Chiral resolution of tryptophan derivatives by CE using canine serum albumin and bovine serum albumin as chiral selectors

Abstract

This work deals with the application of BSA and canine serum albumin (CSA) for enantioseparation of tryptophan derivatives with CE. The aim of this work was the investigation of the influence of different functional groups of tryptophan derivatives on enantioseparation. CSA as a chiral selector was tested to compare its selector properties with those of BSA. The enantiomers of the tryptophan derivatives were separated by adding BSA or CSA to the BGE. The influence of pH, temperature, BSA and CSA concentration and organic modifiers was investigated. It was found that the stereoselectivity for the different tryptophan derivatives is dependent on the albumin species. It turned out that the different functional groups of the derivatives showed a significant influence on stereoselectivity.

An insight into chiral monolithic stationary phases for enantioselective high‐performance liquid chromatography applications

“…In nature, chiral recognition principles are fundamentally relevant to enzymes, plasma proteins, receptors and so on. Several studies reports on the preparation of protein‐based polymer monoliths for chromatographic enantioseparation using different monomers, porogen, and polymerization protocols . In one of these reports, poly(GMA‐ co ‐EDMA) monoliths have been used extensively for post modification approaches due to the presence of reactive epoxy groups susceptible to reaction with various nucleophiles (Table ).…”

Recent advances in amino acid analysis by CE

“…It should be noted that after repeated runs, band-broadening drastically increased and the resolution decreased. To remove adsorbed protein, the capillary was washed with 1 M NaOH after each run [62].…”

Chiral Separations by Capillary Electrophoresis

“…Gübitz's group [106] compared the performance of BSA and canine serum albumin for the separation of tryptophane derivatives. It was found that despite the difference in binding sites, no significant differences in enantioselectivity were found for the investigated substances.…”