Chiral Separation and Determination of Etoxazole Enantiomers in Vegetables by Normal-Phase and Reverse-Phase High Performance Liquid Chromatography

Zhang, Ping; He, Yuhan; Shi, Dongmei; Xu, Yangyang; Yang, Furong; Wang, Jianhao; He, Lin

doi:10.3390/molecules25143134

Cited by 8 publications

(3 citation statements)

References 36 publications

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“…In addition, the retention times increased from (10.5 and 11.9 min) to (11.6 and 13.4 min), respectively. As mentioned in previous studies [ 42 , 43 ], the lower temperature of the column resulted in higher resolutions, longer retention times, and wider peaks. Similarly, the lower temperature of Poroshell 120 CF6 showed a clear advantage on the enantiomeric separation of closantel.…”

Section: Resultsmentioning

confidence: 64%

Analytical Separation of Closantel Enantiomers by HPLC

et al. 2021

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Closantel is an antiparasitic drug marketed in a racemic form with one chiral center. It is meaningful to develop a method for separating and analyzing the closantel enantiomers. In this work, two enantiomeric separation methods of closantel were explored by normal-phase high-performance liquid chromatography. The influences of the chiral stationary phase (CSP) structure, the mobile phase composition, the nature and proportion of different mobile phase modifiers (alcohols and acids), and the column temperature on the enantiomeric separation of closantel were investigated in detail. The two enantiomers were successfully separated on the novel CSP of isopropyl derivatives of cyclofructan 6 and n-hexane-isopropanol-trifluoroacetic acid (97:3:0.1, v/v/v) as a mobile phase with a resolution (Rs) of about 2.48. The enantiomers were also well separated on the CSP of tris-carbamates of amylose with a higher Rs (about 3.79) when a mixture of n-hexane-isopropanol-trifluoroacetic acid (55:45:0.1, v/v/v) was used as mobile phase. Thus, the proposed separation methods can facilitate molecular pharmacological and biological research on closantel and its enantiomers.

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Section: Resultsmentioning

confidence: 64%

Analytical Separation of Closantel Enantiomers by HPLC

et al. 2021

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“…For instance, etoxazole enantiomers can be baseline separated under normalphase and reverse-phase HPLC, and better resolution was observed under normal-phase HPLC. 13 However, the mobile phase under normal-phase conditions is incompatible with mass spectrometry detectors, thus hampering its application in trace analysis. Due to the great solubility of analyte in the mobile phase, polar organic mobile-phase HPLC can achieve fast analysis, which has become increasingly popular in chiral analysis.…”

Section: ■ Hplcmentioning

confidence: 99%

“…Commonly, higher enantiomeric separation resolution was obtained in normal-phase conditions than in reverse-phase conditions. For instance, etoxazole enantiomers can be baseline separated under normal-phase and reverse-phase HPLC, and better resolution was observed under normal-phase HPLC . However, the mobile phase under normal-phase conditions is incompatible with mass spectrometry detectors, thus hampering its application in trace analysis.…”

Section: Hplcmentioning

confidence: 99%

Enantioselective Behaviors of Chiral Pesticides and Enantiomeric Signatures in Foods and the Environment

Wang

Jiang

et al. 2023

J. Agric. Food Chem.

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Unreasonable application of pesticides may result in residues in the environment and foods. Chiral pesticides consist of two or more enantiomers, which may exhibit different behaviors. This Review intends to provide progress on the enantioselective residues of chiral pesticides in foods. Among the main chiral analytical methods, high performance liquid chromatography (HPLC) is the most frequently utilized. Most chiral pesticides are utilized as racemates; however, due to enantioselective dissipation, bioaccumulation, biodegradation, and chiral conversion, enantiospecific residues have been found in the environment and foods. Some chiral pesticides exhibit strong enantioselectivity, highlighting the importance of evaluation on an enantiomeric level. However, the occurrence characteristics of chiral pesticides in foods and specific enzymes or transport proteins involved in enantioselectivity needs to be further investigated. This Review could help the production of some chiral pesticides to single-enantiomer formulations, thereby reducing pesticide consumption as well as increasing food production and finally reducing human health risks.

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