Chitosan derivative functionalized carbon nanotubes as carriers for enzyme immobilization to improve synthetic efficiency of ethyl caproate

Ji, Suping; Liu, Wei; Su, Shuyi; Gan, Caifang; Jia, Chengsheng

doi:10.1016/j.lwt.2021.111897

Cited by 20 publications

(12 citation statements)

References 41 publications

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“…The immobilized PPL retained 45% of its initial activity after 8 h of incubation at 40 °C , while the free PPL retained 26% under similar conditions. Indeed, other chitosan-based scaffolds support the above results [45][46][47]. The carefully designed immobilization carriers stabilize the biocatalytic system and offer a microenvironment that protects the enzyme from denaturation and, hence, activity loss.…”

Section: Thermal Stabilitymentioning

confidence: 65%

3D Printed Polylactic Acid (PLA) Well Plates for Enzyme Inhibition Studies: The Case of Pancreatic Lipase

STAMATIS¹,

Gournis²,

POLYDERA³

et al. 2022

Catal Res

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3D printed PLA has already been demonstrated for several biotechnological applications, including enzymes immobilization. The prerequisites for an efficient screening assay include using small volumes of reagents, low cost, and rapid screening of large numbers of compounds and extracts. Hence, assays based on microtiter plates are predominant. Thus, designing and fabricating scaffolds on a similar scale, which could serve as immobilization carriers, and their recruitment in inhibitors screening studies is of great significance, adding both enzyme stability and reuse potentiality of the biocatalytic system in assay merits. In this work, pancreatic lipase was immobilized on 3D-printed PLA microwells for enzyme inhibitor screening. XPS analysis demonstrated the successful modification of the PLA scaffolds. The immobilized enzyme displayed high levels of operational, thermal, and storage stability under the tested conditions. The IC<sub>50</sub> values for PPL inhibition were calculated for Orlistat, a model lipase inhibitor, and olive leaf extract, a promising natural compound. This is the first study reporting the use of 3D-printed PLA wells with an immobilized enzyme for inhibitor screening assay.

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Section: Thermal Stabilitymentioning

confidence: 65%

3D Printed Polylactic Acid (PLA) Well Plates for Enzyme Inhibition Studies: The Case of Pancreatic Lipase

STAMATIS¹,

Gournis²,

POLYDERA³

et al. 2022

Catal Res

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“…CNT and CS derivatives are conjugated by either π–π stacking interactions [ 167 ] or electrostatic interactions when the CNT surface is modified with carboxylic acids [ 145 ] ( Figure 10 ). Interpenetration between cross-linked CS derivatives and CNT was also reported [ 145 , 146 ].…”

Section: Combination Of Covalent and Non-covalent Modificationsmentioning

confidence: 99%

Chitosan Functionalization: Covalent and Non-Covalent Interactions and Their Characterization

2021

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Chitosan (CS) is a natural biopolymer that has gained great interest in many research fields due to its promising biocompatibility, biodegradability, and favorable mechanical properties. The versatility of this low-cost polymer allows for a variety of chemical modifications via covalent conjugation and non-covalent interactions, which are designed to further improve the properties of interest. This review aims at presenting the broad range of functionalization strategies reported over the last five years to reflect the state-of-the art of CS derivatization. We start by describing covalent modifications performed on the CS backbone, followed by non-covalent CS modifications involving small molecules, proteins, and metal adjuvants. An overview of CS-based systems involving both covalent and electrostatic modification patterns is then presented. Finally, a special focus will be given on the characterization techniques commonly used to qualify the composition and physical properties of CS derivatives.

