Chitosan kills bacteria through cell membrane damage

Liu, Hui; Du, Yumin; Wang, Xiaohui; Sun, Liping

doi:10.1016/j.ijfoodmicro.2004.01.022

Cited by 746 publications

(379 citation statements)

References 23 publications

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“…In general, chitosan has been reported to show strong inhibition towards E. coli, but differs with several factors such as molecular weight. Previous study on the bactericidal activity of chitosan against E. coli has shown that chitosan caused lysis and cytoplasmic membrane disruption in E. coli observed via ultra-structural analysis using TEM 35 .The similar study also suggested that the electrostatic interaction between -NH 3 + groups of chitosan and the negatively charged phospholipids component (carbonyl and phosphoryl groups) of the cell membrane eventually brought about damage to the bacterial cell membrane 36 . Other study has also reported that the release of protonate glucosamine (due to the protonation of amine group) from the chitosan film could contribute to the inhibition of bacterial growth 37 .…”

Section: Antibacterial Studymentioning

confidence: 69%

Composite Film of Chitosan Loaded Norfloxacin With Improved Flexibility and Antibacterial Activity for Wound Dressing Application

Sebri¹,

Amin²

2017

Orient. J. Chem

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This research develops chitosan film incorporated norfloxacin (CH-NOR) designed to combat the infection of bacteria around the wound area. Inclusion of norfloxacin increased the strain-atbreak or flexibility of the film. Swelling behavior and water vapor transmission rates (WVTRs) values are improved and optimum for CH-NOR1.0 film at 524 ± 65 % and 1916 ± 78 g m -2 d -1 , respectively. In-vitro cell studies show that the present of norfloxacin in CH film gradually decreased the number of human dermal fibroblast cell (CRL2522, ATCC) after incubated for 72 hours. In-contrast, antibacterial activities of CH-NOR films exhibit strong inhibition zones against Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative (Escherichia coli and Klebsiella pneumoniae) bacteria.

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Section: Antibacterial Studymentioning

confidence: 69%

Composite Film of Chitosan Loaded Norfloxacin With Improved Flexibility and Antibacterial Activity for Wound Dressing Application

Sebri¹,

Amin²

2017

Orient. J. Chem

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“…The significant morphological changes in both chitosan and chitosanmetal complexes indicate the formation of complexes. and Ch-Co) [13,14,15]. The specific peaks of chitosan and Ch-Fe complex were observed at diffraction angles of 19º and 22º, respectively, indicating that both chitosan and Ch-Fe complex as were amorphous.…”

Section: Resultsmentioning

confidence: 97%

Morphological, Thermal and Electrical Studies on Chitosan Heavy Metal Complexes

Husain

El-Hady

Sayed

et al. 2010

Al-Azhar Bulletin of Science

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The chitosan was prepared and mixed with some metal salts (FeCl3, Co(CH3COO)2 and NiCl2) with different concentrations to form chitosan-metal complexes. The characterizations of these complexes were carried out by FTIR and their morphological studies were carried out by scanning electron microscope (SEM) and wide angle X-ray diffraction apparatus. The thermo gravimetric analysis (TGA) and differential scanning calorimetric (DSC) also were carried out. The metal ions which strongly complexed to the amino groups of chitosan like Fe + ++ showed a smooth surface product, amorphous phase and thermally more stable than other complexes. The chitosan-metal complexes have a higher electrical conductivity than chitosan compounds at room temperature.

