Chitosan: effect of a new chelating agent on the microhardness of root dentin

Pimenta, Josilaine Amaral; Zaparolli, Danilo; Pécora, Jesus Djalma; Filho, Antônio Miranda da Cruz

doi:10.1590/s0103-64402012000300005

Cited by 81 publications

(72 citation statements)

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“…The type of interaction that occurs depends on the involved ions, the chemical structure of chitosan and the pH of the solution (23). Pimenta et al (24) reported that the 0.2% chitosan solution (pH 3.2) has a capacity of reducing dentin microhardness similar to that of 15% EDTA (pH 7.25).…”

Section: Discussionmentioning

confidence: 99%

Time-dependent effects of chitosan on dentin structures

et al. 2012

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Complete debridement with smear layer removal are essential measures for achieving a successful outcome of root canal treatment. The aim of this study was to evaluate the effects of chitosan at different concentrations on the removal of the smear layer and on dentin structure after 3 and 5 min of application. Twelve recently extracted maxillary canine teeth were instrumented using the crown-down technique and irrigated with 1% sodium hypochlorite. The specimens were distributed according to the time and concentration of the final irrigating solution: G1: 0.1% chitosan for 3 min; G2: 0.2% chitosan for 3 min; G3: 0.37% chitosan for 3 min; G4: 0.1% chitosan for 5 min; G5: 0.2% chitosan for 5 min; G6: 0.37% chitosan for 5 min. All samples were prepared for SEM analysis. G1 exhibited removal of the smear layer, but not the smear plugs. G2 showed visible and open tubules with slight erosion of the peritubular dentin. Cleaning in G3 was similar to that in G2, however, the erosive effect was greater. There was expansion of the diameter of the tubules in G4; and in G5 and G6, there was severe erosion with deterioration of dentin surface. In conclusion, 0.2% chitosan for 3 min appeared to be efficient for removing the smear layer, causing little erosion of dentin.

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Section: Discussionmentioning

confidence: 99%

Time-dependent effects of chitosan on dentin structures

et al. 2012

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“…Complexes of chitosan and metal ions are most probably formed by the mechanisms of adsorption, ion exchange and chelation [40,43]. Pimentaet al [44] reported that the 0.2% chitosan solution (pH=3.2) has a capacity to reduce dentin microhardness similar to that of 15% EDTA (pH=7.25) representing similar demineralization potential. The effect of 0.2% chitosan applied for 3 minutes was the most effective for removing the smear layer and smear plug with minimal erosive effect on dentin substrate as suggested previously in the study of Silva et al [38].…”

Section: Discussionmentioning

confidence: 99%

Adhesive strength of self-adhesive resins to lithium disilicate ceramic and dentin: effect of dentin chelating agents

2014

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The aim of this study was to test the effect of different chelating agents on microtensile bond strength (MTBS) of self-adhesive resin cements to dentin. The occlusal surfaces of extracted human mandibular molars (N = 80) were cut horizontally to expose sound dentin. The teeth were randomly divided into four groups (n = 20 per group) and dentin surfaces were conditioned according to one of the following methods: group C: no treatment (control group); group CH: 0.2 % chitosan; group E: 17 % ethylene diamine tetra acetic acid (EDTA) and group P: 25 % polyacrylic acid (PAA). Lithium disilicate glass ceramic (e.max CAD) blocks were cemented to conditioned dentin surfaces with self-adhesive cements (RelyX Unicem or Clearfil SA) and photo-polymerized. Specimens were stored in distilled water at 37°C for 24 h and thermocycled for 6,000 times. The beams were obtained from bonded ceramic-cement-tooth assemblies and were subjected to the MTBS test (1 mm/min). Failure types were analyzed and selected beams were examined under scanning electron microscope. Data (MPa) were analyzed using two-way ANOVA and Tukey's test (P < 0.05). While cement type significantly affected the MTBS results (P < 0.05), no significant difference was observed between the dentin chelating agents (P = 0.785). Interaction terms were not significant (P = 0.114). Control group with no dentin conditioning presented significantly lower results with both cements (RelyX Unicem: 8.1 ± 1.9(a), Clearfil SA: 8 ± 1.6(a)) than those of conditioned groups (19.3 ± 4.2(b)-24.5 ± 5.2(b)) (P < 0.05). Failure types were predominantly adhesive in all groups. Chitosan (2 %), EDTA (17 %) or PAA (25 %) could all be used as dentin chelating agents in conjunction with self-adhesive resin cements tested. Abstract:The aim of this study was to test the effect of different chelating agents on microtensile bond strength (MTBS) of self-adhesive resin cements to dentin. The occlusal surfaces of extracted human mandibular molars (N=80) were cut horizontally to expose sound dentin. The teeth were randomly divided into 4 groups (n=20 per group) and dentin surfaces were conditioned according to one of the following methods: Group C: No treatment (Control group); Group CH: 0.2% chitosan; Group E: 17% ethylene diamine tetra acetic acid (EDTA) and Group P: 25% polyacrylic acid (PAA). Lithium disilicate glass ceramic (e.max CAD) blocks were cemented to conditioned dentin surfaces with self-adhesive cements (RelyX Unicem or Clearfil SA) and photo-polymerized.Specimens were stored in distilled water at 37 o C for 24 hours and thermocycled for 6000 times.The beams were obtained from bonded ceramic-cement-tooth assemblies and they were subjected to the MTBS test (1 mm/min). Failure types were analyzed and selected beams were examined under scanning electron microscope. Data (MPa) were analyzed using two-way ANOVA and Tukey's test (P<0.05). While cement type significantly affected the MTBS results (P<0.05), no significant difference was observed between the dentin chelating agents (...

