Chitin and chitosan from Brazilian Atlantic Coast: Isolation, characterization and antibacterial activity

Abdel-Rahman, Rasha M.; Hrdina, Radim; Abdel-Mohsen, A.M.; Fouda, Moustafa M.G.; Soliman, A. Y.; Mohamed, Fatma; Kazi, Mohsin; Pinto, Tiago Dinis

doi:10.1016/j.ijbiomac.2015.06.027

Cited by 130 publications

(49 citation statements)

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“…During this treatment, low concentration of acidic solution, which is 15 mL/g of 1.0 N HCl was used to prevent the hydrolysis of chitin structure [17]. Deacetylation process is a conducted, which is to remove the acetyl group in chitin, thus produce chitosan [5]. Bolat et al [18] reported the chitin and chitosan content for freshwater crab were 6.83% and 4.56%, respectively.…”

Section: Results and Discussion Extraction Of Chitin And Chitosanmentioning

confidence: 99%

“…Chitin is available in three different forms, which are α, ß and γ forms. In addition, chitin has a compact structure and strong intra and inters molecular hydrogen bonds [5] that may causes the insolubility in most of the organic solvents, thus limit it in many applications, especially in clinical field. Due to this concern, chemical modification on chitin is performed,…”

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CHITIN AND CHITOSAN EXTRACTION FROM Portunus pelagicus

2017

MJAS

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Isolation of chitin and chitosan from natural sources are widely known as they can be used in many applications. Extraction of chitin and chitosan are carried out in exoskeleton of flower crab, Portunus pelagicus. Chemical treatments such as demineralization and deproteinzation processes are used for chitin extraction, and followed by deacetylation process of chitin, which is to produce its derivatives that known as chitosan. Characterization of chitin and chitosan has been done using Fourier Transform Infrared (FTIR) Spectroscopy, X-ray diffraction (XRD), CHNS elemental analysis and Thermogravimetric analysis (TGA). Preliminary results of FTIR vibrations patterns showed stretching of hydroxyl (O-H), amine (N-H) and carbonyl (C-O) groups indicated the presence of chitin and chitosan. XRD analysis indicates the crystalline nature of chitin and chitosan. The degree of deacetylation of chitin was characterized using CHNS elemental analyzer. The extracted chitosan will be used as reducing agent for silver nanoparticles synthesis in the future studies.Keywords: crustacean, marine invertebrates, bio-polymers, natural reductant Abstrak Pengasingan kitin dan kitosan daripada sumber semulajadi telah dikenali secara meluas kerana dapat digunakan dalam banyak aplikasi. Pengekstrakan kitin dan kitosan dari exoskeleton ketam bunga, Portunus pelagicus telah dilakukan. Rawatan kimia seperti proses demineralisasi dan proses deprotenisasi telah digunakan untuk pengekstrakan kitin dan diikuti dengan proses deasetilasi daripada ktin untuk penghasilan terbitannya yang dikenali sebagai kitosan. Pencirian kitin dan kitosan telah dilakukan dengan menggunakan Spektoskopi Inframerah Transformasi Fourier (FTIR), pembelauan Sinar X (XRD), analisis unsur CHNS dan analisis termogravimetri (TGA). Dapatan awal getaran FTIR menunjukkan regangan bagi kumpulan hidroksil (O-H), amino (N-H) dan karbonil (C-O) menunjukan kehadiran sebatian kitin dan kitosan. Analisis XRD menunjukkan sifat kristal bagi kitin dan kitosan. Pencirian darjah deasetilasi kitin telah dilakukan menggunakan penganalisis unsur CHNS. Ekstrak kitosan akan digunakan sebagai agen penurun bagi sintesis nanopartikel perak untuk kajian masa hadapan.Kata kunci: krustasea, invertebrata marin, bio-polimer, penurun semulajadi. IntroductionPolysaccharides have given many advantages in life science as their unique structure has shown some potential as natural bioactive component. Chitin is the second most abundant of polysaccharides on earth after cellulose that formed a linear chain of acetylglucosamine group. Chitin can be isolated from exoskeleton of crustacean for instance; shrimp shells [1], crab shells [2], fish scales [3], gastropod shell and operculum [4]. Chitin is available in three different forms, which are α, ß and γ forms. In addition, chitin has a compact structure and strong intra and inters molecular hydrogen bonds [5] that may causes the insolubility in most of the organic solvents, thus limit it in many applications, especially in clinical field. Due to this co...

