Chitin and ergosterol content of extraradical and intraradical mycelium of the vesicular-arbuscular mycorrhizal fungus Glomus intraradices

Frey, Beat; Vilariño, A.; Schüepp, H.; Arines, J.

doi:10.1016/0038-0717(94)90263-1

Cited by 72 publications

(33 citation statements)

References 31 publications

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“…The experiments involved detection of the hyphal content of free amino acids and their '°N enrichment. A system using sand-filled hyphal compartments (Frey et al, 1994) was tested for growing external hyphae in amounts sufficient for GC~MS analyses. Sample fatty-acid profiles were also obtained in order to ensure that there was a minimal contamination of the samples by nonmycorrhizal fungi and bacteria.…”

mentioning

confidence: 99%

Nitrogen metabolism of external hyphae of the arbuscular mycorrhizal fungus Glornus intraradices

1996

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SUMMARYThe arbuscular mycorrhizal (AM) fungus Glomus intraradices Schenck and Smith was grown in symbiosis with Cucmnis sativus L. cv. Aminex (Fl hybrid) in mesh hags surrounded by a sand-filled hyphal compartment (HC), allowing only the fungal hyphae to protrude into the HC. The hyphae in the HC were supplied with '°N-labelled NH,* or NOj" after 60 d (expt 1). Following a 48 h labelling period, the sand was removed from the HC and the hyphae extracted. In another experiment (expt 2), the hyphae were extracted from the sand before being incubated in vitro in a nutrient solution containing '""'N-labelled NH^* for 15 h. The hyphal material was incubated in a 0 or 2'5 mM solution of the GOGAT-inhibitor albizzine prior to labelling. In both experiments the hypha! content of free amino acids and fatty acids w^ere measured as well as the amino acid ^°N enrichment.Asparagine was the hyphal amino acid measured in highest concentration followed by glutamine, glutamate, aspartate and alanine. Ornithine, serine and glycine were detected in lower concentrations, but y-ammobutyric acid and citrulline were not detectable. This pattern was the same in both experiments and was unaffected by the type of N applied to the hyphae or by preincubation with albizzine, although the amino-acid concentration decreased considerably in expt 2 compared with expt 1. Both NH^*-N and NO3~-N were assimilated into amino acids but the levels of '°N enrichment following application of NO3~ were much lower than those following application of NH^^ indicating that the latter was more readily assimilated. Albizzine decreased the hyphal amino acid concentration by c. 30 % (without affecting the ^°N enrichment of the individual amino acids) indicating that the AM-fungal hyphae might possess a GS-GOGAT enzyme system for assimilation of inorganic N. The fattyacid profiles (especially phospholipid fatty acids 16:lw5 and 20:5) obtained from the hyphae of G. intraradices showed that contamination of the samples by fungi other than G. intraradices and bacteria was insignificant, and confirmed the usefulness of specific fatty-acid measurement to estimate soil AM-fungal content.

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confidence: 99%

Nitrogen metabolism of external hyphae of the arbuscular mycorrhizal fungus Glornus intraradices

1996

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“…Although we cannot rule out the possible failure in the amplification of Scc's DNA under unknown specific conditions, the DNA of this species might have already been lost from the mycorrhizal roots at this stage. The hypha1 biomass in the AM roots has been estimated by the determination of the contents of phospholipid fatty acids (PLFA) (Olsson et al 1997) and ergosterol (Frey et al 1994) as well as by the conventional microscopic observation (McGonigle et al 1990). The relationship between the DNA content and these biomass indices may differ among species, and even for the same species, the physiological status may change these relationships.…”

Section: Discussionmentioning

confidence: 99%

Semi-quantitative analysis of arbuscular mycorrhizal colonization of onion roots inoculated with single or mixed species based upon PCR-RFLP

Oba

Tawaraya

Saitô

et al. 2002

Soil Science and Plant Nutrition

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“…Combining chromatography with mass spectroscopy extended the use of lipid analysis to the identification and quantification of AM fungi (Schmitz et al 1991). Further fungal metabolites analysed with these techniques include chitin (Frey et al 1994), amino acids (Jin et al 2005) and saccharides (Hooker et al 2007). An important step forward to the understanding of AM fungal metabolism was the introduction of nuclear magnetic resonance (NMR) spectroscopy.…”

Section: Methodologiesmentioning

confidence: 99%

2 Molecular Approaches to Arbuscular Mycorrhiza Functioning

Franken

Waschke

Requena

2012

Fungal Associations

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Chitin and ergosterol content of extraradical and intraradical mycelium of the vesicular-arbuscular mycorrhizal fungus Glomus intraradices

Cited by 72 publications

References 31 publications

Nitrogen metabolism of external hyphae of the arbuscular mycorrhizal fungus Glornus intraradices

Nitrogen metabolism of external hyphae of the arbuscular mycorrhizal fungus Glornus intraradices

Semi-quantitative analysis of arbuscular mycorrhizal colonization of onion roots inoculated with single or mixed species based upon PCR-RFLP

2 Molecular Approaches to Arbuscular Mycorrhiza Functioning

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