Chitinase, chitosanase and β-1,3-glucanase activities in Allium and Pisum roots colonized by Glomus species

Dumas‐Gaudot, Eliane; Grenier, Jean; Furlan, V.; Asselin, Alain

doi:10.1016/0168-9452(92)90203-x

Cited by 68 publications

(31 citation statements)

References 29 publications

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“…Immunological assays were performed aiming to check if the differentially expressed proteins cross-reacted with antibodies raised against proteins induced by plant-root pathogen interactions, but the results were not conclusive [29]. Later, leek, onion, and pea roots were also analyzed by native PAGE, but resolution was improved by coupling the native gels to denaturing SDS gels (SDS-PAGE), which resulted in twodimensional native/SDS-PAGE gels [30]. In the same study, differentially expressed proteins were shown to be associated to chitinase, chitosanase and beta-1,3-glucanase activities, enzymes previously reported as related to plant response to pathogen attack.…”

Section: Protein Separation Methods: Improving Resolutionmentioning

confidence: 99%

“…As simple as this extraction method may be, it was shown to be fairly efficient for extracting readily soluble proteins from tissues that do not have high contents of non-proteinaceous interfering substances, like polysaccharides, lipids, or proteases. Such studies were initially performed on tobacco, onion, leek and pea plants [29,30]. Very often plant tissue samples contain proteolytic enzymes that are released into the buffer solution during cell rupture.…”

Section: First Steps On Am Symbiosis Protein Studiesmentioning

confidence: 99%

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Proteomic studies of arbuscular mycorrhizal associations

Couto¹,

Lovato²,

Wipf³

et al. 2013

ABC

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Arbuscular mycorrhizal (AM) fungi are soil-borne microorganisms forming mutualistic associations with the vast majority of land plants, including most agricultural relevant crops. In this association the plant provides the fungus with plant photosynthates allowing it to complete its life cycle, while the fungus provides the plant with mineral nutrients, mainly phosphorus and can also help the plant to tolerate biotic and abiotic stresses. In regard to these benefits there is growing interest on the use of AM fungi to improve productivity and sustainability in agricultural systems. AM fungi and their interactions with plants have been extensively studied using proteomic techniques, but some difficulties have been faced. 1) Little is known about the AM fungal typical protein repertoire because it is currently impossible to grow AM fungi in pure axenic cultures; 2) Plant tissues often contain high amounts of interfering substances that make protein extraction for the study of AM interactions a difficult procedure; 3) Most nutrient exchanges between AM fungi and their host plants involve participation of membrane proteins, still poorly resolved in most separation techniques. Finally, 4) the formation of the arbuscule is an asynchronous process, making it difficult to distinguish which proteins are essential in the early or late stages of AM associations. In this review we present a historical summary of how these difficulties have been overcome by technological advances in proteomics and we discuss current and future trends in the study of the proteins involved in AM interactions.

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Section: Protein Separation Methods: Improving Resolutionmentioning

confidence: 99%

Section: First Steps On Am Symbiosis Protein Studiesmentioning

confidence: 99%

Proteomic studies of arbuscular mycorrhizal associations

Couto¹,

Lovato²,

Wipf³

et al. 2013

ABC

Self Cite

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“…Moreover, onion association with AMF can enhance plant height, expansion of leaf area, chlorophyll content, plant biomass as well as dry mass and diameter of bulbs (Bolandnazar et al 2007). In addition, mycorrhizal symbiosis can improve the defences of plants against bacterial and fungal pathogens due to increased activity of chitinase (Dumas-Gaudot et al 1992), higher antimicrobial activity and enhanced concentration of phenolic compounds in roots (Lokhandwala et al 2014). Moreover, mycorrhizal association in onion can also increase both the uptake of nutrients in dry soils (Azcón, Tobar 1998) and yield, whose magnitude depend on the type of fungus inoculated to plants (Afek et al 1990).…”

Section: Farming Practicesmentioning

confidence: 99%

Interactions among genotype, environment and agronomic practices on production and quality of storage onion (Allium cepa L.) - A review

Sękara¹,

Pokluda²,

Vacchio³

et al. 2017

Hortic. Sci.

