Chitosan-coated silica beads as immobilized metal affinity support for protein adsorption

pH-Sensitive Nitroxide Radicals for Studying Inorganic and Organo-Inorganic Materials and Systems

“…Using an organic polymer (e.g., cellulose) as a substrate also has advantages in the sorption of metal ions (Corma et al, 2007). Metal-containing hybrid organo-inorganic materials can also be used as antibacterial composites (Mei et al, 2009), as sorbents of proteins (Shi et al, 2003), and as pervaporation membranes (Varghese et al, 2010). Nanostructured metal oxides, which are distinguished by extremely developed surface and porosity of particles, are new promising materials for different fields of science and technology, especially, for heterogeneous catalysis and chemistry of adsorption phenomena (Zakharova et al, 2005).…”

Chitosan microspheres as immobilized dye affinity support for catalase adsorption

“…By attaching ␤-cyclodextrin onto the chitosan gel, Harsa and Furusaki [17,18] [19,20] prepared the cross-linking chitosan coated silica beads as an affinity chromatography matrix of ovealbumin and trypsin. Moreover, chitosan-coated silica beads were used as immobilized metal affinity support for protein adsorption [21,22].…”

Immobilised Metal Affinity Chromatography (Imac) Beads for Lysozyme Separation: Synthesis and Characterization Study

“…Preparation of Chitosan-coated silica beads (CTS-SiO 2 ) was according to Shi et al [8] with some modification. Chitosan powder (5 g) was added into 250 ml of 1M acetic acid and dissolved by magnetic stirring for several minutes at room temperature and finally, the prepared chitosan solution was stored in a Schott bottle at room temperature.…”

“…However, chitosan required a cross-linker to modify chemical structure and textural properties by linking at the amine or the hydroxyl sites. Glutaraldehyde (GTA) is the best chosen cross-linker for chitosan which has a porous network that could improve adsorption properties and helped to form quaternary structure on the surface of the materials, thus increase the content of protonated amino groups [7] as well as improved the chemical stability of chitosan in acidic solution [8]. Practically, IMAC is depend on the formation of coordinate bonds between immobilized metal ions such as Cu , and Fe 2+ with the functional groups of exposed amino acids on the surface of the target protein [9].…”