Chitosan-Gentamicin Conjugate Hydrogel Promoting Skin Scald Repair

Abstract

Our earlier research indicated that chitosan-gentamicin conjugate (CS-GT) possesses superior antimicrobial activity and good water solubility. To develop CS-GT-based scald dressings, the antibacterial properties of CS-GT were further studied, and the biosafety of CS-GT and the healing mechanism of CS-GT hydrogel was systematically explored in this article. It was found that cell viability shows a declined inclination with the prolonged culture time and the increased concentration of CS-GT. After three day’s culture, the cell viability could still remain at 79.72% when CS-GT concentration was as high as 1000 μg/mL. On the other hand, the hemolysis rate of CS-GT was lower than 5% when its concentration is 800 μg/mL. Therefore CS-GT has good cytocompatibility and hemocompatibility. A wound-healing experiment has shown that the skin healing rate of CS-GT hydrogel was the highest at 99.61%, followed by the positive control (wet burn ointment) 94.98%, GT hydrogel 87.50%, and matrix 77.39%. The blank control group, however, possessed the lowest healing rate of 75.45%. Further analysis indicated that CS-GT hydrogel could promote the synthesis of total protein (TP) in skin granulation tissue, resulting in the enhanced hydroxyproline (HYP) content, which facilitated collagen fibrogenesis, reduced cytokine expression in an inflammatory response, and, ultimately, accelerated wound healing. To sum up, CS-GT hydrogel is a promising scald dressing.

Chitosan and Cellulose-Based Hydrogels for Wound Management

“…On day 14 and 21, the wound healing rate was 89.18% and 99.61%, respectively, in vivo on the animal model treated with the hydrogel. On day 14, complete epidermal regeneration was visible [ 106 ]. Cifuentes et al reported the efficacy of chitosan hydrogels functionalized with either bemiparin or unfractionated heparin for diabetic wound healing.…”

Effects of Chitosan–Gentamicin Conjugate Supplement on Non-Specific Immunity, Aquaculture Water, Intestinal Histology and Microbiota of Pacific White Shrimp (Litopenaeus vannamei)

“…Our previous research also found that CS-GT-based hydrogels, with antimicrobial activity, cytocompatibility, and hemocompatibility, can accelerate the wound healing of New Zealand rabbits [ 27 ]. Particularly, CS-GT can inhibit pathogenic Escherichia coli , Pseudomonas aeruginosa , and Staphylococcus aureus effectively [ 26 ].…”

Hollow polydopamine nanoparticles loading with peptide RL-QN15: a new pro-regenerative therapeutic agent for skin wounds

“…Typically, RL-QN15 was dissolved in PBS solution and the mixed solution's relative concentration was determined via the absorbance at 220 nm determined by a spectrophotometry (NanoDrop One, Thermo, USA). PBS solution containing HPDA (0.5 mg) and RL-QN15 (100 nmol) with a volume of 5 mL was stirred at 4 °C for 24 h to form a nanocomposite solution, then centrifuged (10 000g, 5 min, 4 ℃), and the resulting supernatant was taken at various time-points (2,4,6,8,10,12,16,20, and 24 h) to determine the relative concentration of the unloaded peptide RL-QN15. The loading efficiency of HPDA nanoparticles against RL-QN15 was calculated according to the equation: Loading efficiency (%) = [C (0) − C (2,4,6,8,10,12,16,20,24)] / C (0) × 100, where C (0) represented the initial concentration, and C (2, 4, 6, 8, 10, 12, 16, 20, and 24) represented the concentrations at 2, 4, 6, 8, 10, 12, 16, 20, and 24 h, respectively [19,30].…”

“…As the largest organ of the human body, skin participates in many important physiological functions, such as sweating, fever, and pain perception [ 1 , 2 ]. Skin is also an important barrier between the internal body and external environment, protecting internal organs and tissues from skin rupture caused by physical, mechanical, and chemical factors and infections caused by pathogenic microorganisms [ 3 ].…”