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Section: Results and Discussion Extraction Of Chitin And Chitosanmentioning

confidence: 99%

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CHITIN AND CHITOSAN EXTRACTION FROM Portunus pelagicus

2017

MJAS

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“…The working condition intervals (solution concentration, time, and temperature) for demineralization and deproteinization stages were determined based on the minimum and maximum values from literature data (Abdel‐Rahman et al, ; Kumari & Rath, ; Kumari, Rath, Kumar, & Tiwari, ; Mohammed, Williams, & Tverezovskaya, ; Yen, Yang, & Mau, ).…”

Section: Methodsmentioning

confidence: 99%

Optimization of chitin and chitosan production from shrimp wastes and characterization

Tokatlı

Demirdöven

2017

J Food Process Preserv

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The objectives of this study were to optimize and evaluate chitin and chitosan production conditions from shrimp waste using the response surface methodology and to characterize obtained chitosans. The effects of demineralization and deproteinization conditions on chitin production and deacetylation conditions on chitosan production were investigated. Optimum conditions for chitin production were observed at using 0.73 mol/L hydrochloric acid for 132.61 min at room temperature for demineralization and 0.95 mol/L sodium hydroxide for 75.65 min at 60.49 °C for deproteinization. The obtained chitin was used to optimize chitosan production and two different chitosans were defined as chitosan‐1 and chitosan‐2. Chitosan‐1 was obtained by deacetylation with 40% sodium hydroxide at 120 °C for 300 min, and Chitosan‐2 was obtained by deacetylation with 50% sodium hydroxide at 100 °C for 720 min. The obtained chitosans were characterized by their degree of deacetylation, molecular weight, viscosity, mineral content, whiteness index, and scanning electron microscope micrographs. Practical applications Chitosan, carbohydrate‐based polymer, is derived from crustacean shells. The extraction process of chitin and chitosan from shrimp wastes sourced of different sources needs optimization to obtain high yield chitosan without impurity. Although the production of chitosan by chemical method has been studied earlier, this is the first report on extraction of chitin and chitosan from shrimp waste from the Marmara Sea in Turkey. Shrimp waste from Marmara Sea in Turkey, which has no economic value, can be successfully converted into chitosan. The effective utilization of these wastes would not only eliminate waste but also contribute to the economy as value‐added products.

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“…Chitosan can also bond to metals, which are critical toxic products involved in the growth of microorganisms (22,26). These characteristics of chitosan lead to its possible use in food, cosmetic, pharmaceutical, and biotechnology industries (22,32,36,37).…”

Section: Discussionmentioning

confidence: 99%

<i>In vivo</i> study of antifungal effects of low-molecular-weight chitosan against <i>Candida albicans </i>

Atai

Amini

et al. 2017

Journal of Oral Science

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This study investigated the antifungal effects of low-molecular-weight chitosan solution onCandida albicans in denture stomatitis in comparison with nystatin suspension. This randomized, singleblind clinical trial included 40 patients diagnosed with denture stomatitis. Patients were divided into two groups, wherein one was treated with chitosan and the other with nystatin for 2 weeks. Changes in the erythematous area were recorded during and after treatment. A palatal smear was obtained for each patient before and after treatment to determine the number of blastospores and mycelia of C. albicans. The results were compared using the MannWhitney U test, revealing that the chitosan solution significantly decreased the erythematous surface area, burning sensation, time required for clinical improvement, and number of blastospores and mycelia. The antifungal efficacy of chitosan along with its inherent biocompatibility makes it a promising candidate for use as an antifungal mouthwash.

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Chitin and chitosan from Brazilian Atlantic Coast: Isolation, characterization and antibacterial activity

Cited by 130 publications

References 99 publications

CHITIN AND CHITOSAN EXTRACTION FROM Portunus pelagicus

CHITIN AND CHITOSAN EXTRACTION FROM Portunus pelagicus

Optimization of chitin and chitosan production from shrimp wastes and characterization

<i>In vivo</i> study of antifungal effects of low-molecular-weight chitosan against <i>Candida albicans </i>

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