Chitosan/hyaluronan/edaravone membranes for anti-inflammatory wound dressing: In vitro and in vivo evaluation studies

Tamer, Tamer M.; Valachová, Katarína; Hassan, Mohamed A.; Omer, Ahmed M.; El-Shafeey, Muhammad; Eldin, M.S. Mohy; Šoltés, Ladislav

doi:10.1016/j.msec.2018.04.053

Cited by 111 publications

(49 citation statements)

References 52 publications

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“…The property of nanofibrous biodegradation is beneficial for skin regeneration as it can support and regulate skin regeneration [20]. Gelatin absorbed an amount of water and made the nanofibrous more hydrophilic and sensitive to degradation, while more chitosan exhibited slower degradation, which may inhibit wound healing [26]. So the crosslinked electrospun gelatin/chitosan (7:3) nanofibrous with appropriate biodegradation may be more suitable for skin regeneration.…”

Section: Discussionmentioning

confidence: 99%

Construction of Sandwich-like cell sheets with natural electrospun gelatin/chitosan nanofibrous delivered plasmid VEGF for accelerated wound healing

Zhu

Liao

Zhang

et al. 2020

Preprint

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Background Development of natural biodegradable electrospun nanofibrous with appropriate physical properties and biocompatibility is highly desirable to support multi-layer cell sheets construction for wound healing. Results We developed a series of electrospun gelatin/chitosan nanofibrous with different gelatin/chitosan ratios and controlled pore sizes, and impregnated plasmid VEGF into membrane, which as supporting membrane to construct sandwich-like adipose-derived stem cells (ADSCs) cell sheets with a simple and effective technique for accelerated wound healing. We found that the physical properties of the electrospun nanofibrous including water retention, stiffness, strength, elasticity and degradation could be tailored by changing the proportion of gelatin/chitosan. We further observed that the optimized electrospun nanofibrous with the optimal ratio of gelatin to chitosan (7:3) which were soft and elastic could most effectively support cell adhesion, proliferation and migration into the whole nanofibrous membranes. Nanofibrous delivered plasmid VEGF facilitating multi-layer ADSC cell sheets formation and promoting regeneration of cutaneous tissues within two weeks. Conclusions Such natural biodegradable and biocompatible electrospun gelatin/chitosan nanofibrous with plasmid have the potential to become fully cellularized and support sandwich-like ADSC cell sheets formation, which will make it suitable for widespread applications such as skin substitute or wound dressing.

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Section: Discussionmentioning

confidence: 99%

Construction of Sandwich-like cell sheets with natural electrospun gelatin/chitosan nanofibrous delivered plasmid VEGF for accelerated wound healing

Zhu

Liao

Zhang

et al. 2020

Preprint

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“…[18,30] 0.1 g of QCS dissolved in 100 ml milli-Q water was titrated against 0.017 M AgNO 3 and its conductivity was measured using a digital conductivity meter (Cole-Parmer OAKTON PC 2700, Singapore). The volume of AgNO 3 solution corresponding to the lowest conductivity of the QCS solution was noted and the degree of substitution was measured by the equation (4)…”

Section: Estimation Of Degree Of Quaternizationmentioning

confidence: 99%

“…Natural biopolymers acquired superior significance in the recent past and have been extensively studied in the present decade towards the development of efficient antimicrobial, [1,2] antioxidant, [1] drug delivery, [3] wound healing systems, [4] and so on. due to their inherent biocompatible and biofunctional properties.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Polyelectrolytic chitosan derivatives conjugated natural rubber latex films with minimized bacterial adhesion

