is an experiment aimed at studying the anti-quark distributions of nucleons and nuclei. The experiment uses a 120 GeV/c proton beam extracted from the Main Injector at Fermilab to collide with various solid and cryogenic targets to study a variety of physics topics. The experiment takes advantage of the Drell-Yan process in order to probe specifically the high-x anti-quark distributions of the target nucleus. The acceptance of the spectrometer is tuned to explore the unprecedentedly high Bjorken-x region, thereby extending our knowledge of the anti-quark sea structure of nucleons and nuclei.A new Intensity-Extrapolation method was developed to counter the rate dependence challenge at SeaQuest. Using this method, the ratio of cross sections of liquid deuterium and liquid hydrogen, σ pd (x)/2σ pp (x), was measured in the range 0.1 ≤ x T ≤ 0.45. The results have been found to be consistently above 1 for all values of the measured range of x T . Also, a first look atd(x)/ū(x) is presented. The trend of the data points indicate that the ratio is consistently above 1 for the measured range of x T . These results (when combined with more data) will provide a key input in constraining many theoretical models that attempt to explain the origin of the nucleon sea.Using the same method, the nuclear dependence of the per-nucleon cross section ratio, R pA , of carbon, iron and tungsten versus deuterium studied as a function of x T , p T , andx F are reported. The results for x T are consistent with E772 indicating little nuclear de-I would like to thank Dr. Chuck Brown for his guidance and help. He was always ready for a stimulating physics discussion and never let go of a teachable moment. Working with him was competitive, fun and a great learning experience.My sincere thanks goes to Dr. Kei Nagai for the analysis help he provided. He made my life much easier by helping write, understand, and organize an efficient analysis code. vii I would like to thank Dr. Po-Ju Lin for all the "fun" times at Lab 6 working on repairing and installing the new St 1 drift chamber. Working with 20 micron sense wire was itself difficult but his company made it bearable and fun. I would like to thank Dr. Joshua Rubin for all the inspiring and intellectually stimulating physics discussions. I truly enjoyed our Daddios' diner venting sessions and napkin discussions. I would like to thank Dr. Michelle