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“…Apesar de ambas as substâncias atuarem de formas distintas na quelação do cálcio, pode-se supor, pelos resultados, que a ação de ambas foi equivalente na remoção da lama dentinária, como verificado em outras condições [15][16][17] . A solução de quitosana tem pH mais ácido por ser diluida em ácido acético 1%, e sua ação se baseia em duas teorias: a ligação do grupo amino da cadeia de quitosana se liga ao íon metálico (cálcio), ou seu grupo amino é envolvido na ligação, e por sua vez o íon metálico se ancora ao grupo amino 25 .…”

Section: Methodsunclassified

“…Uma delas é a quitosana, um polissacarídeo natural, abundante na natureza, ecologicamente correto, com propriedades de biocompatibilidade, biodegrabilidade, bioadesão e atoxidade frente ao organismo humano. Tem a capacidade de desmineralizar o tecido dentinário de forma semelhante ao EDTA, proporcionando limpeza das paredes dentinárias por meio da remoção da lama dentinária, com ação equivalente dos ácidos cítrico e diaminotetracético, mas com a vantagem de ser utilizado em pequenas concentrações e por curto período de tempo [14][15][16][17] . Entre as diversas aplicações, a quitosana tem sido utilizada em tratamentos distintos na odontologia, por inibir a formação de biofilmes 18 , pela capacidade de compor scaffolds de alta resistência 19 , por sua ação cicatrizante, anti-inflamatória e antimicrobiana 18,20,21 .…”

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Avaliação da Resistência de União Entre Resina Composta e Substrato Dentinário Exposto à Quitosana

et al. 2017

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ResumoEm endodontia, indicam-se substâncias quelantes para a remoção da lama dentinária. O objetivo deste estudo foi avaliar, in vitro, por meio do microcisalhamento, a resistência de união da resina composta com superfícies dentinárias tratadas com EDTA (17%) e quitosana (0,2%). De 30 coroas de dentes bovinos foram removidas as paredes palatina, mesial e distal, expondo a superfície dentinária da parede vestibular da câmara coronária, que abrasionada produziu a camada de esfregaço. Após, a superfície foi tratada, estabelecendo-se 3 grupos (n=10): G1 (controle) com aplicação de água destilada (1min); G2 com aplicação de EDTA (3 min.), e lavagem com água destilada (1 min.); e G3 com aplicação de quitosana (3 min.), lavagem com água destilada (1 min.). Na sequência foram aplicados 2 ml de NaOCl (2,5%), 2 ml de água destilada (1 min), armazenando-as em água destilada (37 °C). Retiradas da água destilada (24 h), fixaram -se 3 tubos Tygon (1mmX1mm) na dentina após o condicionamento local com sistema adesivo convencional de 2 passos. Preenchidos com resina composta e após fotoativação, os tubos foram removidos obtendo-se cilindros que foram submetidos ao microcisalhamento em máquina de ensaio universal. Os resultados de resistência de união (MPa) foram submetidos a Análise de Variância e Tukey, com nível de significância 5%. Observou-se que a resistência de união de G2 e G3 foram equivalentes estatisticamente (p>0,05), porém significativamente maiores que em G1 (p<0,05). Conclui-se que quitosana e EDTA promovem resistência de união semelhante nas condições testadas.Palavras-chave: Camada de Esfregaço. Dentina. Endodontia. AbstractIn endodontics, chelating substances are indicated for smear layer removal. This study evaluated, by micro-shear, the bond strength between composite resin and dentin surfaces treated with EDTA (17%) and chitosan (0.2%). The palatal, mesial and distal walls were removed from 30 crowns of bovine teeth, to expose buccal dentin of coronary chamber, whose abrasion produced the smear layer. The surface was treated, considering 3 groups (n = 10): G1 (control) distilled water (1min); G2 EDTA application (3 min), and distilled water (1 min); and G3 chitosan application (3 min.), distilled water (1 min.). All groups received 2 ml of NaOCl (2.5%), 2 ml of distilled water (1 min), and then stored in distilled water (37 °C)

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Chitosan: effect of a new chelating agent on the microhardness of root dentin

Cited by 81 publications

References 24 publications

Time-dependent effects of chitosan on dentin structures

Time-dependent effects of chitosan on dentin structures

Adhesive strength of self-adhesive resins to lithium disilicate ceramic and dentin: effect of dentin chelating agents

Avaliação da Resistência de União Entre Resina Composta e Substrato Dentinário Exposto à Quitosana

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