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“…Furthermore, coadministration of 0.1 to 0.5 mg of chitosan with V. vulnificus infections in mice significantly increased survival and decreased dissemination in mice (29). Chitosan contains positively charged molecules that bind to negatively charged structures on cell surfaces and subsequently induce the leakage of intracellular material from bacterial cells (26,44,47). Exposure to water-soluble fractions of chitin has been shown to induce competence in V. cholerae and V. vulnificus for uptake of DNA and is used in molecular biology for transformation experiments (48,49).…”

Section: Discussionmentioning

confidence: 99%

“…Chitosan-mediated systems can significantly improve the bioavailability of drug delivery and are categorized as nanoparticle, microparticle, or macrodelivery systems (17,24,25). Furthermore, the antimicrobial activity of chitosan against both Gram-positive and Gram-negative pathogens as well as against food spoilage bacteria has been well demonstrated (26,27).…”

mentioning

confidence: 99%

Application of Chitosan Microparticles for Reduction of Vibrio Species in Seawater and Live Oysters (Crassostrea virginica)

Fang

Wolmarans

Kang

et al. 2015

Appl Environ Microbiol

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bHuman Vibrio infections associated with consumption of raw shellfish greatly impact the seafood industry. Vibrio cholerae-related disease is occasionally attributed to seafood, but V. vulnificus and V. parahaemolyticus are the primary targets of postharvest processing (PHP) efforts in the United States, as they pose the greatest threat to the industry. Most successful PHP treatments for Vibrio reduction also kill the molluscs and are not suitable for the lucrative half-shell market, while nonlethal practices are generally less effective. Therefore, novel intervention strategies for Vibrio reduction are needed for live oyster products. Chitosan is a bioactive derivative of chitin that is generally recognized as safe as a food additive by the FDA, and chitosan microparticles (CMs) were investigated in the present study as a potential PHP treatment for live oyster applications. Treatment of broth cultures with 0.5% (wt/vol) CMs resulted in growth cessation of V. cholerae, V. vulnificus, and V. parahaemolyticus, reducing culturable levels to nondetectable amounts after 3 h in three independent experiments. Furthermore, a similar treatment in artificial seawater at 4, 25, and 37°C reduced V. vulnificus levels by ca. 7 log CFU/ml after 24 h of exposure, but 48 h of exposure and elevated temperature were required to achieve similar results for V. parahaemolyticus and V. cholerae. Live oysters that either were artificially inoculated or contained natural populations of V. vulnificus and V. parahaemolyticus showed significant and consistent reductions following CM treatment (5%) compared to the amounts in the untreated controls. Thus, the results strongly support the promising potential for the application of CMs as a PHP treatment to reduce Vibrio spp. in intact live oysters.V ibrio species cause a significant proportion of human infections associated with consumption of raw or undercooked shellfish, particularly raw oysters (1). The primary pathogens in the United States are Vibrio vulnificus and V. parahaemolyticus; however, in 2011 the first U.S. outbreak of cholera in recent history was attributed to the consumption of oysters contaminated with V. cholerae O75 (2). Unlike other foodborne pathogens associated with seafood, Vibrio spp. occur naturally in estuarine environments, and their abundance is seasonal (3, 4). During warmer months (when the water temperature is Ͼ20°C), nearly all oysters harvested from U.S. Gulf Coast waters harbor V. vulnificus and/or V. parahaemolyticus, with the highest densities periodically exceeding 10 4 most probable number (MPN)/g (5). Despite extensive efforts employing hazard analysis and critical control point approaches and improved sanitation by the seafood industry, the incidence of seafood-associated cases continues to escalate, particularly during summer months, perhaps as a consequence of increasing global water temperatures (6). Annual reports of the incidence of Vibrio-related disease per 100,000 population increased from 0.09 to 0.28 in the Cholera and Other Vibrio Illness ...

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Chitosan kills bacteria through cell membrane damage

Cited by 746 publications

References 23 publications

Composite Film of Chitosan Loaded Norfloxacin With Improved Flexibility and Antibacterial Activity for Wound Dressing Application

Composite Film of Chitosan Loaded Norfloxacin With Improved Flexibility and Antibacterial Activity for Wound Dressing Application

Morphological, Thermal and Electrical Studies on Chitosan Heavy Metal Complexes

Application of Chitosan Microparticles for Reduction of Vibrio Species in Seawater and Live Oysters (Crassostrea virginica)

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