Chitosan from shrimp shells: A renewable sorbent applied to the clean-up step of the QuEChERS method in order to determine multi-residues of veterinary drugs in different types of milk

Abstract

Clean extracts are essential in LC-MS/MS, since the matrix effect can interfere in the analysis. Alternative materials which can be used as sorbents, such as chitosan in the clean-up step, are cheap and green options. In this study, chitosan from shrimp shell waste was evaluated as a sorbent in the QuEChERS method in order to determine multi-residues of veterinary drugs in different types of milk, i. e., fatty matrices. After optimization, the method showed correlation coefficients above 0.99, LOQs ranged between 1 and 50μgkg and recoveries ranged between 62 and 125%, with RSD<20% for all veterinary drugs in all types of milk under study. The clean-up step which employed chitosan proved to be effective, since it reduced both the matrix effect (from values between -40 and -10% to values from -10 to +10%) and the extract turbidity (up to 95%). When the proposed method was applied to different milk samples, residues of albendazole (49μgkg), sulfamethazine (<LOQ) and mebendazole (<LOQ) were found.

Potency of Chitosan and Chitooligochitosan (COS) as Prebiotics for Streptococcus thermophillus and Lactobacillus bulgaricus Probiotics

“…Chitosan is generated from chitin deacetilation that produce some good benefits due to its physicochemistry and biological aspects (Islam et al, 2020) in terms of food industry, pharmacy and environemental agent (Arias et al, 2018). The weakness of this compound is due to the low water solubility with high viscosity (Tanasale et al, 2019).…”

Use of Micellar Liquid Chromatography to Determine Mebendazole in Dairy Products and Breeding Waste from Bovine Animals

“…Thus, there is a need to develop and validate a simple method that is reliable, Several research articles are available on the determination of veterinary drugs and their residues in animal derived food products. Most of the reported analytical methods use high performance liquid chromatography (HPLC) coupled with fixed wavelength ultraviolet [12,13], photo diode array [14] or mass spectrometry [15,16] detector.…”

DETERMINAÇÃO DE AGROTÓXICOS EM AMOSTRAS DE ARROZ EMPREGANDO MÉTODO QuEChERS e GC-MS

“…So to guarantee high production, the extensive usage of pesticides for rice production is a common practice. According to the latest report from PARA (2017-2018) (ANVISA, 2018) 28.5% of the rice samples evaluated presented residues in concentrations equal to or less than the MRL, and among the compounds found the tebuconzale fungicide, from the triazole group, presented the highest number of detections (67%). Despite being considered safe for humans under low consumption conditions, there is concern about the use of this pesticide since its ability to cause malformations in animal cells has been reported, both in vivo and in vitro (Menegola et al, 2013).…”

“…Developed in 2003, the QuEChERS method (Quick, Easy, Cheap, Effective, Rugged and Safe) emerged with the objective of overcoming the practical limitations of the multi-residue extraction methods available at the time (Anastassiades et al, 2003a). Originally designed for the analysis of pesticide residues in fruits and vegetables, the QuEChERS method has been applied to the analysis of different classes of contaminants and residues in different food matrices (Kupski e Badiale-Furlong, 2015;Cabrera et al, 2016;Arias et al, 2018). This method has the advantages of being fast, easy, cheap, economical, robust and safe, being a dynamic procedure, capable of being performed in any laboratory (Prestes et al, 2009) and have been used coupled especially with LC-MS/MS for the detection of different pesticides in rice samples (Cabrera et al, 2016;Liu et al, 2020;Melo et al, 2020).…”