Abstract:In this study, films of O-carboxymethyl chitosan with Mimosa tenuiflora extract were manufactured, characterized, and evaluated. In this work, both the synthesis of O-carboxymethyl chitosan and the extraction of the active ingredient of Mimosa tenuiflora extract from the cortex are described. First, the extract of Mimosa tenuiflora in water was obtained by precipitation with ethanol, filtering, and concentrating. Subsequently, a study was conducted of scratch wound healing to determine the optimal concentratio… Show more
“…Considering the interaction of the lysozyme and the polymer matrix, since O-CMQ is a polysaccharide, it is degraded by enzymatic hydrolysis. 13 In other studies, in scaffolds made with gelatin, chitosan, and hydroxyapatite, it was shown that the degradation increases with the incorporation of gelatin but decreases when the hydroxyapatite is added to the biomaterial, 22 a similar trend was observe in the composite films with Ag-Cu/HA, compared to the OCMC and gelatin control film. The changes in pH seen during the biodegradation period of the material were monitored during the 72h exposure of 1 X PBS with lysozyme (Figure 5(b)).…”
Section: Enzymatic Degradationmentioning
confidence: 73%
“…Considering the interaction of the lysozyme and the polymer matrix, since O-CMQ is a polysaccharide, it is degraded by enzymatic hydrolysis. 13 Figure 5.Degradation enzymatic results: (a) the weight loss percentage of de films during enzymatic degradation on 1, 2, and 3 days, data represent mean ± S.D. ( n = 5), (b) pH variation of the PBA solution during enzymatic degradation on 1, 2 and 3 days, (c) Observation of the morphology and chemical composition of the surface by SEM-EDS of the C9 sample: (c1) day 1, (c2) day 2 and, (c3) day 3.Figure 6.(a) Antibacterial properties films result on Gram – and Gram + bacteria at 24 h, (b) Cell viability percentage of fibroblasts seeded on the films at 24 and 72 h. Data represents mean ± S.D.…”
Wound dressing composite films of O-carboxymethyl chitosan (OCMC) and gelatin were prepared and mixed with hydroxyapatite (HA) composited with Silver (Ag) and Copper (Cu) at different concentrations. The chemical, thermal, morphological, and biological properties of the composite films were studied. The analysis by FTIR confirmed the presence of interactions between gelatin and OCMC, and at the same time, the polymer matrix interactions with Ag-Cu/HA complex. The inclusion of nanoparticle to the composite was associated with an improvement of the thermal stability, morphological roughness, a 9–12% more hydrophobic behavior (composite C1, C5, and C8), increase in antibacterial activity from 23.2 to 33.1% for gram negative bacteria and from 37.28 to 40.59% for gram positive bacteria, and with a cell viability greater than 100% for 24 and 72 h. The films obtained can serve as a wound healing dressing and regenerating biomaterial.
“…Considering the interaction of the lysozyme and the polymer matrix, since O-CMQ is a polysaccharide, it is degraded by enzymatic hydrolysis. 13 In other studies, in scaffolds made with gelatin, chitosan, and hydroxyapatite, it was shown that the degradation increases with the incorporation of gelatin but decreases when the hydroxyapatite is added to the biomaterial, 22 a similar trend was observe in the composite films with Ag-Cu/HA, compared to the OCMC and gelatin control film. The changes in pH seen during the biodegradation period of the material were monitored during the 72h exposure of 1 X PBS with lysozyme (Figure 5(b)).…”
Section: Enzymatic Degradationmentioning
confidence: 73%
“…Considering the interaction of the lysozyme and the polymer matrix, since O-CMQ is a polysaccharide, it is degraded by enzymatic hydrolysis. 13 Figure 5.Degradation enzymatic results: (a) the weight loss percentage of de films during enzymatic degradation on 1, 2, and 3 days, data represent mean ± S.D. ( n = 5), (b) pH variation of the PBA solution during enzymatic degradation on 1, 2 and 3 days, (c) Observation of the morphology and chemical composition of the surface by SEM-EDS of the C9 sample: (c1) day 1, (c2) day 2 and, (c3) day 3.Figure 6.(a) Antibacterial properties films result on Gram – and Gram + bacteria at 24 h, (b) Cell viability percentage of fibroblasts seeded on the films at 24 and 72 h. Data represents mean ± S.D.…”
Wound dressing composite films of O-carboxymethyl chitosan (OCMC) and gelatin were prepared and mixed with hydroxyapatite (HA) composited with Silver (Ag) and Copper (Cu) at different concentrations. The chemical, thermal, morphological, and biological properties of the composite films were studied. The analysis by FTIR confirmed the presence of interactions between gelatin and OCMC, and at the same time, the polymer matrix interactions with Ag-Cu/HA complex. The inclusion of nanoparticle to the composite was associated with an improvement of the thermal stability, morphological roughness, a 9–12% more hydrophobic behavior (composite C1, C5, and C8), increase in antibacterial activity from 23.2 to 33.1% for gram negative bacteria and from 37.28 to 40.59% for gram positive bacteria, and with a cell viability greater than 100% for 24 and 72 h. The films obtained can serve as a wound healing dressing and regenerating biomaterial.
