Chitosan/ Mimosa tenuiflora films as potential cellular patch for skin regeneration

Valencia-Gómez, Laura Elizabeth; Martel-Estrada, Santos-Adriana; Vargas-Requena, Claudia; Rivera‐Armenta, José Luis; Alba-Baena, Noé; Rodríguez-Gónzalez, C.A.; Olivas–Armendáriz, I.

doi:10.1016/j.ijbiomac.2016.09.083

Cited by 30 publications

(19 citation statements)

References 36 publications

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“…Mt ethanolic extract has been used as an antibacterial agent for gram negatives, gram positives, and yeast [9]. Also, as antiprotozoal (using flavonoids of leaves and flowers of Mt) [10] and on skin regeneration [6]. In addition, it has the potential to heal severe skin ulcers, whose properties were attributed to molecules and polyphenols of Mt bark [11].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis

et al. 2019

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Synthesis of gold nanoparticles (AuNPs) with plant extracts has gained great interest in the field of biomedicine due to its wide variety of health applications. In the present work, AuNPs were synthesized with Mimosa tenuiflora (Mt) bark extract at different metallic precursor concentrations. Mt extract was obtained by mixing the tree bark in ethanol-water. The antioxidant capacity of extract was evaluated using 2,2-diphenyl-1-picrylhydrazyl and total polyphenol assay. AuNPs were characterized by transmission electron microscopy, X-ray diffraction, UV-Vis and Fourier transform infrared spectroscopy, and X-ray photoelectron spectrometry for functional group determination onto their surface. AuMt (colloids formed by AuNPs and molecules of Mt) exhibit multiple shapes with sizes between 20 and 200 nm. AuMt were tested on methylene blue degradation in homogeneous catalysis adding sodium borohydride. The smallest NPs (AuMt1) have a degradation coefficient of 0.008/s and reach 50% degradation in 190s. Cell viability and cytotoxicity were evaluated in human umbilical vein endothelial cells (HUVEC), and a moderate cytotoxic effect at 24 and 48 h was found. However, toxicity does not behave in a dose-dependent manner. Cellular internalization of AuMt on HUVEC cells was analyzed by confocal laser scanning microscopy. For AuMt1, it can be observed that the material is dispersed into the cytoplasm, while in AuMt2, the material is concentrated in the nuclear periphery.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis

et al. 2019

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“…Moreover, no stress fibers are observed and the skin is stimulated to reconstruction under lupeol treatment [14]. The latest studies relating to the cosmetic or pharmaceutical application of lupeol-rich extract are focused on treating and preventing a connective tissue degeneration [15,16].…”

Section: Introductionmentioning

confidence: 99%

New lupeol esters as active substances in the treatment of skin damage

et al. 2019

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The purpose of the research was to obtain new derivatives of natural triterpene lupeol and to evaluate their potential as active substances in the treatment of skin damage. Four new lupeol esters (propionate, succinate, isonicotinate and acetylsalicylate) and lupeol acetate were obtained using an eco-friendly synthesis method. In the esterification process, the commonly used hazardous reagents in this type of synthesis were replaced by safe ones. This unconventional, eco-friendly, method is particularly important because the compounds obtained are potentially active substances in skin care formulations. Even trace amounts of hazardous reagents can have a toxic effect on damaged or irritated tissues. The molecular structure of the esters were confirmed by 1 H NMR, 13 C NMR and IR spectroscopy methods. Their crystal structures were determined using XRD method. To complete the analysis of their characteristics, physicochemical properties (melting point, lipophilicity, water solubility) and biological activity of the lupeol derivatives were studied. Results of an irritant potential test, carried out on Reconstructed Human Epidermis (RHE), confirmed that the synthesized lupeol derivatives are not cytotoxic and they stimulate a process of human cell proliferation. The safety of use for tested compounds was determined in a cell viability test (cytotoxicity detection kit based on the measurement of lactate dehydrogenase activity) for keratinocytes and fibroblasts. The results obtained showed that the modification of lupeol structure improve its bioavailability and activity. All of the esters penetrate the stratum corneum and the upper layers of the dermis better than the maternal lupeol. Lupeol isonicotinate, acetate and propionate were the most effective compounds in a stimulation of the human skin cell proliferation process. This combination resulted in an increase in the concentration of cells of more than 30% in comparison to control samples. The results indicate that the chemical modification of lupeol allows to obtain promising active substances for treatment of skin damage, including thermal, chemical and radiation burns.

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“…10 In recent years, the study of extracts and powder of this plant for medical applications and in tissue engineering has increased. [10][11][12][13][14] According to the properties described above, it is reasonable to expect that a blend of O-carboxymethyl chitosan (OCMC) and Mimosa tenuiflora could have potential use in tissue engineering. So, in order to evaluate the potential application of the composite as a wound dressing, in this work, the synthesis, morphology, mechanical properties, contact angle, swelling test, enzymatic degradation, antimicrobial properties, in vitro biocompatibility, and wound healing of the film were evaluated.…”

Section: Introductionmentioning

confidence: 99%

Characterization and evaluation of a novel O-carboxymethyl chitosan films with Mimosa tenuiflora extract for skin regeneration and wound healing

Valencia-Gómez

Martel-Estrada

Vargas-Requena

et al. 2019

Journal of Bioactive and Compatible Polymers

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In this study, films of O-carboxymethyl chitosan with Mimosa tenuiflora extract were manufactured, characterized, and evaluated. In this work, both the synthesis of O-carboxymethyl chitosan and the extraction of the active ingredient of Mimosa tenuiflora extract from the cortex are described. First, the extract of Mimosa tenuiflora in water was obtained by precipitation with ethanol, filtering, and concentrating. Subsequently, a study was conducted of scratch wound healing to determine the optimal concentration of extract to be used in the manufacture of films. The produced O-carboxymethyl chitosan films and the Mimosa tenuiflora extract were mixed, and their chemical composition, tensile properties, and wettability were characterized by Fourier-transform infrared spectroscopy, mechanical tests, and contact angle measurement. The antimicrobial properties of the films were tested by turbidimetry using two types of bacteria. In addition, a study of the enzymatic degradation of the films with the enzyme lysozyme was performed. Finally, in vitro studies to assess the biocompatibility and cytotoxicity of films with fibroblastic cells were carried out as well as the kinetic analysis of healing in mice. It was found that the addition of Mimosa tenuiflora extract in the polymer matrix of the films made with O-carboxymethyl chitosan improves the proliferation of fibroblast and accelerates wound healing, thus providing a novel biomaterial for skin regeneration.

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Chitosan/ Mimosa tenuiflora films as potential cellular patch for skin regeneration

Cited by 30 publications

References 36 publications

Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis

Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis

New lupeol esters as active substances in the treatment of skin damage

Characterization and evaluation of a novel O-carboxymethyl chitosan films with Mimosa tenuiflora extract for skin regeneration and wound healing

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