Chitosan nanoparticles enhance the antibacterial activity of chlorhexidine in collagen membranes used for periapical guided tissue regeneration

Barreras, Uriel Soto; Méndez, Fernando Torres; Martínez, Rita Elizabeth Martínez; Valencia, Carolina Samano; Rodríguez, Pánfilo Raymundo Martínez; Rodríguez, Juan Pablo Loyola

doi:10.1016/j.msec.2015.09.085

Cited by 67 publications

(39 citation statements)

References 33 publications

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“…Down regulation of cytolysin and biofilm-forming genes after treatment with nanoparticles proves that the nanoparticles are able to modulate the expression of biofilm-associated genes, thereby rendering the bacteria susceptible to treatment. This demonstrates that chitosan-propolis nanoformulation is an ideal anti-biofilm agent that not only penetrates biofilms but also renders the bacteria susceptible to subsequent treatment with antibacterial agents [37, 38]. …”

Section: Resultsmentioning

confidence: 99%

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

et al. 2017

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Propolis obtained from bee hives is a natural substance with antimicrobial properties. It is limited by its insolubility in aqueous solutions; hence ethanol and ethyl acetate extracts of Malaysian propolis were prepared. Both the extracts displayed antimicrobial and anti-biofilm properties against Enterococcus faecalis, a common bacterium associated with hospital-acquired infections. High performance liquid chromatography (HPLC) analysis of propolis revealed the presence of flavonoids like kaempferol and pinocembrin. This study investigated the role of propolis developed into nanoparticles with chitosan for its antimicrobial and anti-biofilm properties against E. faecalis. Bacteria that grow in a slimy layer of biofilm are resistant to penetration by antibacterial agents. The use of nanoparticles in medicine has received attention recently due to better bioavailability, enhanced penetrative capacity and improved efficacy. A chitosan-propolis nanoformulation was chosen based on ideal physicochemical properties such as particle size, zeta potential, polydispersity index, encapsulation efficiency and the rate of release of the active ingredients. This formulation inhibited E. faecalis biofilm formation and reduced the number of bacteria in the biofilm by ~90% at 200 μg/ml concentration. When tested on pre-formed biofilms, the formulation reduced bacterial number in the biofilm by ~40% and ~75% at 200 and 300 μg/ml, respectively. The formulation not only reduced bacterial numbers, but also physically disrupted the biofilm structure as observed by scanning electron microscopy. Treatment of biofilms with chitosan-propolis nanoparticles altered the expression of biofilm-associated genes in E. faecalis. The results of this study revealed that chitosan-propolis nanoformulation can be deemed as a potential anti-biofilm agent in resisting infections involving biofilm formation like chronic wounds and surgical site infections.

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Section: Resultsmentioning

confidence: 99%

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

et al. 2017

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“…For active targeting of polymeric nanoparticles, the surface of polymeric nanoparticles is usually functionalized with specific antibodies and aptamer bacteriophage proteins providing specific identification for the detection of pathogens and interaction between the particles and pathogens. The reported studies reveal that both the active and passive targeting strategies to deliver antimicrobial agents with polymeric nanoparticles improve their activities compared to their free forms [35][36][37].…”

Section: Nanomaterials As Antibacterial Delivery Systemsmentioning

confidence: 99%

Current Approaches for Exploration of Nanoparticles as Antibacterial Agents

Karaman¹,

Manner²,

Fallarero³

et al. 2017

Antibacterial Agents

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The ascending anxiety regarding antimicrobial resistance as well as the recalcitrant nature of biofilm-associated infections call for the development of alternative strategies to treat bacterial diseases. Nanoparticles have been considered as one of the emerging and promising platforms in this respect. Their unique physical and chemical properties may lead to fine-tuned interactions between them and bacteria. In this chapter, we aim to provide an overview on the use of nanoparticles as antimicrobial agents. Both antibacterial and anti-biofilm activities of nanoparticles and their current approaches will be reviewed. The in vitro methods that are used to evaluate the potency of nanoparticles as antimicrobial agents will be discussed in detail.

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“…Targeting molecules can also be used for sensitive and specific identification strategies for detection of pathogens such as Staphylococcus aureus, Mycobacterium tuberculosis and Escherichia coli via aptamer recognition and fluorescently tagged silica nanoparticles [42][43][44]. Increasing evidence suggests that encapsulation of antimicrobial agents on PNPs enhances their activity [7,45,46]. In addition, recent data show that polymeric nanoparticles have the ability to penetrate biofilms [47] and that polymeric nanoparticles are able to improve the delivery of antibiotics to the bacterial cells embedded in biofilm matrix, thereby increasing the efficacy of the treatment [48].…”

Section: Mechanism Of Actionmentioning

confidence: 99%

Polymeric nanoparticles – a novel solution for delivery of antimicrobial agents

Michalak¹,

Głuszek²,

Piktel³

et al. 2016

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The increased prevalence of antibiotic-resistant pathogens requires additional efforts to develop new antimicrobial agents and alternative methods to prevent and treat infections. In response to this challenge, a variety of nanotechnology-based tools are currently being designed and thoroughly investigated. To date, a considerable number of studies have reported increased activity of antibiotic-conjugated polymeric nanoparticles against bacteria and fungi associated with various infections, including those caused by drug-resistant pathogens. Importantly, high biocompatibility of these structures coupled with enhanced biological activity and improved pharmacokinetic properties supports the potential of these nanosystems as new tools to treat infections. In this review, we summarize the synthesis of polymer-based nanoparticles and describe their mechanism of action. We also highlight the recent advances in the application of antibiotic-conjugated polymeric nanoparticles as novel antimicrobial agents. StreszczenieStale narastająca lekooporność drobnoustrojów wymusza konieczność rozwoju alternatywnych metod profilaktyki i terapii zakażeń, a także uzasadnia prace nad nowymi lekami o aktywności przeciwdrobnoustrojowej. Realizacja tego celu jest moż-liwa dzięki zastosowaniu osiągnięć z zakresu nanotechnologii. Zwiększająca się liczba doniesień literaturowych potwierdza znaczną aktywność przeciwdrobnoustrojową nanocząstek polimerowych skoniugowanych z klasycznymi antybiotykami, co może zostać wykorzystane w terapii infekcji o charakterze bakteryjnym i grzybiczym, również tych wywoływanych przez patogeny lekooporne. Wysoka biokompatybilność nanocząstek polimerowych, a także możliwość zwiększania aktywności przeciwdrobnoustrojowej i poprawy parametrów farmakokinetycznych stosowanych obecnie chemioterapeutyków, wskazuje na możliwość zastosowania nanostruktur w nowoczesnej terapii chorób infekcyjnych. W niniejszej pracy podsumowano metody syntezy nanosystemów opartych na nanocząstkach polimerowych, a także opisano mechanizm ich działania. Przedstawione zostały również najnowsze osiągnięcia w syntezie nanocząstek skoniugowanych z antybiotykami, pozwalające na ich zastosowanie w terapii infekcji o charakterze bakteryjnym i grzybiczym.

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Chitosan nanoparticles enhance the antibacterial activity of chlorhexidine in collagen membranes used for periapical guided tissue regeneration

Cited by 67 publications

References 33 publications

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

Chitosan-propolis nanoparticle formulation demonstrates anti-bacterial activity against Enterococcus faecalis biofilms

Current Approaches for Exploration of Nanoparticles as Antibacterial Agents

Polymeric nanoparticles – a novel solution for delivery of antimicrobial agents

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