Chitosan Functionalized Magnetic Nanoparticles to Provide Neural Regeneration and Recovery after Experimental Model Induced Peripheral Nerve Injury

Pop, Nadina Liana; Nan, Alexandrina; Urda-Cîmpean, Andrada Elena; Florea, Adrian; Toma, Vlad-Alexandru; Moldovan, Remus; Decea, Nicoleta; Mitrea, Daniela-Rodica; Orăsan, Remus Ioan

doi:10.3390/biom11050676

Cited by 23 publications

(9 citation statements)

References 58 publications

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“…Nerve outreach without surgical intervention and better functional outcome versus without treatment [90] Polycaprolactone/chitosanhydroxyapatite hybrid implants Peripheral Possibility of controlling the diffusion of oxygen and nutrients and invariable mechanical properties for up to 28 days [91] The conduits currently available for nerve regeneration are hollow tubes, which are generally associated with poor recovery and difficulty in nerve extension due to scar formation. For this reason, research has focused on the inclusion of fillers (such as some of the examples in Table 1) and growth factors or the Schwann cells in them to enhance the regeneration process [92,93].…”

Section: Sciaticmentioning

confidence: 99%

Applications of Chitosan in Surgical and Post-Surgical Materials

et al. 2022

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The continuous advances in surgical procedures require continuous research regarding materials with surgical applications. Biopolymers are widely studied since they usually provide a biocompatible, biodegradable, and non-toxic material. Among them, chitosan is a promising material for the development of formulations and devices with surgical applications due to its intrinsic bacteriostatic, fungistatic, hemostatic, and analgesic properties. A wide range of products has been manufactured with this polymer, including scaffolds, sponges, hydrogels, meshes, membranes, sutures, fibers, and nanoparticles. The growing interest of researchers in the use of chitosan-based materials for tissue regeneration is obvious due to extensive research in the application of chitosan for the regeneration of bone, nervous tissue, cartilage, and soft tissues. Chitosan can serve as a substance for the administration of cell-growth promoters, as well as a support for cellular growth. Another interesting application of chitosan is hemostasis control, with remarkable results in studies comparing the use of chitosan-based dressings with traditional cotton gauzes. In addition, chitosan-based or chitosan-coated surgical materials provide the formulation with antimicrobial activity that has been highly appreciated not only in dressings but also for surgical sutures or meshes.

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Section: Sciaticmentioning

confidence: 99%

Applications of Chitosan in Surgical and Post-Surgical Materials

et al. 2022

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“…Moreover, IONPs and IONP-loaded cells allow the delivery of therapeutic biomolecules, such as neurotrophic factors, drugs, proteins, DNA and siRNA by specific NP functionalization [403][404][405][406][407]. Functionalized IONPs and IONP-loaded cells can be effectively enriched at the injury site by external magnet fields to efficiently promote neuronal repair or guide axonal growth [408][409][410][411][412][413][414][415]. Finally, the use of scaffolds made of various nanomaterials can serve as structural support, induce or mimic the formation of an ECM, inhibit glial differentiation, promote neuronal growth and control hemostasis.…”

Section: Pns and Cns Regenerationmentioning

confidence: 99%

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

2021

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In recent years, many promising nanotechnological approaches to biomedical research have been developed in order to increase implementation of regenerative medicine and tissue engineering in clinical practice. In the meantime, the use of nanomaterials for the regeneration of diseased or injured tissues is considered advantageous in most areas of medicine. In particular, for the treatment of cardiovascular, osteochondral and neurological defects, but also for the recovery of functions of other organs such as kidney, liver, pancreas, bladder, urethra and for wound healing, nanomaterials are increasingly being developed that serve as scaffolds, mimic the extracellular matrix and promote adhesion or differentiation of cells. This review focuses on the latest developments in regenerative medicine, in which iron oxide nanoparticles (IONPs) play a crucial role for tissue engineering and cell therapy. IONPs are not only enabling the use of non-invasive observation methods to monitor the therapy, but can also accelerate and enhance regeneration, either thanks to their inherent magnetic properties or by functionalization with bioactive or therapeutic compounds, such as drugs, enzymes and growth factors. In addition, the presence of magnetic fields can direct IONP-labeled cells specifically to the site of action or induce cell differentiation into a specific cell type through mechanotransduction.

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“…[ 1 , 2 ]. Although most peripheral nerve injuries do not endanger human life, partial nerve defects hinder functions of normal surrounding tissue and cause action inconvenience and paresthesia to patients, which adversely affects people’s daily life [ 3 , 4 ]. Therefore, the repair of peripheral nerve injury is urgent and plays a vital role in a patient’s life [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Co-culture of Schwann cells and endothelial cells for synergistically regulating dorsal root ganglion behavior on chitosan-based anisotropic topology for peripheral nerve regeneration

Zheng

Sun

et al. 2022

Burns &Amp; Trauma

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Background Anisotropic topologies are known to regulate cell-oriented growth and induce cell differentiation, which is conducive to accelerating nerve regeneration, while co-culture of endothelial cells (ECs) and Schwann cells (SCs) can significantly promote the axon growth of dorsal root ganglion (DRG). However, the synergistic regulation of EC and SC co-culture of DRG behavior on anisotropic topologies is still rarely reported. The study aims to investigate the effect of anisotropic topology co-cultured with Schwann cells and endothelial cells on dorsal root ganglion behavior for promoting peripheral nerve regeneration. Methods Chitosan/artemisia sphaerocephala (CS/AS) scaffolds with anisotropic topology were first prepared using micro-molding technology, and then the surface was modified with dopamine to facilitate cell adhesion and growth. The physical and chemical properties of the scaffolds were characterized through morphology, wettability, surface roughness and component variation. SCs and ECs were co-cultured with DRG cells on anisotropic topology scaffolds to evaluate the axon growth behavior. Results Dopamine-modified topological CS/AS scaffolds had good hydrophilicity and provided an appropriate environment for cell growth. Cellular immunofluorescence showed that in contrast to DRG growth alone, co-culture of SCs and ECs could not only promote the growth of DRG axons, but also offered a stronger guidance for orientation growth of neurons, which could effectively prevent axons from tangling and knotting, and thus may significantly inhibit neurofibroma formation. Moreover, the co-culture of SCs and ECs could promote the release of nerve growth factor and vascular endothelial growth factor, and up-regulate genes relevant to cell proliferation, myelination and skeletal development via the PI3K-Akt, MAPK and cytokine and receptor chemokine pathways. Conclusions The co-culture of SCs and ECs significantly improved the growth behavior of DRG on anisotropic topological scaffolds, which may provide an important basis for the development of nerve grafts in peripheral nerve regeneration.

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Chitosan Functionalized Magnetic Nanoparticles to Provide Neural Regeneration and Recovery after Experimental Model Induced Peripheral Nerve Injury

Cited by 23 publications

References 58 publications

Applications of Chitosan in Surgical and Post-Surgical Materials

Applications of Chitosan in Surgical and Post-Surgical Materials

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

Co-culture of Schwann cells and endothelial cells for synergistically regulating dorsal root ganglion behavior on chitosan-based anisotropic topology for peripheral nerve regeneration

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