Chitosan, hyaluronan and chondroitin sulfate in tissue engineering for cartilage regeneration: A review

Müzzarelli, Riccardo A.A.; Greco, Francesco; Busilacchi, Alberto; Sollazzo, Vincenzo; Gigante, Antonio

doi:10.1016/j.carbpol.2012.04.057

Cited by 401 publications

(202 citation statements)

References 175 publications

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“…In a previous study, administration of CS solution into the murine knee joint led to a significant increase in the density of newly formed chondrocytes, which indicated that it was able to facilitate the healing of the cartilage (19). Furthermore, it was revealed that the percentage of viable cells in the CS-CrmA group was significantly higher than that in the IL-1β group, a finding that was in accordance with that of a recent study by the present authors (7).…”

Section: Discussionsupporting

confidence: 92%

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Protective effect of controlled release of cytokine response modifier A from chitosan microspheres on rat chondrocytes from interleukin-1β induced inflammation and apoptosis

Zhou

Shi

Qiu

2017

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to investigate the protective effect of cytokine response modifier A (CrmA) released from chitosan (CS) microspheres in a controlled manner on interleukin (IL)-1β-induced inflammation and apoptosis in chondrocytes. The CrmA release kinetics were characterized by an initial burst release, which was reduced to a linear release over 8 days. Furthermore, chondrocytes were isolated from 1-week-old Sprague Dawley rats. The cell culture was established by stimulation with 10 ng/ml IL-1β and subsequent incubation with CS-CrmA microspheres. Following stimulation with IL-1β, the viability of chondrocytes was decreased. However, the cell viability was attenuated by CS-CrmA microspheres as revealed by a cell counting kit-8 assay. CS-CrmA microspheres significantly inhibited IL-1β-induced inflammation in chondrocytes by attenuating increases in the gene expression levels of inducible nitric oxide synthase and cyclooxygenase-2, as well as the concentrations of nitric oxide and prostaglandin E2. CS-CrmA microspheres significantly decreased the number of apoptotic chondrocytes induced by IL-1β as indicated by a terminal deoxyribonucleotide transferase deoxyuridine triphosphate nick-end labeling assay. In addition, CS-CrmA microspheres blocked IL-1β-induced chondrocyte apoptosis by increasing B-cell lymphoma 2 (Bcl-2) and decreasing Bcl-2-associated X protein, caspase-3 and poly adenosine diphosphate-ribose polymerase expression at the mRNA and protein levels, as indicated by reverse-transcription quantitative polymerase chain reaction and western blot analysis, respectively. The results of the present study revealed that CS-CrmA microspheres, as a controlled release system of CrmA, may protect rat chondrocytes from IL-1β-induced inflammation and apoptosis via regulating inflammatory and apoptosis-associated genes.

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Section: Discussionsupporting

confidence: 92%

“…It has been widely used in drug, DNA delivery and tissue engineering due to its non-toxicity, biocompatibility and biodegradability (16)(17)(18). Furthermore, it promotes attachment, proliferation and viability of mesenchymal stem cells, and due to these features, CS and its derivatives are considered as promising biomaterials (19).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of controlled release of cytokine response modifier A from chitosan microspheres on rat chondrocytes from interleukin-1β induced inflammation and apoptosis

Zhou

Shi

Qiu

2017

Experimental and Therapeutic Medicine

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“…10 Impact of age group on cell viability after supplementation with UCM on the 6th day (age groups in years, A: 20-30; B: 30-40; C: 40-50; D: 50-60) chondrocytes (Jerosch 2011) and stimulate anabolic processes leading to neocartilage formation (Levett et al 2014). Muzzarelli et al (2012) reported that CS plays an important role in cartilage regeneration by creating a chondrocyte inducing environment which paves way for cell growth and ECM deposition. CS can bind with core proteins to produce aggregan that acts as a shock absorber inside cartilage (Little et al 2014).…”

