Chitin derivatives ameliorate DSS-induced ulcerative colitis by changing gut microbiota and restoring intestinal barrier function

Mei, Zewen; Huang, Xingxi; Zhang, Heng; Cheng, Danyi; Xu, Xin; Fang, Mingyue; Hu, Jutuan; Liu, Yangyang; Liang, Yunxiang; Mei, Yuxia

doi:10.1016/j.ijbiomac.2022.01.049

Cited by 34 publications

(28 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, in another DSS-induced mice model, treatment with chitosan and COS relieved the inflammation caused by ulcerative colitis (UC). The anti-inflammatory effect is characterized by the increased level of anti-inflammatory IL-10, the reduced levels of proinflammatory factors, including IL-1β, IL-6, TNF-α, myeloperoxidase, and inducible nitric oxide synthase (iNOS), as well as the inhibition of the TRL-4/NF-κB/mitogen-activated protein kinase (MAPK) signaling pathway [ 62 ]. Additionally, the administration of COS by oral gavage can reduce proinflammatory cytokines, neutrophil infiltration, and macrophage polarization in the liver of HFD-fed mice by the activation of the adenosine-monophosphate-activated protein kinase (AMPK) signaling pathway [ 53 ].…”

Section: Biological Activities Of Chitosan and Cosmentioning

confidence: 99%

Chitosan and Chitooligosaccharide: The Promising Non-Plant-Derived Prebiotics with Multiple Biological Activities

Guan

Feng

2022

IJMS

View full text Add to dashboard Cite

Biodegradable chitin is the second-most abundant natural polysaccharide, widely existing in the exoskeletons of crabs, shrimps, insects, and the cell walls of fungi. Chitosan and chitooligosaccharide (COS, also named chitosan oligosaccharide) are the two most important deacetylated derivatives of chitin. Compared with chitin, chitosan and COS not only have more satisfactory physicochemical properties but also exhibit additional biological activities, which cause them to be widely applied in the fields of food, medicine, and agriculture. Additionally, due to their significant ability to improve gut microbiota, chitosan and COS are deemed prospective prebiotics. Here, we introduced the production, physicochemical properties, applications, and pharmacokinetic characteristics of chitosan and COS. Furthermore, we summarized the latest research on their antioxidant, anti-inflammatory, and antimicrobial activities. Research progress on the prebiotic functions of chitosan and COS is particularly reviewed. We creatively analyzed and discussed the mechanisms and correlations underlying these activities of chitosan and COS and their physicochemical properties. Our work enriched people’s understanding of these non-plant-derived prebiotics. Based on this review, the future directions of research on chitosan and COS are explored. Collectively, optimizing the production technology of chitin derivatives and enriching understanding of their biological functions will shed more light on their capability to improve human health.

show abstract

Section: Biological Activities Of Chitosan and Cosmentioning

confidence: 99%

Chitosan and Chitooligosaccharide: The Promising Non-Plant-Derived Prebiotics with Multiple Biological Activities

Guan

Feng

2022

IJMS

View full text Add to dashboard Cite

show abstract

Section: The Application Of Different Kinds Of Functional Oligosaccha...mentioning

confidence: 99%

“…For immunity, they inhibited the TLR-4/NF-κB/MAPK signaling pathway activation through downregulating p65 and p38 phosphorylation, suppressed the levels of TNF-α, IL-1β, IL-6 and increased IL-10. 62…”

Section: The Application Of Different Kinds Of Functional Oligosaccha...mentioning

confidence: 99%

“…In addition, a newly published study administered chitin derivatives (CDs) in DSS-induced UC mice, including COS, chitosan (CS), and glucosamine (GlcN), and found that CDs corrected dysbiosis by increasing the abundances of Rikenellaceae_RC9_gut_group, Escherichia-Shigella, Romboutsia, Bacteroides, and Parabacteroides and decreasing the abundances of Anaeroplasma, Ruminococcaceae_UCG-014, and Prevotellaceae_UCG-001. 62 In addition to directly modulating the structure of gut microbiota, COS can also alleviate colitis in other models through a range of mechanisms. They can act by increasing the concentration of SCFAs in the colon, which are important substrates for nourishing colonic epithelial cells.…”