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“…However, several bottlenecks, such as low operational stability, reusability and high cost, restricted the development and large-scale application of enzymes. 8,9 One promising strategy was selecting suitable carrier material to immobilize enzymes by different protocols in order to create biocatalytic nanoreactors with high performance and good stability. 10−14 Not only that, but now, proper immobilization may improve other properties of enzymes such as activity, specificity, selectivity, reagent tolerability, and even purity.…”

Section: ■ Introductionmentioning

confidence: 99%

“…11,17 The socalled interfacial activation and the higher binding capacities of lipase could be achieved by anchoring lipases on some appropriate supports. The high specific surface area, 8,9,18,19 proper bionanointerface composition, 20,21 and excellent recyclability were also necessarily required for the ideal carrier materials. 22 Hollow carbon sphere nanoreactors allowed large cargoes of guest molecules to be inner loaded and accelerated the accumulation of reactants, even promoting the conversion of the intermediate in the void space.…”

Section: ■ Introductionmentioning

confidence: 99%

“…However, several bottlenecks, such as low operational stability, reusability and high cost, restricted the development and large-scale application of enzymes. , One promising strategy was selecting suitable carrier material to immobilize enzymes by different protocols in order to create biocatalytic nanoreactors with high performance and good stability. − Not only that, but now, proper immobilization may improve other properties of enzymes such as activity, specificity, selectivity, reagent tolerability, and even purity. ,, Immobilizing lipase on the relatively hydrophobic supports via interfacial activation was one of effective methods, which induce the conformational changes of the “lid” domain. , The so-called interfacial activation and the higher binding capacities of lipase could be achieved by anchoring lipases on some appropriate supports. The high specific surface area, ,,, proper bionanointerface composition, , and excellent recyclability were also necessarily required for the ideal carrier materials . Hollow carbon sphere nanoreactors allowed large cargoes of guest molecules to be inner loaded and accelerated the accumulation of reactants, even promoting the conversion of the intermediate in the void space. − In an inspiring manner, the hollow mesoporous nanoparticles were undoubtedly an ideal enzyme-encapsulated material for biocatalysis .…”

Section: Introductionmentioning

confidence: 99%

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Hollow Mesoporous Carbon-Based Enzyme Nanoreactor for the Confined and Interfacial Biocatalytic Synthesis of Phytosterol Esters

Zhang

Hou

Zhao

et al. 2023

J. Agric. Food Chem.

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Rationally designing carriers to obtain efficient and stable immobilized enzymes for the production of food raw materials is always a challenge. In this work, hollow cube carbon (HMC) as a carrier of Candida rugosa lipase (CRL) was prepared to construct a Pickering interfacial biocatalysis system, which was applied to biphasic biocatalysis. For comparison, the nonporous carbon (HC) and porous MoS 2 (HMoS 2 ) were also designed. On these grounds, p-NPP and linolenic acid were selected as the representative substrates for hydrolysis and esterification reactions. Under the optimal conditions, the protein loading amount, specific activity, and expressed activity of CRL immobilized on HMC (HMC@CRL) were 167.2 mg g −1 , 5.41 U mg −1 , and 32.34 U/ mg protein, respectively. In the "oil−water" biphase, the relative hydrolytic activity of HMC@CRL was higher than that of HC@ CRL, HMoS 2 @CRL, and CRL by 50, 68, and 80%, respectively, as well as itself in one phase. Compared to other reports (1.13%), HMC@CRL demonstrated a satisfactory hydrolysis rate (3.02%) and was the fastest among all other biocatalysts in the biphase. Moreover, compared with the free CRL in one-phase system, the Pickering interfacial biphasic biocatalyst, HMC@CRL, exhibited a higher esterification rate (85%, 2.7-fold enhancement). Therefore, the HMC@CRL nanoreactors had more optimal performance in the field of biomanufacturing and food industry.

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Chitosan derivative functionalized carbon nanotubes as carriers for enzyme immobilization to improve synthetic efficiency of ethyl caproate

Cited by 20 publications

References 41 publications

3D Printed Polylactic Acid (PLA) Well Plates for Enzyme Inhibition Studies: The Case of Pancreatic Lipase

3D Printed Polylactic Acid (PLA) Well Plates for Enzyme Inhibition Studies: The Case of Pancreatic Lipase

Chitosan Functionalization: Covalent and Non-Covalent Interactions and Their Characterization

Hollow Mesoporous Carbon-Based Enzyme Nanoreactor for the Confined and Interfacial Biocatalytic Synthesis of Phytosterol Esters

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