Section: Discussionmentioning

confidence: 99%

A combination of biomolecules enhances expression of E-cadherin and peroxisome proliferator-activated receptor gene leading to increased cell proliferation in primary human meniscal cells: an in vitro study

et al. 2015

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The present study investigates the impact of biomolecules (biotin, glucose, chondroitin sulphate, proline) as supplement, (individual and in combination) on primary human meniscus cell proliferation. Primary human meniscus cells isolated from patients undergoing meniscectomy were maintained in Dulbecco's Modified Eagle's Medium (DMEM). The isolated cells were treated with above mentioned biomolecules as individual (0-100 lg/ml) and in combinations, as a supplement to DMEM. Based on the individual biomolecule study, a unique combination of biomolecules (UCM) was finalized using one way ANOVA analysis. With the addition of UCM as supplement to DMEM, meniscal cells reached 100 % confluency within 4 days in 60 mm culture plate; whereas the cells in medium devoid of UCM, required 36 days for reaching confluency. The impact of UCM on cell viability, doubling time, histology, gene expression, biomarkers expression, extra cellular matrix synthesis, meniscus cell proliferation with respect to passages and donor's age were investigated. The gene expression studies for E-cadherin and peroxisome proliferator-activated receptor (PPARD) using RT-qPCR and immunohistochemical analysis for Ki67, CD34 and Vimentin confirmed that UCM has significant impact on cell proliferation. The extracellular collagen and glycosaminoglycan secretion in cells supplemented with UCM were found to increase by 31 and 37 fold respectively, when compared to control on the 4th day. The cell doubling time was reduced significantly when supplemented with UCM. The addition of UCM showed positive influence on different passages and age groups. Hence, this optimized UCM can be used as an effective supplement for meniscal tissue engineering.

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“…Like the native cartilage, this polymer contains glycosaminoglycan (GAG) and hyaluronic acid, presents properties such as biocompatibility, biodegradability and non-toxicity and in the last years it was very used in the cartilage tissue engineering field. The main disadvantage of this polymer refers to the lack of gelling properties, leading to the possibility that it will flow out of the joint when applied, forming cartilage-like tissue ectopically [19,20,28].…”

Section: Biomaterials In Cartilage Tissue Engineeringmentioning

confidence: 99%

Biomaterials for Cartilage Tissue Engineering

Grigore¹

2017

J Tissue Sci Eng

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One of the most challenging issues of musculoskeletal medicine is represented by injuries of the articular cartilage due to the poor regenerative properties of this tissue. A consequence of these injuries is represented by osteoarthritis. Osteoarthritis is the most common chronic condition of the joints, caused because of the progressive wear and tear on articular cartilage. A solution to prevent progressive joint degeneration in osteoarthritis is represented by a surgical intervention which offers the advantage of the success of total joint replacement, but also offers several disadvantages such as such as slower remodeling, immune reaction and disease transmission. In the last years, the researchers have found a solution to avoid surgical intervention by using biomaterials. This study aims to provide an updated survey of the major progress in the flied of biomaterials for cartilage tissue engineering, including biomaterials (natural, synthetic or composites), their advantages or disadvantages and the main seeding cell sources. Also, this review focuses on the progress made in the field of biomaterials for cartilage tissue repair and/or regeneration over the last years.

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Chitosan, hyaluronan and chondroitin sulfate in tissue engineering for cartilage regeneration: A review

Cited by 401 publications

References 175 publications

Protective effect of controlled release of cytokine response modifier A from chitosan microspheres on rat chondrocytes from interleukin-1β induced inflammation and apoptosis

Protective effect of controlled release of cytokine response modifier A from chitosan microspheres on rat chondrocytes from interleukin-1β induced inflammation and apoptosis

A combination of biomolecules enhances expression of E-cadherin and peroxisome proliferator-activated receptor gene leading to increased cell proliferation in primary human meniscal cells: an in vitro study

Biomaterials for Cartilage Tissue Engineering

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