“…To cross-link the samples, a solution of genipin (98% CBC) 10 millimolar was prepared using concentrated ethanol, which was added to the composites for 10 min and five times rinsed with ethanol. 25 The pH of the rinse water was measured to ensure NaOH removal and final neutralization. Finally, samples were lyophilized for 12 h.…”
Allium cepa extracts (AC) allow the fabrication of a biomaterial that, together with chitosan and PLGA, could be osteoconductive and promote a better and faster regeneration of bone tissue, with biocompatibility and biomineralization properties. In this work, scaffolds were developed by the thermally induced phase separation (TIPS) technique. An in vitro bioactivity analysis was performed using simulated body fluid (SBF). Scanning electron microscopy (SEM), energy dispersion spectroscopy, and infrared spectroscopy were used for the scaffolds characterization. The results showed a structure with a pore size distribution between 50 and 100 μm, which allowed the uniform formation of biological apatite crystals on the surface of the scaffolds. The chitosan/policaprolactone/ Allium cepa scaffold (ChPAC) showed the most promising results with a ratio of P/Ca between 1.6 and 1.7, a value very close to that of hydroxyapatite.
“…Chitosan has ideal mechanical properties and antibacterial activity. 9 Earlier study reported that collagen and chitosan composites can improve dressing performance. 10,11 However, the poor ability of this polymer to enhance wound healing has greatly limited its further application in clinical treatment.…”
Section: Introductionmentioning
confidence: 99%
“…Cumulative heparin release from CT-CL/Hp sponge in vitro. The amount of heparin was quantified at different time points(1,2,3,4,5,7,9,12,16, and 20 days). Three parallel samples were set for each group.…”
The wound dressing can temporarily replace the skin and play a protective role in the process of wound healing, preventing wound infection and inflammation, and providing a favorable environment for wound healing. In this study, a mixture of collagen and chitosan was lyophilized to be the host material of the sponge. This sponge was soaked into 1-ethyl-(dimethylaminopropyl) carbodiimide/N-hydroxy sulfosuccinimide cross-linking solution containing heparin and experienced secondary lyophilization to prepare the heparinized sponge (CT-CL/Hp). The surface morphology and structural characterization of the sponge was characterized by scanning electron microscope and Fourier transform infrared spectrometer, respectively. Relatively favorable water absorption capability were observed by measuring the physical properties. Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro. The sustained-release property of heparin from the sponges was measured using Alcian Blue assay. Experiments in vitro and in vivo showed that the sponges had satisfactory biocompatibility and lower sensitization. Moreover, the effect of sponge on early stages of wound healing was evaluated by guinea pigs wound healing models. Analysis of wound healing rates and histological examination showed satisfactory results. CT-CL/Hp enhanced expression of growth factors, particularly VEGF and EGF at day 7. These results demonstrated that CT-CL/Hp–treated sponges benefit to wound skin healing and regeneration.
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