Section: Food and Function Reviewmentioning

confidence: 99%

See 1 more Smart Citation

The role of functional oligosaccharides as prebiotics in ulcerative colitis

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Intestinal immune dysfunction and the imbalance of inflammatory factors are considered to be the direct causes of UC (3), which are mainly manifested in the imbalance of pro-inflammatory and antiinflammatory factors. The species richness, diversity and stability of the intestinal microbiota are also closely related to the activity of intestinal diseases, and the composition of the intestinal microbiota in UC patients is significantly different from that in normal people (4)(5)(6)(7)(8). For example, the expression content of Escherichia coli, Streptococcus, and Bacillus difficile increased, while the expression of Bifidobacterium, Lactobacillus, Clostridium and other probiotics decreased (9), which destroyed the intestinal mucus layer and further aggravated the inflammatory response (10).…”

Section: Introductionmentioning

confidence: 99%

To investigate the effects of artemisinin on inflammatory factors and intestinal microbiota in rats with ulcerative colitis based on network pharmacology

Guo¹,

Li²,

Chen³

et al. 2022

Front. Gastroenterol.

View full text Add to dashboard Cite

ObjectiveTo investigate the therapeutic effect and possible mechanism of artemisinin on ulcerative colitis (UC) induced by sodium glucan sulfate (DSS) in rats based on network pharmacology.MethodsFirst, according to the 3D structure of artemisinin, the effective targets of the active compounds were obtained through the Swissstarge website (www.swisstargetprediction.ch/) and the TargetNet website (http://targetnet.scbdd.com/). With the aid of Genecards (https://www.genecards.org/), OMIM (https://omim.org/), TTD (http://db.idrblab.net/ttd/) to obtain effective targets of disease. The disease gene-drug target network was constructed by extracting the intersection targets of the two, and the visualization operation and analysis were performed by using Cytoscape 3.7.2. Gene function enrichment analysis and pathway analysis were performed on the intersection targets with the help of R language software. Autidock Vina was used for molecular docking of artemisinin to key targets. Then, 40 male Wistar rats were randomly divided into normal group, model group, mesalazine group (0.315 g/kg·d) and artemisinin group (0.1 g/kg·d), with 10 rats in each group. Except for the normal group, the rats in the other groups were given 3.5% DSS solution freely for 10 days to replicate the UC model. After the successful modeling, the rats were given intragastric administration. The normal group and the model group were given the same amount of 0.9% normal saline, once a day, for 14 days. The general condition of the rats was recorded every day and the disease activity index (DAI) score was performed. After the administration, the colonic mucosal damage index (CMDI) was scored, the histopathological changes of the colon were observed by HE staining, and the levels or activities of serum CRP, TNF-α, MDA, SOD, HIF-1α and T-AOC were detected by ELISA, and fecal and intestinal microbiota of rats were detected by 16S rDNA sequencing.ResultsNetwork pharmacology shows that, there were 98 key targets of artemisinin screening, 4853 effective targets of UC, and 43 intersection targets for artemisinin and UC, involving 48 signaling pathways. The molecular docking results showed that the binding energies of the key proteins to artemisinin were less than -5.0 kJ·mol-1, and the binding energy of PTGS2 NOS3 to artemisinin was the best. Animal experiments have shown that, Compared with the model group, the DAI and CMDI scores of the artemisinin group and the mesalazine group decreased, the levels and activities of serum CRP, TNF-α, MDA and HIF-1α decreased, the levels and activities of SOD and T-AOC increased, the abundance and diversity of inteatinal microbiota increased, and the abundance of p-Acidobacteria, p-Chloroflexi, p-Gemmatimonadetes, p-Nitrospirae in artemisinin group increased (P＜0.05), and there was no significant change in others.ConclusionArtemisinin intervenes with UC through key target proteins such as PTGS2 and ESR1, and involves various biological processes such as inflammation and intestinal microbiota, revealing that molecular basis of artemisinin in the treatment of UC. Artemisinin is effective in improving the symptoms of UC rats, and its mechanism may be to relieve oxidative stress response by inhibiting inflammation, thus promoting intestinal mucosal repair. The regulatory effect on intestinal microbiota needs to be further studied.

show abstract

Chitin derivatives ameliorate DSS-induced ulcerative colitis by changing gut microbiota and restoring intestinal barrier function

Cited by 34 publications

References 66 publications

Chitosan and Chitooligosaccharide: The Promising Non-Plant-Derived Prebiotics with Multiple Biological Activities

Chitosan and Chitooligosaccharide: The Promising Non-Plant-Derived Prebiotics with Multiple Biological Activities

The role of functional oligosaccharides as prebiotics in ulcerative colitis

To investigate the effects of artemisinin on inflammatory factors and intestinal microbiota in rats with ulcerative colitis based on network pharmacology

Contact Info

Product

Resources

About