Chiral Separations

Abstract

The main goal of this review is to provide a brief overview of chiral separations to researchers who are versed in the area of analytical separations but unfamiliar with chiral separations. To researchers who are not familiar with this area, there is currently a bewildering array of commercially available chiral columns, chiral derivatizing reagents, and chiral selectors for approaches that span the range of analytical separation platforms (e.g., high-performance liquid chromatography, gas chromatography, supercritical-fluid chromatography, and capillary electrophoresis). This review begins with a brief discussion of chirality before examining the general strategies and commonalities among all of the chiral separation techniques. Rather than exhaustively listing all the chiral selectors and applications, this review highlights significant issues and differences between chiral and achiral separations, providing salient examples from specific classes of chiral selectors where appropriate.

Use of Marfey's reagent and analogs for chiral amino acid analysis: Assessment and applications to natural products and biological systems

“…Several recent reviews on indirect enantioseparation of AAs and related compounds treat the use of MR together with other CDRs [7,[11][12][13]. In continuation of previous reviews/reports on the use of MR for HPLC [14,15] or TLC [16,17] here we focus on newer developments on the use of MR and new variants for the determination of the stereochemistry of protein and non-protein AAs with emphasis on natural products and biological and medical applications.…”

SURMOFs: Liquid-Phase Epitaxy of Metal-Organic Frameworks on Surfaces

“…Chiral separation has important applications in many fields such as in pharmaceutical or agricultural biotechnology [59]. Obliged by the chirality of nature, synthetic pharmaceutics are often chiral compounds.…”

Comparison of polarimetry and crown ether-based HPLC chiral stationary phase method to determine (l)-amino acid optical purity

“…Among various techniques, the liquid chromatographic CSP technique is one of the most accurate, convenient and economical (Luykx & van Ruth, 2008;Lämmerhofer, 2010;Stalcup, 2010;Subramanian, 2007). In particular, liquid chromatographic crown ether-based CSPs have been successfully utilised to separate two enantiomers of various amino acids and to determine the optical purity of various amino acids without derivatisation (Hyun & Choi, 2007).…”

“…Chiral chromatography utilising chiral stationary phases (CSPs) has become an efficient tool to determine enantiomeric purity and to isolate pure enantiomers. CSPs for the liquid chromatographic separation of amino acid enantiomers and their derivatives have been derived from cyclodextrin, macrocyclic antibiotics, designed p-p stacking molecules, cellulose derivatives, proteins, and chiral crown ethers (Lämmerhofer, 2010;Stalcup, 2010;Yu & Wu, 2001).…”