Potency of Chitosan and Chitooligochitosan (COS) as Prebiotics for Streptococcus thermophillus and Lactobacillus bulgaricus Probiotics

Abstract

Lately, chitosan as a result of chitin deacetilation has known as potencial compound as food industry, pharmacy and environmental agents. The weakness of chitosan is the low water solubility and high viscosity. The size reduction by cutting the β-1, 4 glicosidic bond to improve the bioactivity is the alternative solution. This research aims to find out the addition of chitosan and COS for the Streptococcus thermophillus FNCC – 0041 and Lactobacillus bulgaricus FNCC – 0040 probiotics bacteria culture. S. thermophillus and L. bulgaricus were cultured in the MRS Broth media with addition of Commercial Chitosan (CC), Commercial Oligochitosan (COC), Self-production Chitosan (PC) and Self-production Oligochitosan (POC) in different doses (0.05; 0.1; and 0.2 mg.ml-1). Percentage DD on those treatments was similar. The spectra vibration of The FT-IR analysis of PC and POC were fit to the CC and COC. In chitosan treatments, the best growth of S. thermophilus was reached at 0.2 mg/mL PC, while in chitooligochitosan was from 0.20 mg/mL COC treatments, respectively. The addition of different type of chitosan have a significant effect (p<0.05) to the growth of S. thermophillus, but have not resulted the significant effect to the L. bulgaricus (p>0.05) growth. So, therefore the addition of chitosan and COS as prebiotics for the probiotics were in dose dependant manner. Compare to chitosan commercial, our chitosan production have a good potency to be developed.

Long-Term Application of a Synbiotic Chitosan and Acinetobacter KU011TH Mixture on the Growth Performance, Health Status, and Disease Resistance of Hybrid Catfish (Clarias gariepinus × C. macrocephalus) during Winter

“…The current study contributes to the growing research on probiotics, prebiotics, and synbiotics for eco-friendly aquaculture. Specifically, it focused on exploring the potential applications of combining Acinetobacter KU011TH probiotics with chitosan, which has strong prebiotic properties and coating efficiency [ 45 , 46 ], as a synbiotic for use in catfish aquaculture [ 47 , 48 ]. This study is believed to be the first to investigate the supplementation of the genus Acinetobacter with chitosan and its use as a potential synbiotic in aquatic vertebrates under low-temperature and low-alkalinity conditions.…”

Artemia with Synbiotics Enrichment Improves Resistance Against Vibrio parahaemolyticus AHPND of Litopenaeus vannamei Larvae

“…A serial concentration of alginate 0, 400, 600, dan 800 ppm was adjusted by adding 0.006, 0.012, and 0.018 g Alginate and diluted into 20 mL liquid culture of FNCC-004 dan 180 mL sterile sea water. The FNCC-004 and alginate fermentation was done by stirring at 150 rpm at room temperature (Yudiati et al, 2021).…”

“…The enrichment of Artemia nauplli was done by immersion. 250-500 nauplii Artemia was immersed in 50 mL fermented probiotics and alginate for one hour (Yudiati et al, 2021). The enriched Artemia is ready to use as natural feed for L. vannamei PL.…”

“…Alginate, the water extracted from Sargassum sp cell wall is well known as immunostimulant agent (Isnansetyo et al, 2015;Yudiati et al, 2016;Yudiati et al, 2019) and worked synergically as synbiotics with Lactobacillus bulgaricus probiotics (Yudiati et al, 2021). Furthermore, Du et al (2019) denoted that Lactobacillus pentosus is able to increase disease resistance, gut bacterial diversity, and growth performance.…”

“…We have evaluated that sodium alginate has a good potency as synbiotics and had a synergetic mechanism with L. bugaricus probiotics to counteract the combat of three single and combined Vibrio spp. in Artemia as biomodel (Yudiati et al, 2021). The key to this mechanism is the enhance of the immune system and the balance of microbiota.…”