Chitosan/nanohydroxyapatite composite membranes via dynamic filtration for guided bone regeneration

Abstract

Chitosan/hydroxyapatite (HA) composite membranes were prepared by the coprecipitation method and a subsequent dynamic filtration and freeze-drying process. The influences of the HA content of the membranes on their phase and morphology, mechanical properties, and bioactivity were investigated. FTIR analysis revealed that chitosan and HA had good miscibility over a wide range of compositions. Needle-like HA nanocrystals with low crystallinity were uniformly embedded in the chitosan matrix. As the HA content was increased, the tensile strength of the membranes exhibited a steady decrease, while the elastic modulus increased by a factor of 2 when 20% HA was added. The results of the in vitro cell culture showed that the highest alkaline phosphatase level was achieved when 30% HA was contained in the composites.

SYNTHESIS OF HAp-CHITOSAN-PVA COMPOSITE AS INJECTABLE BONE SUBSTITUTE MATERIAL

“…The hydroxyl group (OH) of HAp appeared at 3431 cm -1 . The FTIR results showed that the phosphate groups are the dominant compounds presence of a very small amount of calcium carbonate 23 .…”

Chitosan and its composites: Properties for use in bone substitution

“…The method used affects the quality, especially the mechanical strength of the resulting scaffold, which depends on the bond of -NH 2 and -OH groups of chitosan with Ca 2+ hydroxyapatite and the ratio of CTS to Hap. 20,23 CTS/HAp composites are characterized by significantly better mechanical properties than both products individually. The highest compression strength of the CTS/HAp scaffold, 119.86 MPa, was obtained by the use of 30:70 CTS:HAp.…”

“…18 CTS/HAp composites are completely non-toxic and exhibit a significant increase in osteoblast activity and their deposition/penetration of the composite. [19][20][21][22] There are many methods for the synthesis of CTS/HAp, ranging from the simple mixing of natural HAp with CTS by means of freezing, lyophilization and hybridization as well as the use of state-of-the-art methods such as electrospinning. The method used affects the quality, especially the mechanical strength of the resulting scaffold, which depends on the bond of -NH 2 and -OH groups of chitosan with Ca 2+ hydroxyapatite and the ratio of CTS to Hap.…”

Temporomandibular Disorders and BIO-IPNs: in vitro approach to find molecular solution to biological problem

“…However, it has its own disadvantages: autografting often leads to complications in wound healing, additional surgery, donor pain and an inadequate supply of bone to fill the gap [133]. [134]. Very few compounds are classified as bioactive, biodegradable and osteoconductive.…”