Chitosan and its derivatives for gene delivery

Saranya, N; Moorthi, A.; Sekaran, Saravanan; Devi, Madhabi; Selvamurugan, N.

doi:10.1016/j.ijbiomac.2010.11.013

Cited by 228 publications

(109 citation statements)

References 110 publications

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“…Unfortunately, PLGA has a negative charge, which limits its interaction with DNA/RNA, so cationic compounds have been combined with this polymer in order to condense genetic material efficiently. 17,18 For example, chitosan, a polysaccharide used widely in the pharmaceutical field because of its biocompatibility, biodegradability, safety, and mucoadhesive properties, 19,20 has been used in combination with PLGA in order to increase transfection efficiency. 21,22 In 2007, Nafee et al investigated the variation in physicochemical parameters of PLGA/high molecular weight chitosan nanoparticles (produced by the emulsion-diffusionevaporation technique) as a function of the proportions of compounds contained therein.…”

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confidence: 99%

Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes

Cosco

Federico

Maiuolo

et al. 2014

IJN

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The aim of this study was the evaluation of the effects of two emulsifiers on the physicochemical and technological properties of low molecular weight chitosan/poly (D,L-lactide-co-glycolide) (PLGA) nanoplexes and their transfection efficiency. Nanospheres were prepared using the nanoprecipitation method of the preformed polymer. The mean diameter and surface charge of the nanospheres were investigated by photocorrelation spectroscopy. The degree of binding of the plasmid with the nanoplexes was qualitatively and quantitatively determined. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) testing was performed using HeLa, RPMI8226, and SKMM1 cell lines. Flow cytometry and confocal laser scanning microscopy were used to determine the degree of cellular transfection and internalization of the nanoplexes into cells, respectively. The nanoplexes had a positive zeta potential, and low amounts of PLGA and poloxamer 188 showed a mean colloidal size of ~200 nm with a polydispersity index of ~0.14. The nanoplexes had suitable entrapment efficiency (80%). In vitro experiments showed that the colloidal nanodevices did not induce significant cytotoxicity. The nanoplexes investigated in this study could represent efficient and useful nonviral devices for gene delivery. Use of low amounts of PLGA and poloxamer 188 enabled development of a nanosphere able to transfect cells efficiently. These nanosystems are a helpful platform for delivery of genetic material while preserving therapeutic efficacy.

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confidence: 99%

Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes

Cosco

Federico

Maiuolo

et al. 2014

IJN

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“…Factors including the degree of deacetylation, molecular weight, and charge of chitosan, and the media pH are important in determination of the transfection efficiency of polyplexes containing chitosan and DNA (Huang, et al, 2005, Ishii, et al, 2001, Lavertu, et al, 2006. The increased degree of deacetylation resulted in an increased level of DNA binding ability, and high transgene expression due to higher charge density along the chain (Kiang, et al, 2004, Lavertu, et al, 2006, Saranya, et al, 2011. The effect of the molecular weight of chitosan on complex formulation with DNA can be attributed to the chain entanglement effect (Kiang, et al, 2004).…”

Section: Cyclodextrinmentioning

confidence: 99%

Medical Polymer-Based Gene Therapy

Jiang¹,

Kim²,

Cho³

et al. 2011

Non-Viral Gene Therapy

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“…Chitosan has various biological properties including antimicrobial properties [12], antioxidant properties [13], and anti-inflammatory properties [14]. Chitosan is also mucoadhesive, making it highly suitable for gene delivery to epithelium including the lungs and gastrointestinal tract [15][16][17]. Chitosan has found use in novel applications such as vaccine and peptide delivery, in addition to its use in tissue engineering [2,6,18].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Biological Evaluation of New Imine- and Amino-Chitosan Derivatives

et al. 2015

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N-substituted chitosan derivatives were synthesized through condensation with a number of selected aryl and heteroaryl aldehydes. The synthesis of the amino-derivatives has been carried out by reductive amination with sodium borohydride as reducing agent. Their structures were characterized by (FT-IR, 1 HNMR, and XRD). The antimicrobial activity of Chitosan Schiff's base (CSB) derivatives were investigated against four types of bacteria and two crop-threatening pathogenic fungi, and the results indicated that the antibacterial and antifungal activities of the investigated derivatives are very promising. Additionally, different concentrations of the triazolo-Schiff's base derivative 3c were used for cytotoxicity screening against Human Breast Adenocarcinoma Cells (MCF-7), Human Colon Carcinoma Cells (HCT-116), and Human Hepatocellular Liver Carcinoma Cells (HepG-2), and the obtained data revealed that the examined compounds have an excellent cell growth inhibitory effects on the cell lines as compared to standard.

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Chitosan and its derivatives for gene delivery

Cited by 228 publications

References 110 publications

Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes

Physicochemical features and transfection properties of chitosan/poloxamer 188/poly(D,L-lactide-co-glycolide) nanoplexes

Medical Polymer-Based Gene Therapy

Synthesis and Biological Evaluation of New Imine- and Amino-Chitosan Derivatives

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