Chiral imaging of collagen by second-harmonic generation circular dichroism

Lee, Hsuan; Huttunen, Mikko J.; Hsu, Kuan-Lun; Partanen, Mari; Zhuo, Guan‐Yu; Kauranen, Martti; Chu, Shi‐Wei

doi:10.1364/boe.4.000909

Cited by 89 publications

(99 citation statements)

References 42 publications

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“…When susceptibilities are complex and the measurements are performed using linear polarizations, the real and imaginary parts of the susceptibility tensor are not separable, and the measured susceptibilities scale with the cosine of the phase difference between the susceptibility components . Previously modeled

χ_{xyz}^{(2)}

values from SHG‐CD measurements used different assumptions, which gave slightly larger values than our measurements . For extraction of complex susceptibility values, double Stokes‐Mueller polarimetry measurements can be employed, where the SHG Stokes vector is measured for a set of linear, circular and elliptical incoming polarization states .…”

Section: Resultsmentioning

confidence: 99%

“…In this paper, we extend our previous work to a more general symmetry class C 6 and extract the chiral component ratio of collagen,

C = χ_{xyz}^{{((), 2)}^{'}} / χ_{zxx}^{{((), 2)}^{'}}

. Chiroptical properties of collagen have been previously studied using circular polarizations via SHG circular dichroism (SHG‐CD) in thin films and tissues . In this study, we use linear polarizations to deduce the chiral ratio,

C = χ_{xyz}^{{((), 2)}^{'}} / χ_{zxx}^{{((), 2)}^{'}}

, of collagen in a tissue sample.…”

Section: Introductionmentioning

confidence: 94%

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Collagen chirality and three‐dimensional orientation studied with polarimetric second‐harmonic generation microscopy

Golaraei

Mirsanaye

et al. 2018

Journal of Biophotonics

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Polarization‐dependent second‐harmonic generation (P‐SHG) microscopy is used to characterize molecular nonlinear optical properties of collagen and determine a three‐dimensional (3D) orientation map of collagen fibers within a pig tendon. C6 symmetry is used to determine the nonlinear susceptibility tensor components ratios in the molecular frame of reference χzzz2/χzxx2 and χxyz2/χzxx2, where the latter is a newly extracted parameter from the P‐SHG images and is related to the chiral structure of collagen. The χxyz2/χzxx2 is observed for collagen fibers tilted out of the image plane, and can have positive or negative values, revealing the relative polarity of collagen fibers within the tissue. The P‐SHG imaging was performed using a linear polarization‐in polarization‐out (PIPO) method on thin sections of pig tendon cut at different angles. The nonlinear chiral properties of collagen can be used to construct the 3D organization of collagen in the tissue and determine the orientation‐independent molecular susceptibility ratios of collagen fibers in the molecular frame of reference.

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χ_{xyz}^{(2)}

Section: Resultsmentioning

confidence: 99%

“…In this paper, we extend our previous work to a more general symmetry class C 6 and extract the chiral component ratio of collagen,

C = χ_{xyz}^{{((), 2)}^{'}} / χ_{zxx}^{{((), 2)}^{'}}

C = χ_{xyz}^{{((), 2)}^{'}} / χ_{zxx}^{{((), 2)}^{'}}

, of collagen in a tissue sample.…”

Section: Introductionmentioning

confidence: 94%

Collagen chirality and three‐dimensional orientation studied with polarimetric second‐harmonic generation microscopy

Golaraei

Mirsanaye

et al. 2018

Journal of Biophotonics

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“…On the other hand, SHG is a popular technology that has gained a lot of attention more recently, and it is regularly being used for other softer tissues such as tendon [108], cartilage [112] or intervertebral disc [110]. However, similarly to PLM, SHG cannot be employed to quantitatively determine the collagen density and, hence, to derive the DO of the fibrils, but provides the in-plane [115] and/or out-of-plane [116] orientation in a semi-quantitative way [117].…”

Section: Polarized Second Harmonic Generation Imagingmentioning

confidence: 99%

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Georgiadis

Müller

Schneider

2016

J. R. Soc. Interface.

113

100

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Bone's remarkable mechanical properties are a result of its hierarchical structure. The mineralized collagen fibrils, made up of collagen fibrils and crystal platelets, are bone's building blocks at an ultrastructural level. The organization of bone's ultrastructure with respect to the orientation and arrangement of mineralized collagen fibrils has been the matter of numerous studies based on a variety of imaging techniques in the past decades. These techniques either exploit physical principles, such as polarization, diffraction or scattering to examine bone ultrastructure orientation and arrangement, or directly image the fibrils at the sub-micrometre scale. They make use of diverse probes such as visible light, X-rays and electrons at different scales, from centimetres down to nanometres. They allow imaging of bone sections or surfaces in two dimensions or investigating bone tissue truly in three dimensions, in vivo or ex vivo, and sometimes in combination with in situ mechanical experiments. The purpose of this review is to summarize and discuss this broad range of imaging techniques and the different modalities of their use, in order to discuss their advantages and limitations for the assessment of bone ultrastructure organization with respect to the orientation and arrangement of mineralized collagen fibrils.

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“…27 This work demonstrated for the first time the viability of the method in examining tissue structures with SHG-CD contrast, and this has now been reported for other tissues. 28 …”

Section: Introductionmentioning

confidence: 99%

Wavelength-Dependent Second Harmonic Generation Circular Dichroism for Differentiation of Col I and Col III Isoforms in Stromal Models of Ovarian Cancer Based on Intrinsic Chirality Differences

Campbell

Campagnola

2017

J. Phys. Chem. B

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Extensive remodeling of the extracellular matrix (ECM) occurs in many epithelial cancers. For example, in ovarian cancer, upregulation of collagen isoform type III has been linked to invasive forms of the disease, and this change may be a potential biomarker. To examine this possibility, we implemented wavelength-dependent second harmonic generation circular dichroism (SHG-CD) imaging microscopy to quantitatively determine changes in chirality in ECM models comprised of different Col I/Col III composition. In these models, Col III was varied between 0 and 40%, and we found increasing Col III results in reduced net chirality, consistent with structural biology studies of Col I and III in tissues where the isoforms comingle in the same fibrils. We further examined the wavelength dependence of the SHG-CD to both optimize the response and gain insight into the underlying mechanism. We found using shorter SHG excitation wavelengths resulted in increased SHG-CD sensitivity, where this is consistent with the electric-dipole-coupled oscillator model suggested previously for the nonlinear chirality response from thin films. Moreover, the sensitivity is further consistent with the wavelength dependency of SHG intensity fit to a two-state model of the two-photon absorption in collagen. We also provide experimental calibration protocols to implement the SHG-CD modality on a laser scanning microscope. We last suggest that the technique has broad applicability in probing a wide range of diseased states with changes in collagen molecular structure.

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Chiral imaging of collagen by second-harmonic generation circular dichroism

Cited by 89 publications

References 42 publications

Collagen chirality and three‐dimensional orientation studied with polarimetric second‐harmonic generation microscopy

Collagen chirality and three‐dimensional orientation studied with polarimetric second‐harmonic generation microscopy

Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils

Wavelength-Dependent Second Harmonic Generation Circular Dichroism for Differentiation of Col I and Col III Isoforms in Stromal Models of Ovarian Cancer Based on Intrinsic Chirality Differences

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