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Sekara A., Pokluda R., Del Vacchio L., Somma S., Caruso G. (2017) The aim of this review is a wide description of the relationships between growing conditions and bulb yield and quality of onion (Allium cepa L.), focused particularly on long-day cultivars suitable for storage. Marketable yield decreases according to the reduction of crop length caused by the increase of growth temperature. The nutritive requirements of storage onion are highest during the vegetative growth. The application of humic substances and the inoculation of mycorrhizae may enhance bulb growth and quality, mainly under stress conditions. Onion is a slow-growth, shallowrooted crop with non-shading habitus and therefore its productivity is highly dependent on water availability in the soil, proper fertilization and weed control. The shelf-life of onion bulbs is a genetic trait, improvable by efficient crop and post-harvest management, and adequate conditions of bulb storage. The quality of storage onion bulbs is ascribed to several indicators, such as thiosulfonates, pyruvic acid, soluble solids, sugars, and many other biological compounds. This review is also focused on onion quality as affected by the interactions among genotype, environment, farming practices and post-harvest management.

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“…Using antibodies against a bean basic chitinase, antigenic proteins were localized in vacuoles, extracellular spaces and degenerating arbuscules of mycorrhizal roots of leek (Spanu et al, 1989). Dumas-Gaudot et al (1992) observed induction of specific acidic chitinase isoforms in AM roots of leek, onion and pea. Additionally, mycorrhiza-specific acidic chitinases have been detected in pea and tomato using native PAGE (Dassi et al, 1996 ;Pozo et al, 1996).…”

Section: mentioning

confidence: 99%

Spatial distribution of chitinases and β‐1,3‐glucanase transcripts in bean arbuscular mycorrhizal roots under low and high soil phosphate conditions

Lambais

Mehdy

1998

New Phytologist

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Arbuscular mycorrhizal (AM) fungi extensively colonize the root cortex under low-soil-phosphate (P) conditions, whereas infection is limited under high-P conditions. Fungal growth under both P conditions might be influenced by plant defence-related gene expression. In this study, we used in situ hybridization methods to compare the cellular localization of three defence-related mRNAs in non-infected bean roots and in relation to fungal infection units. In non-infected and infected roots, mRNAs encoding acidic and basic endochitinases were generally most abundant in the vascular cylinder. High-P-grown mycorrhizal roots showed localized accumulation of the acidic endochitinase mRNA in cortical cells containing arbuscules and in their immediate vicinity (one to five cell layers). The pattern of accumulation of the basic endochitinase mRNA was not affected by P or AM fungal infection. At the low P concentration, the β-1,3-glucanase mRNA accumulated predominantly in the vascular cylinder of noninfected roots. Suppression of β-1,3-glucanase mRNA accumulation in these tissues was observed in non-infected roots at the high-P and in mycorrhizal roots at both P concentrations. The observed suppression extends at least several mm from fungal infection units, characterizing a systemic effect. Beta-1,3-glucanase mRNA accumulated also around a number of cortical cells containing arbuscules only at the low P concentration. The localized accumulations of the endochitinase and β-1,3-glucanase mRNAs suggest that the encoded proteins might be involved in the control of intraradical fungal growth.

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Chitinase, chitosanase and β-1,3-glucanase activities in Allium and Pisum roots colonized by Glomus species

Cited by 68 publications

References 29 publications

Proteomic studies of arbuscular mycorrhizal associations

Proteomic studies of arbuscular mycorrhizal associations

Interactions among genotype, environment and agronomic practices on production and quality of storage onion (Allium cepa L.) - A review

Spatial distribution of chitinases and β‐1,3‐glucanase transcripts in bean arbuscular mycorrhizal roots under low and high soil phosphate conditions

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