Arakkal

Aazem

Honey

et al. 2020

J of Applied Polymer Sci

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Water soluble polyelectrolytic derivatives of chitosan (CDs) with carboxyl (COO −) and quaternary ammonium (NH 4 +) groups were synthesized and characterized by NMR, FT-IR, TGA and zeta potential analyses; while degree of substitution was determined by potentiometric titrations. Both CDs in the as-synthesized form exhibited antibacterial zones against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The CD conjugated vulcanized natural rubber latex (NRL) films were investigated for understanding the level of bacterial adhesion and bactericidal properties as a function of the polyelectrolytic groups present. NRL Films modified at ambient conditions were found effective against Pseudomonas aeruginosa (P. aeruginosa). The biofilm assay results demonstrate that CD conjugated samples exhibit significantly reduced adhesion (63.8%) against P. aeruginosa. Further, alterations observed in cellular morphology of attached bacteria indicate that the modified surface of films is bacteriocidal while presence of significantly higher level of extra cellular polymer substances (EPS) were detected on control NRL films.

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“…Luego se dispuso la solución en cápsulas de Petri hasta cubrir el fondo de la misma y se dejó secando a temperatura ambiente durante 5 días. Una vez seca la película, se despegaron de las cápsulas y se neutralizaron sumergiéndolas en una solución de NaOH 1 M por 30 s. Finalmente se lavaron sumergiéndolas en agua nanopura durante 2 min [38].…”

Section: Preparación De Membranasunclassified

Síntesis y caracterización de un novedoso biomaterial a base de quitosano modificado con aminoácidos

et al. 2019

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RESUMEN Se realizó la modificación química de quitosano (CH) proveniente de conchas de camarón (Litopenaeus vannamei) con aminoácidos: L-leucina (Leu) y L-tirosina (Tyr), con el fin de variar algunas de sus propiedades físicas y químicas, y ampliar su uso en la fabricación de películas y andamios, potenciando las aplicaciones del quitosano en áreas biomédicas y farmacológicas. Los derivados (CH-Leu y CH-Tyr) fueron caracterizados mediante estudios de espectroscopia infrarrojo (ATR-FTIR) para verificar la estructura de los productos modificados y las propiedades térmicas fueron analizadas mediante termogravimetría (TGA). Se realizaron ensayos de solubilidad, donde se observó que los derivados obtenidos son solubles en disoluciones acuosas a valores de pH neutro y alcalino, a diferencia del quitosano que solo es soluble bajo condiciones de pH ácido. La citotoxicidad de los derivados fue analizada mediante ensayos de hemocompatibilidad con eritrocitos humanos. Posteriormente se realizaron membranas a partir de los derivados de CH-Leu y CH-Tyr, y se determinó el ángulo de contacto. El proceso de biodegradabilidad en membranas se estudió a través de procesos de hidrólisis en H2O y buffer fosfato salino (PBS), pH 7,4 a 37ºC durante intervalos de tiempo de 24, 72 y 168 h. Los andamios fueron preparados usando la técnica de electrospinning al preparar una mezcla entre poli(vinil alcohol) (PVA) y los derivados de CH (dada la solubilidad mostrada en agua), y la morfología de las fibras fue estudiada mediante microscopía electrónica de barrido (MEB). En general, los resultados obtenidos por FTIR, TGA y MEB sirvieron como herramientas para verificar la modificación, y estos derivados permiten ampliar las propiedades físicas y químicas del quitosano puro, principalmente en cuanto a su solubilidad, permitiendo que este novedoso biomaterial sea empleado en múltiples aplicaciones, resaltando que pueden ser mezclados con fases poliméricas sintéticas lo cual abre ventanas para el uso de este interesante biopolímero.

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Chitosan/hyaluronan/edaravone membranes for anti-inflammatory wound dressing: In vitro and in vivo evaluation studies

Cited by 111 publications

References 52 publications

Construction of Sandwich-like cell sheets with natural electrospun gelatin/chitosan nanofibrous delivered plasmid VEGF for accelerated wound healing

Construction of Sandwich-like cell sheets with natural electrospun gelatin/chitosan nanofibrous delivered plasmid VEGF for accelerated wound healing

Antibacterial Polyelectrolytic chitosan derivatives conjugated natural rubber latex films with minimized bacterial adhesion

Síntesis y caracterización de un novedoso biomaterial a base de quitosano modificado con aminoácidos

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