the coupling of microfluidic chips with MS has received considerable research interest [11], particularly the design and improvement of chip-based microfluidic ionization sources coupled with MS, which has been comprehensively reviewed by a large number of research groups [12][13][14][15].…”